Oxygen-Generating Biomaterials for Translational Bone Regenerative Engineering

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(44), С. 50721 - 50741

Опубликована: Март 29, 2023

Successful regeneration of critical-size defects remains one the significant challenges in regenerative engineering. These large-scale bone are difficult to regenerate and often reconstructed with matrices that do not provide adequate oxygen levels stem cells involved process. Hypoxia-induced necrosis predominantly occurs center large since host tissue's local vasculature fails sufficient nutrients oxygen. Indeed, utilizing oxygen-generating materials can overcome central hypoxic region, induce tissue in-growth, increase quality life for patients extensive damage. This article reviews recent advances biomaterials translational We discussed different oxygen-releasing delivery methods, fabrication methods matrices, biology, oxygen's role regeneration, emerging new could potentially be used

Язык: Английский

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Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 2, 2025

Mesenchymal stem cells (MSCs) play a critical role in cell therapy due to their tissue-mimicking abilities. However, conventional 2D culture conditions often lead the loss of native hypoxic niche, potentially limiting therapeutic efficacy. 3D bioprinting offers method recreate intricate biological environments by integrating with extracellular matrices. Therefore, it is essential adapt printing techniques accurately replicate MSCs' ecological facilitating integration technology into clinical applications. In this study, we optimized capabilities using performed cellular aggregates (PCA) method. We observed that printed matrix creates microenvironment, resulting significant increase level production several paracrine signaling molecules and immunomodulatory factors MSCs. Furthermore, MSCs exhibited enhanced stemness proliferative capacity early stages culture. RNA-seq analysis revealed these changes behavior were associated environment created during procedure By optimizing bioink composition parameters, successfully simulated vivo leading notable improvements MSC characteristics capacity. RNA sequencing further confirmed activation hypoxia pathways, which are crucial for properties. These findings offer valuable insights leveraging MSC-based therapies regenerative medicine.

Язык: Английский

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The role of hypoxia in stem cell regulation of the central nervous system: From embryonic development to adult proliferation

CNS Neuroscience & Therapeutics, Год журнала: 2021, Номер 27(12), С. 1446 - 1457

Опубликована: Ноя. 24, 2021

Hypoxia is involved in the regulation of various cell functions body, including stem cells. The hypoxic microenvironment indispensable from embryonic development to regeneration and repair adult In addition cells, which need maintain their self-renewal properties pluripotency a environment, neural cells (NSCs), also exist microenvironment. subventricular zone (SVZ) hippocampal dentate gyrus (DG) are main sites neurogenesis brain. can promote proliferation, migration, maturation NSCs these regions. Also, because most neurons brain non-regenerative, transplantation considered as promising strategy for treating central nervous system (CNS) diseases. Hypoxic treatment increases effectiveness therapy. this review, we firstly describe role hypoxia different such NSCs, induced pluripotent discuss hypoxia-treated CNS diseases treatment. Furthermore, highlight mechanisms regulating SVZ DG proliferation other CNS.

Язык: Английский

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Self‐Oxygenation of Tissues Orchestrates Full‐Thickness Vascularization of Living Implants

Advanced Functional Materials, Год журнала: 2021, Номер 31(42)

Опубликована: Июль 6, 2021

Abstract Bioengineering of tissues and organs has the potential to generate functional replacement organs. However, achieving full‐thickness vascularization that is required for long‐term survival living implants remained a grand challenge, especially clinically sized implants. During pre‐vascular phase, implanted engineered are forced metabolically rely on diffusion nutrients from adjacent host‐tissue, which larger results in anoxia, cell death, ultimately implant failure. Here it reported this challenge can be addressed by engineering self‐oxygenating tissues, achieved via incorporation hydrophobic oxygen‐generating micromaterials into tissues. Self‐oxygenation transforms anoxic stresses hypoxic stimulation homogenous tissue size‐independent manner. The situ elevation oxygen tension enables sustained production high quantities angiogenic factors cells, offered protected pro‐angiogenic microenvironment. Numerical simulations predict self‐oxygenation will effectively orchestrate rapid empirically confirmed vivo experimentation. thus represents novel, effective, widely applicable strategy enable implants, expected advance organ transplantation regenerative medicine applications.

