Electrochemiluminescence Lateral Flow Immunoassay Using Ruthenium(II) Complex‐Loaded Dendritic Mesoporous Silica Nanospheres for Highly Sensitive and Quantitative Detection of SARS‐CoV‐2 Nucleocapsid Protein

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Авг. 5, 2024

Abstract Lateral flow immunoassays (LFIA) are widely used for the cost‐effective and rapid detection of diverse analytes. However, traditional LFIA suffers from difficulties in providing quantitative results has low sensitivity. Herein, is combined with electrochemiluminescence (ECL), a leading transduction technique high sensitivity wide dynamic range, to achieve highly sensitive severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS‐CoV‐2 N protein). Ruthenium(II)‐based complexes synthesized loaded into dendritic mesoporous silica nanospheres (PEI‐Ru/dSiO 2 ), which possessed central‐radial pore channels served as tags ECL‐LFIA. The electrodes fabricated on nitrocellulose (NC) membrane, simplifies structure PEI‐Ru/dSiO captured electrode surface via sandwich immunoreaction, enhances ECL signal by decreasing distance between surface. Using 2,2‐bis(hydroxymethyl)‐2,2′,2′'‐nitrilotriethanol (BIS‐TRIS) coreactant, ECL‐LFIA detecting SARS‐CoV‐2 protein, linear range 1–10 4 ng mL −1 limit (LOD) 0.52 . can also be detect analytes complex matrices. These demonstrate that prepared great potential point‐of‐care testing platform disease biomarkers.

Язык: Английский

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Ferrous Tungstate Nanomaterials with Excellent Enzyme-Mimicking Activity to Enhance Lateral Flow Immunoassay Sensitivity

Analytical Chemistry, Год журнала: 2025, Номер 97(5), С. 2714 - 2723

Опубликована: Янв. 30, 2025

Lateral flow immunochromatography (LFIA) with gold nanoparticles (AuNPs) is widely used in the biomedical field as a rapid and simple vitro detection technique. However, conventional AuNP-LFIA has limitations sensitivity range. In this study, nonprecious metal iron-based bimetallic FeWO4 nanomaterials convenient excellent enzyme-mimetic catalytic activities were synthesized by one-pot hydrothermal method. Here, combined C-reactive protein (CRP) antibodies to form novel signal-enhancing probe for analysis of CRP. The further achieves significant signal amplification catalyzing oxidation reaction 3-amino-9-ethylcarbazole LFIA assay, thereby improving accuracy assay. application FeWO4-based improved limit CRP 19.38 ng/mL after amplification, which approximately 30-fold lower than that addition, method demonstrated good stability reproducibility, providing promising prospective strategy early diagnosis inflammatory diseases.

Язык: Английский

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Smartphones as a platform for molecular analysis: concepts, methods, devices and future potential

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Smartphones have the potential to transform chemical and biological analysis by functioning as portable, versatile, democratized platforms for molecular diagnostics. Recent advances in optical detection devices are reviewed.

Язык: Английский

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Bidirectionally Favorable Platform: A Dual-Targeting Probe-Encoded Maple Leaf-Type Fluorescent Lateral Flow Immunoassay for Multiple Biomarker Detection

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

In the traditional multiplexed lateral flow immunoassay (LFIA), different detection probes against targets are necessary. However, relative complexity and high cost of probe preparation, as well insufficient user-friendliness, limit application LFIA in disease diagnosis. Here, we reported a bidirectionally favorable (BDF-LFIA) platform to maximize convenience for both manufacturers users. Red-emitting time-resolved fluorescent nanoparticles were coated with antibodies recognize multiple simultaneously, which greatly simplified preparation by manufacturers. Ultrabright green-emitting gold nanoclusters pre-embedded on test line reference signal achieve target concentration-dependent maple leaf-type hue readout from green yellow red, was quite user-friendly. Taking cancer biomarkers alpha-fetoprotein carcinoembryonic antigen examples, this assay achieved visual 2 ng/mL. Compared conventional LFIA, BDF-LFIA could generate more discernible around threshold concentration targets. Moreover, successfully diagnosed 54 clinical samples. Overall, showed bidirectional benefits users provided new concept detection.

