Carbon
dot
(CD)
nanozymes
with
excellent
biocompatibility,
optical
properties,
and
catalytic
activity
show
great
promise
for
microbial
detection
drug
sensitivity
testing.
This
study
reports
the
synthesis
of
metal-doped
green-emitting
CDs
good
peroxidase-like
activity,
which
were
synthesized
via
a
one-step
hydrothermal
route
using
thiourea,
N-[3-(trimethoxysilyl)propyl]ethylenediamine,
catechol
as
starting
materials
FeCl3
doping
agent.
In
presence
H2O2,
catalyze
oxidation
3,3',5,5'-tetramethylbenzidine
(TMB),
producing
blue
product;
however,
in
bacteria
bacterial
catalase
enzyme
decomposes
H2O2
inhibits
CDs,
preventing
color
change.
The
neutralizes
prevents
from
color-changing
reaction
TMB.
Based
on
CDs-TMB-H2O2
cascade
system
bioenzymes
nanozymes,
we
developed
rapid,
sensitive,
direct
colorimetric
method
Staphylococcus
aureus
(S.
aureus)
limit
2
×
103
CFU/mL
linear
range
103-2
106
CFU/mL.
visual
was
successfully
applied
to
S.
food
samples.
Antibiotics
have
different
effects
proliferation
sensitive
resistant
strains,
leading
levels
hydrolysis
solution
resulting
varying
intensities
color;
therefore,
simple
antibiotic
susceptibility
test.
applications
CD
provide
powerful
tool
detecting
pathogenic
food,
clinical,
environmental
samples
infections.
ACS Sensors,
Год журнала:
2024,
Номер
9(5), С. 2429 - 2439
Опубликована: Апрель 26, 2024
Norovirus
(NoV)
stands
as
a
significant
causative
agent
of
nonbacterial
acute
gastroenteritis
on
global
scale,
presenting
substantial
threat
to
public
health.
Hence,
the
development
simple
and
rapid
analytical
techniques
for
NoV
detection
holds
great
importance
in
preventing
controlling
outbreak
epidemic.
In
this
work,
self-powered
photoelectrochemical
(PEC)
immunosensor
capsid
protein
(VP1)
was
proposed
by
π-electron-rich
carbon
nitride
homojunction
(ER-CNH)
photoanode.
C4N2
ring
derived
from
π-rich
locust
bean
gum
introduced
into
tri-s-triazine
structure,
creating
large
π-delocalized
conjugated
homojunction.
This
strategy
enhances
C/N
atomic
ratio,
which
widens
light
utilization,
narrows
bandgap,
optimizes
electronic
band
structure
nitride.
By
introduction
p-type
domains
were
induced
within
n-type
build
internal
electric
field
at
interface,
thus
forming
p–n
boost
carrier
separation
transfer.
The
ER-CNH
photoanode
exhibited
excellent
photoelectric
performance
water
oxidation
capacity.
Since
VP1
inhibits
photoanode,
open-circuit
potential
as-prepared
PEC
system
reduced
detecting
VP1.
achieved
remarkably
low
limit
(∼5
fg
mL–1)
displayed
high
stability
applicability
actual
stool
samples.
research
serves
foundation
concept
constructing
immunosensors
detect
other
viruses
promotes
application
systems
life
safety.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 23, 2024
Abstract
Photocatalytic
hydrogen
evolution
holds
great
promise
for
addressing
critical
energy
and
environmental
challenges,
making
it
an
important
area
in
scientific
research.
One
of
the
most
popular
photocatalysts
is
graphitic
carbon
nitride
(gCN),
which
has
emerged
as
a
noteworthy
candidate
generation
through
water
splitting.
However,
ongoing
research
aims
to
enhance
its
properties
practical
applications.
Herein,
we
introduce
green
approach
fabrication
porous
few-layered
gCN
with
surface
modifications
(such
oxygen
doping,
deposition,
nitrogen
defects)
promoted
performance
reaction.
The
process
involves
one-step
solvothermal
treatment
bulk
(bulk-gCN)
presence
different
sugars
(glucose,
sucrose,
fructose).
Interestingly,
conducted
time-dependent
revealed
that
exfoliated
fructose
at
180
°C
6
h
(fructose_6h)
exhibits
remarkable
13-fold
promotion
photocatalytic
compared
bulk-gCN.
studied
materials
were
extensively
characterized
by
microscopic
spectroscopic
techniques,
allowing
us
propose
reaction
mechanism
during
water-splitting
over
fructose_6h.
Furthermore,
study
highlights
potential
employing
facile
environmentally
friendly
fructose-assisted
improve
efficiency
stability
catalysts
based
on
nitride.
