bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
Abstract
The
integration
of
biomaterials
with
living
cells
and
stimuli-responsive
materials
can
be
employed
to
create
bioinks
capable
generating
3D
in
vitro
models
that
better
recapitulate
native
tissues.
We
introduce
a
multilayered
artery
model
combines
hybrid
multifunctional
including
polymeric
ink
mimic
the
tunica
adventitia
arterial
wall,
an
extracellular
matrix
(ECM)-based
bioink
for
media
layer.
layer
integrates
inorganic
(plasmonic
nanoparticles)
organic
(polymers)
components,
providing
structural
support
introducing
diverse
functionalities
system.
cell-laden
consists
human
vascular
smooth
muscle
(vSMC)
within
hydrogel
based
on
porcine
artery-derived
decellularized
(dECM)
fosters
optimal
cell
growth
proliferation.
An
embedding
bioprinting
technique
was
fabrication
multimaterial
consisting
concentric
cylinders.
dimensions
parameters
were
fine-tuned
ensure
effective
crosslinking
multiple
layers
resulting
creation
self-supporting
constructs.
demonstrate
effectiveness
composition
supporting
viability
proliferation
construct,
expanding
possibilities
employing
novel
multi-component
vasculature
resembling
structure
blood
vessels.
Acta Biomaterialia,
Journal Year:
2024,
Volume and Issue:
182, P. 67 - 80
Published: May 13, 2024
Large
skin
injuries
heal
as
scars.
Stiffness
gradually
increases
from
normal
to
scar
tissue
(20x
higher),
due
excessive
deposition
and
crosslinking
of
extracellular
matrix
(ECM)
mostly
produced
by
(myo)fibroblasts.
Using
a
custom
mold,
skin-derived
ECM
hydrogels
(dECM)
were
UV
crosslinked
after
diffusion
ruthenium
(Ru)
produce
Ru-dECM
gradient
hydrogel.
The
Ru
equates
stiffness
models
physiology
the
scarred
skin.
Crosslinking
in
results
23-fold
increase
similar
that
Collagen
fiber
density
stiffness-dependent
fashion
while
stress
relaxation
also
alters,
with
one
additional
Maxwell
element
necessary
for
characterizing
Ru-dECM.
Alignment
fibroblasts
encapsulated
suggests
directs
orientate
at
∼45
°
regions
below
120
kPa.
In
areas
above
kPa,
decrease
prior
adjusting
their
orientation.
Furthermore,
remodel
surrounding
gradient-dependent
fashion,
rearrangement
cell-surrounding
high-stiffness
areas,
formation
interlaced
collagen
bundles
low-stiffness
areas.
Overall,
this
study
shows
local
environment
generate
an
optimal
mechanical
topographical
environment.
This
developed
versatile
vitro
model
using
hydrogel
unchanged
biochemical
Ruthenium
crosslinking,
20-fold
was
achieved
observed
fibrotic
interaction
between
depends
on
changes
stiffness.
directed
alignment
∼45°
with≤
cells
higher
decreased
first
then
oriented
themselves.
remodeled
regulated
its
mechanics
reach
condition.
Our
highlights
dynamic
interplay
matrix,
shedding
light
potential
mechanisms
strategies
target
remodeling.
Lab on a Chip,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Organs-on-chips
(OoCs)
can
be
directly
fabricated
by
3D
bioprinting
techniques,
which
enhance
the
structural
and
functional
fidelity
of
organ
models
broaden
applications
OoCs.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
Abstract
The
integration
of
biomaterials
with
living
cells
and
stimuli-responsive
materials
can
be
employed
to
create
bioinks
capable
generating
3D
in
vitro
models
that
better
recapitulate
native
tissues.
We
introduce
a
multilayered
artery
model
combines
hybrid
multifunctional
including
polymeric
ink
mimic
the
tunica
adventitia
arterial
wall,
an
extracellular
matrix
(ECM)-based
bioink
for
media
layer.
layer
integrates
inorganic
(plasmonic
nanoparticles)
organic
(polymers)
components,
providing
structural
support
introducing
diverse
functionalities
system.
cell-laden
consists
human
vascular
smooth
muscle
(vSMC)
within
hydrogel
based
on
porcine
artery-derived
decellularized
(dECM)
fosters
optimal
cell
growth
proliferation.
An
embedding
bioprinting
technique
was
fabrication
multimaterial
consisting
concentric
cylinders.
dimensions
parameters
were
fine-tuned
ensure
effective
crosslinking
multiple
layers
resulting
creation
self-supporting
constructs.
demonstrate
effectiveness
composition
supporting
viability
proliferation
construct,
expanding
possibilities
employing
novel
multi-component
vasculature
resembling
structure
blood
vessels.
