Hybrid bioinks for embedded bioprinting of an artery model DOI Creative Commons
Uxue Aizarna-Lopetegui, Ada Herrero-Ruiz, Monize Caiado Decarli

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract The integration of biomaterials with living cells and stimuli-responsive materials can be employed to create bioinks capable generating 3D in vitro models that better recapitulate native tissues. We introduce a multilayered artery model combines hybrid multifunctional including polymeric ink mimic the tunica adventitia arterial wall, an extracellular matrix (ECM)-based bioink for media layer. layer integrates inorganic (plasmonic nanoparticles) organic (polymers) components, providing structural support introducing diverse functionalities system. cell-laden consists human vascular smooth muscle (vSMC) within hydrogel based on porcine artery-derived decellularized (dECM) fosters optimal cell growth proliferation. An embedding bioprinting technique was fabrication multimaterial consisting concentric cylinders. dimensions parameters were fine-tuned ensure effective crosslinking multiple layers resulting creation self-supporting constructs. demonstrate effectiveness composition supporting viability proliferation construct, expanding possibilities employing novel multi-component vasculature resembling structure blood vessels.

Language: Английский

Microfluidic Human Physiomimetic Liver Model as a Screening Platform for Drug Induced Liver Injury DOI
Souradeep Dey, Amritha Bhat, G. Janani

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 310, P. 122627 - 122627

Published: May 21, 2024

Language: Английский

Citations

11

Lithography-based 3D printing of hydrogels DOI
Abhishek P. Dhand, Matthew D. Davidson, Jason A. Burdick

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Language: Английский

Citations

11

Fibroblast alignment and matrix remodeling induced by a stiffness gradient in a skin-derived extracellular matrix hydrogel DOI Creative Commons
Fenghua Zhao, Meng Zhang, Mehmet Nizamoglu

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 182, P. 67 - 80

Published: May 13, 2024

Large skin injuries heal as scars. Stiffness gradually increases from normal to scar tissue (20x higher), due excessive deposition and crosslinking of extracellular matrix (ECM) mostly produced by (myo)fibroblasts. Using a custom mold, skin-derived ECM hydrogels (dECM) were UV crosslinked after diffusion ruthenium (Ru) produce Ru-dECM gradient hydrogel. The Ru equates stiffness models physiology the scarred skin. Crosslinking in results 23-fold increase similar that Collagen fiber density stiffness-dependent fashion while stress relaxation also alters, with one additional Maxwell element necessary for characterizing Ru-dECM. Alignment fibroblasts encapsulated suggests directs orientate at ∼45 ° regions below 120 kPa. In areas above kPa, decrease prior adjusting their orientation. Furthermore, remodel surrounding gradient-dependent fashion, rearrangement cell-surrounding high-stiffness areas, formation interlaced collagen bundles low-stiffness areas. Overall, this study shows local environment generate an optimal mechanical topographical environment. This developed versatile vitro model using hydrogel unchanged biochemical Ruthenium crosslinking, 20-fold was achieved observed fibrotic interaction between depends on changes stiffness. directed alignment ∼45° with≤ cells higher decreased first then oriented themselves. remodeled regulated its mechanics reach condition. Our highlights dynamic interplay matrix, shedding light potential mechanisms strategies target remodeling.

Language: Английский

Citations

9

Developing 3D bioprinting for organs-on-chips DOI
Zhuhao Wu, Rui Liu, Ning Shao

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Organs-on-chips (OoCs) can be directly fabricated by 3D bioprinting techniques, which enhance the structural and functional fidelity of organ models broaden applications OoCs.

Language: Английский

Citations

1

Hybrid bioinks for embedded bioprinting of an artery model DOI Creative Commons
Uxue Aizarna-Lopetegui, Ada Herrero-Ruiz, Monize Caiado Decarli

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract The integration of biomaterials with living cells and stimuli-responsive materials can be employed to create bioinks capable generating 3D in vitro models that better recapitulate native tissues. We introduce a multilayered artery model combines hybrid multifunctional including polymeric ink mimic the tunica adventitia arterial wall, an extracellular matrix (ECM)-based bioink for media layer. layer integrates inorganic (plasmonic nanoparticles) organic (polymers) components, providing structural support introducing diverse functionalities system. cell-laden consists human vascular smooth muscle (vSMC) within hydrogel based on porcine artery-derived decellularized (dECM) fosters optimal cell growth proliferation. An embedding bioprinting technique was fabrication multimaterial consisting concentric cylinders. dimensions parameters were fine-tuned ensure effective crosslinking multiple layers resulting creation self-supporting constructs. demonstrate effectiveness composition supporting viability proliferation construct, expanding possibilities employing novel multi-component vasculature resembling structure blood vessels.

Language: Английский

Citations

1