Язык: Английский

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Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13414 - 13414

Опубликована: Авг. 29, 2023

Osteoarthritis (OA) is the most common age-related degenerative joint disease. Inflammaging, linking inflammation and aging, found in senescent cells with secretions of matrix-degrading proteins proinflammatory cytokines. The senescence-associated secretory phenotype (SASP) plays a very important role OA progression. However, there remains no effective way to suppress progression, especially by suppressing inflammaging and/or chondrocyte SASP. Recent studies have shown that exosomes derived from hypoxia-cultured BMSCs can regenerate cartilage animal models. Some reports further indicated secreted MSCs contribute efficacy MSC therapy OA. whether ADSC-secreted (hypoxia-ADSC-Exos) alleviate SASP or progression unclear. Accordingly, we hypothesized hypoxia-ADSC-Exos beneficial effect on normal functions human articular chondrocytes (HACs), attenuate OA-like HACs vitro, rats. Hypoxia-ADSC-Exos were ADSCs cultured 1% O2 10% de-Exo-FBS for 48 h. molecular cell biological effects tested IL1-β-induced as treatment was ACLT-induced results showed had best GAG formation rather than those normoxia hypoxia plus 2% de-Exo-FBS. We alleviated harmful decreasing markers (GAG type II collagen) increasing fibrous (type I X collagen), matrix degradation enzymes (MMP13 ADAMT5), inflammatory cytokines (TNFα IL-6). More importantly, intra-articular suppressed evidenced weight-bearing function test quantification ACLT Moreover, through NGS bioinformatic analysis, seven potential miRNAs hypoxia-ADSC-Exos, which may regulating cellular oxidative stress attenuating senescence. In summary, demonstrated carrying potent miRNAs, not only improve HAC but also suggest hypoxia-ADSC-Exo offer another strategy future therapy.

Язык: Английский

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Application of hypoxia-mesenchymal stem cells in treatment of anaerobic bacterial wound infection: wound healing and infection recovery

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 5, 2023

Mesenchymal stromal cells, commonly referred to as MSCs, are a type of multipotent stem cells that typically extracted from adipose tissue and bone marrow. In the field engineering regenerative medicine, MSCs their exosomes have emerged revolutionary tools. Researchers now devoting greater attention because ability generate skin like fibroblasts keratinocytes, well distinctive potential decrease inflammation emit pro-angiogenic molecules at site wounds. More recent investigations revealed can exert numerous direct indirect antimicrobial effects immunologically mediated. Collectively, these properties remove bacterial infections when delivered in therapeutic setting. Regardless positive for multitude conditions, transplanted MSC cell retention continues be major challenge. Since administered into naturally hypoxic tissues, understanding impact hypoxia on functioning is crucial. Hypoxia has been postulated among factors determining differentiation resulting production inflammatory cytokines throughout process regeneration wound repair. This opened new horizons developing MSC-based systems potent tool oxygen-deprived regions, including anaerobic infection sites. review sheds light role hypoxia-MSCs treatment terms both activities.

Язык: Английский

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Insight into Hypoxia Stemness Control

Cells, Год журнала: 2021, Номер 10(8), С. 2161 - 2161

Опубликована: Авг. 22, 2021

Recently, the research on stemness and multilineage differentiation mechanisms has greatly increased its value due to potential therapeutic impact of stem cell-based approaches. Stem cells modulate their self-renewing capacities in response endogenous and/or extrinsic factors that can control cell fate. One key factor controlling phenotype is oxygen (O2). Several pieces evidence demonstrated complexity reproducing O2 physiological tensions gradients culture responsible for defective behavior vitro after transplantation. This still worsened by considering are conventionally incubated under non-physiological air tension (21%). Therefore, study signaling activated at lower tension, such as those existing native microenvironments (referred hypoxia), represent an effective strategy define if essential preserving naïve well modulating differentiation. Starting from this premise, goal present review report status art about link between hypoxia providing insight into factors/molecules involved, design targeted strategies that, recapitulating signals, enable towards use tissue engineering regenerative medicine.

Язык: Английский

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Extrusion bioprinting of cellular aggregates improves mesenchymal stem cell proliferation and differentiation

Biomaterials Advances, Год журнала: 2023, Номер 149, С. 213369 - 213369

Опубликована: Март 6, 2023

Язык: Английский

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Effect of in vivo culture conditions on the proliferation and differentiation of rat adipose-derived stromal cells

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 219, С. 111935 - 111935

Опубликована: Апрель 12, 2024

Язык: Английский

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Salvianolic acid B combined with bone marrow mesenchymal stem cells piggybacked on HAMA hydrogel re-transplantation improves intervertebral disc degeneration

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Сен. 27, 2022

Cell-based tissue engineering approaches have emerged as a realistic alternative for regenerative disc repair. The multidirectional differentiation potential of bone marrow mesenchymal stem cells (BMSCs) to treat degeneration intervertebral has also become viable option. We used 1% HAMA hydrogel carrier and co-encapsulated BMSCs Salvianolic acid B (SalB) into the reduce apoptosis transplanted cells. protective effect SalB on was first verified in vitro using CCK8 method, flow cytometry, Western-Blotting, physical properties biocompatibility hydrogels were vitro. rat model then established pinprick method taken at 4 8 W, examine extent by histology immunohistochemistry, respectively. It found that could effectively activating JAK2-STAT3 pathway. had larger pore size better water retention, percentage cell survival within significantly higher after addition hydrogels. In vivo setting, + group more pronounced delaying progression compared other treatment groups. this study encapsulate drugs with injection lesion research value field medicine.

Язык: Английский

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