ACS Applied Nano Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 21, 2024
Non-small
cell
lung
cancer
(NSCLC)
is
the
most
prevalent
type
of
cancer,
characterized
by
a
relatively
slow
growth
rate
and
propensity
toward
distant
metastases
following
local
spread.
These
factors
present
significant
challenges
in
both
diagnosis
treatment
NSCLC,
particularly
for
early
stage
NSCLC.
In
this
study,
based
on
self-assembled
functionalized
tetrahedral
DNA
nanostructure
(TDN),
multifunctional
drug
delivery
system
has
been
constructed
NSCLC
adenocarcinoma
line
A549
(A549
cells)
to
achieve
intracellular
imaging
combined
delivery.
The
TDN
was
vitro
four
vertices
were
with
Cy5,
s6
aptamer,
p28
cell-penetrating
peptide
siRNA,
respectively.
Subsequently,
daunorubicin
(DAU)
loaded
obtain
DAU@TDN-p28-s6-siRNA
through
intercalation
double-stranded
DNA.
While
Cy5
employed
tracking
imaging,
aptamer
used
target
cells
enrich
around
cells,
can
facilitate
entrance
into
via
endocytosis.
Once
taken
inside
cell,
modified
siRNA
bind
mRNA
guiding
translation
ERBB3
protein
down-regulate
its
expression,
ultimately
leading
inhibition
growth.
Simultaneously,
targeted
delivery,
DAU
accurately
inhibits
tumor
activity,
thus
achieving
synergy
inhibit
cells.
experiments
have
demonstrated
that
compared
control,
reduces
expression
71.27%,
viability
37.84%,
as
well
exhibits
high
targeting
specificity.
Therefore,
development
assemblies
provides
an
efficient
way
potential
biomedical
field.
Heliyon,
Год журнала:
2024,
Номер
10(16), С. e36258 - e36258
Опубликована: Авг. 1, 2024
Natural
bone
tissue
has
the
certain
function
of
self-regeneration
and
repair,
but
it
is
difficult
to
repair
large
damage.
Recently,
although
autologous
grafting
"gold
standard"
for
improving
high
cost,
few
donor
sources.
Besides,
allogeneic
causes
greater
immune
reactions,
which
hardly
meet
clinical
needs.
The
engineering
(BTE)
been
developed
promote
repair.
Gelatin,
due
its
biocompatibility,
receives
a
great
deal
attention
in
BTE
research
field.
However,
disadvantages
natural
gelatin
are
poor
mechanical
properties
single
structural
property.
With
development
BTE,
often
used
combination
with
range
natural,
synthetic
polymers,
inorganic
materials
achieve
synergistic
effects
complex
physiological
process
review
delves
into
fundamental
structure
unique
gelatin,
as
well
excellent
necessary
scaffold
materials.
Then
this
explores
application
modified
three-dimensional
(3D)
scaffolds
various
structures
including
3D
fiber
scaffolds,
hydrogels,
nanoparticles.
In
addition,
focuses
on
efficacy
composite
consisting
or
polymeric
materials,
bioactive
ceramics
metallic/non-metallic
defects.
these
gelatin-based
provides
new
ideas
design
good
biosafety.
JACS Au,
Год журнала:
2024,
Номер
4(6), С. 2381 - 2392
Опубликована: Май 30, 2024
Extracellular
vesicles
(EVs)
are
naturally
occurring
secreted
by
cells
that
can
transport
cargo
between
cells,
making
them
promising
bioactive
nanomaterials.
However,
due
to
the
complex
and
heterogeneous
biological
characteristics,
a
method
for
robust
EV
manipulation
efficient
delivery
is
still
lacking.
Here,
we
developed
novel
class
of
extracellular
vesicle
spherical
nucleic
acid
(EV-SNA)
nanostructures
with
scalability,
programmability,
cellular
delivery.
EV-SNA
was
constructed
through
simple
hydrophobic
coassembly
natural
EVs
cholesterol-modified
oligonucleotides
be
stable
1
month
at
room
temperature.
Based
on
programmable
shells,
respond
AND
logic
gates
achieve
assembly
manipulation.