Язык: Английский

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Dual-readout fluorescence ‘turn-on’ lateral flow immunoassay for sensitive detection of imidacloprid

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117379 - 117379

Опубликована: Март 1, 2025

Язык: Английский

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Enhancing lateral flow immunoassay performance for cardiac troponin I detection with pore-size tailored silica nanoparticles and smartphone-based “AdaptiScan” analysis

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Март 26, 2025

The accurate and rapid detection of cardiac troponin I (cTnI) at the point care is crucial for timely diagnosis myocardial infarction (MI). This study introduces an advanced lateral flow immunoassay (LFIA) platform cTnI detection. We employed small-sized, large-pore dendritic mesoporous silica nanoparticles (DMSN-2) to encapsulate quantum dots (QDs), achieving enhanced QD loading capacity 1.427 g QD/g silica, compared 0.881 smaller pore counterparts (DMSN-1). nano-LFIA was further integrated with “AdaptiScan”, a smartphone-based system that uses adaptive algorithms automatically extract analyze fluorescence signals from LFIA strips. integration pore-size tailored DMSNs “AdaptiScan” resulted in limit 42.6 ng/L, which meets clinical diagnostic requirements. offers sensitive, cost-effective, portable solution MI, potentially transforming point-of-care testing resource-limited settings.

Язык: Английский

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Lateral flow immunoassay using plasmonic scattering

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 9, 2025

The lateral flow immunoassay (LFIA) is one of the most successful sensing platforms for real-world point-of-care (POC) testing. However, achieving PCR-level sensitivity without compromising inherent advantages LFIA, such as rapid and robust operation, affordability, naked-eye detection, has remained a primary challenge. In this study, plasmonic scattering-utilising LFIA was proposed, created by transparentising nitrocellulose membrane placing light-absorbing backing card under membrane. This minimised background signal from its matrix, leading to substantially enhanced enabling detection scattering gold nanoparticles optics. Our showed an approximately 2600-4400 times higher limit compared with that commercial LFIAs in influenza A assays. addition, it exhibited 90% clinical validation, approaching sensitivity, while 23-30% sensitivity. plays ground-breaking role POC diagnostics significantly boosts follow-up research.

Язык: Английский

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Wheatgrass-inspired MoSe2@Pt heterojunctions with enhanced catalytic and photothermal functionalities for highly sensitive pathogen diagnosis based on machine learning

Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 137020 - 137020

Опубликована: Ноя. 1, 2024

Язык: Английский

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Selective surface passivation of gold nanoparticles: A strategy for enhanced sensitivity in lateral flow immunoassay

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158571 - 158571

Опубликована: Дек. 1, 2024

Язык: Английский

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Self‐Sustained‐Release Strategy Realizes Colloid Oriented Assembly to Fabricate Prussian Blue with Hierarchical Structure

Small, Год журнала: 2024, Номер unknown

Опубликована: Июнь 12, 2024

Abstract The controlled self‐assembly of nanomaterials has been a great challenge in nanosynthesis, especially for hierarchical architectures with high complexity. Particularly, the structural design Prussian blue (PB) series materials robustness and fast nucleation is even more difficult. Herein, self‐sustained‐release strategy based on slow release metal ions from coordination proposed to guide assembly PB crystals. key this by ligand, which can create ultra‐low concentrations so as provide possibility realize surface charge manipulation primary colloids. By adding electrolyte or changing polarity solution, regulation colloid realized, structures branch fractal structure (PB‐BS), octahedral structure, spherical are effectively constructed. This work not only achieves designability but also synchronizes functionalization during growth process situ encapsulation effective catalytic active component L‐Ascorbic acid. As result, assembled PB‐BS exhibits greatly enhanced activity selectivity styrene oxidation oxidized increasing 35.6% 80.5% (PB‐BS).

Язык: Английский

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