Importantly,
from
wide
range
sources
EV,
enhancing
capability
nearly
10–20
times.
Compared
artificial
liposomal
SNA,
endogenous
exhibited
better
biocompatibility
more
effective
antisense
in
hard-to-transfect
primary
stem
cells.
Additionally,
deliver
functional
immune
regulation.
As
material
form,
may
provide
modular
framework
paradigm
EV-based
applications
drug
delivery,
disease
treatment,
nanovaccines,
other
fields.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
Osteoporosis
(OP)
is
a
systemic
skeletal
disorder
characterized
by
decreased
bone
mineral
density
and
heightened
risk
of
fractures.
Therapies
for
OP
have
primarily
focused
on
balancing
formation
resorption,
but
enhancing
the
remineralization
osteoporotic
also
key
strategy
effective
repair.
Recent
insights
into
biomineralization
mechanisms
highlighted
essential
role
mineral-containing
extracellular
vesicles
(EVs)
secreted
osteoblasts
in
promoting
marrow
mesenchymal
stromal/stem
cell
(BMSC)
differentiation
initiating
matrix
mineralization.
Drawing
from
these
principles,
we
developed
biomimetic
approach
to
replicate
structure
function
osteoblast-derived
EVs
engineering
mitochondrial
minerals
with
homing
membranes
(CMs).
This
bone-targeted
system
exhibits
excellent
biocompatibility,
osteogenic
stimulating
angiogenesis
regulating
cellular
energy
metabolism.
Additionally,
CM-coated
shows
affinity
collagen
fibrils,
effectively
intrafibrillar
mineralization,
thereby
facilitating
Overall,
offers
safe
efficient
therapeutic
alternative,
positioning
it
as
platform
tissue
regenerative
medicine.
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
ABSTRACT
This
study
aimed
to
clarify
the
role
and
mechanism
of
tetrahedral
framework
nucleic
acids
(tFNAs)
in
regulating
M2
macrophages
reduce
intestinal
injury.
An
injury
model
was
established
by
intraperitoneal
injection
lipopolysaccharides
(LPS)
mice
explore
alleviating
effects
tFNAs
on
Inflammatory
factors
were
detected
quantitative
polymerase
chain
reaction
(qPCR)
enzyme‐linked
immunosorbent
assay
(ELISA).
The
barrier
permeability
assessed
using
western
blotting
immunohistochemistry.
Macrophages
gut
localised
quantified
immunofluorescence.
Western
used
investigate
inflammation
injured
intestines.
These
results
show
that
attenuated
sepsis‐induced
can
also
promote
reconstruction
permeability.
In
vivo,
accelerated
aggregation
at
an
early
stage
reduced
number
M1
intestine.
addition,
enhanced
clearance
ability
macrophages.
They
activated
signalling
transcription
activating
factor
1(STAT1)
cytokine
inhibitory
1/3
(SOCS1/3)
pathways
increasing
expression
phagocytic
receptor
Mertk.
findings
indicated
alleviate
macrophages,
providing
a
new
option
for
treating
Abstract
Oral
mucosal
injuries
are
commonly
caused
by
factors
such
as
trauma,
infection,
or
inflammation,
especially
in
diabetic
patients
where
healing
is
difficult
and
significantly
affects
quality
of
life.
In
this
study,
a
nanocarrier
system
based
on
DNA
tetrahedrons
(TDN)
developed,
which
serve
ideal
vectors
due
to
their
excellent
intracellular
uptake
drug
delivery
capabilities.
By
efficiently
delivering
miR132
into
cells,
the
proliferation
migration
human
oral
fibroblasts
(HOMFs)
umbilical
vein
endothelial
cells
(HUVECs)
regulated,
along
with
modulation
inflammation
antioxidant
processes.
wound
model
rats,
miR@TDN
effectively
stably
delivers
injured
mucosa.
regulating
inflammatory
response,
promoting
blood
vessel
regeneration,
enhancing
defense
mechanisms,
significant
improvement
cellular
repair
function
acceleration
process
observed.
These
findings
provide
new
strategy
experimental
basis
for
clinical
treatment
injuries.
