Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(30)
Опубликована: Июнь 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Язык: Английский
Процитировано
41
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Фев. 3, 2025
The crosstalk between immunity and cancer in the regulation of tumor growth is considered a hallmark cancer. Antitumor refers to innate adaptive immune responses that regulate development proliferation. Tumor evasion represents major hindrance effective anticancer treatment. Extracellular vesicles (EVs) are nano-sized lipid-bilayer-enclosed particles secreted extracellular space by all cell types. They critically involved numerous biological functions including intercellular communication. Tumor-derived (TEVs) can transport variety cargo modulate cells microenvironment (TME). This review provides latest update about how evade surveillance exploiting TEVs. First, biogenesis EVs cargo-sorting machinery discussed. Second, differentiation, activation, function via TEVs illustrated. Last but not least, novel antitumor strategies reverse escape summarized.
Язык: Английский
Процитировано
3
Light Science & Applications, Год журнала: 2025, Номер 14(1)
Опубликована: Фев. 27, 2025
Optical sorting combines optical tweezers with diverse techniques, including spectrum, artificial intelligence (AI) and immunoassay, to endow unprecedented capabilities in particle sorting. In comparison other methods such as microfluidics, acoustics electrophoresis, offers appreciable advantages nanoscale precision, high resolution, non-invasiveness, is becoming increasingly indispensable fields of biophysics, chemistry, materials science. This review aims offer a comprehensive overview the history, development, perspectives various categorised passive active methods. To begin, we elucidate fundamental physics attributes both conventional exotic forces. We then explore sorting, which fuses diversity Raman spectroscopy machine learning. Afterwards, reveal essential roles played by deterministic light fields, configured lens systems or metasurfaces, particles based on their varying sizes shapes, resolutions speeds. conclude our vision most promising futuristic directions, AI-facilitated ultrafast bio-morphology-selective It can be envisioned that will inevitably become revolutionary tool scientific research practical biomedical applications.
Язык: Английский
Процитировано
3
Analytica Chimica Acta, Год журнала: 2024, Номер 1296, С. 342337 - 342337
Опубликована: Фев. 6, 2024
Язык: Английский
Процитировано
13
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Plasma-derived extracellular vesicles (EVs) are promising sources of biomarkers. It is still a challenge to isolate EVs from small amount human plasma for downstream proteomic analysis. The isolation process hindered by contamination with high-abundance blood proteins and lipoprotein particles, which adversely impact analyses. Moreover, although EV immune-isolation via magnetic beads often integrates flow sorting Western blotting (WB), it lacks compatibility nanoparticle tracking analysis (NTA) To address these issues, we have developed functional affinity bead, EVlent (Extracellular Vesicles isoLated Efficiently, Naturally, Totally), enabling the rapid efficient plasma. By optimizing quantities used, characterized isolated through WB, NTA, transmission electron microscopy (TEM), showing successful Proteomic identified over 2000 15,000 peptides 100 μL nearly 1000 trace samples as 5 μL. Additionally, this method significantly reduced contaminants, including lipoproteins, compared ultracentrifugation. Finally, applied strategy healthy individuals those Parkinson's disease, identifying four potential biomarkers that provide guidance clinical diagnosis.
Язык: Английский
Процитировано
1
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 12, 2025
The ultrasensitive detection of microRNAs (miRNAs) in extracellular vesicles (EVs) can accurately reflect the progress and metastasis miRNA-mediated intercellular communication, providing an unprecedented opportunity for liquid biopsy. However, due to low abundance high heterogeneity miRNAs EVs, quantification establishment a distribution model miRNA within native EVs remain challenging. Here, we have developed DNA tetrahedron-based single-molecule fluorescence imaging strategy overcome this challenge. internalization efficiency probe was as 70% without disrupting structure combined with imaging, achieved situ analysis single-copy individual amplification first time. A new has been revealed by statistical copy number across multiple cell lines, characterized occupancy heterogeneous distribution. More importantly, found that drug resistance cancer cells promote increase resistance-related corresponding secreted, insights into EV sorting mechanisms. We anticipate technology will rapidly advance communication based on EVs.
Язык: Английский
Процитировано
1
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 1, 2025
Extracellular particles (EPs), including extracellular vesicles (EVs) and non-vesicular (NVEPs), are multimolecular biomaterials released by cells that play a crucial role in intercellular communication. Recently, new subtypes of EPs associated with central nervous system (CNS), such as exophers supermeres have been identified. These provide perspectives for understanding the pathological progression CNS disorders confer potential diagnostic value liquid biopsies neurodegenerative diseases (NDs). Moreover, emerged promising drug delivery vehicles targeted platforms CNS-specific therapies. In this review, we delineate landscape EP their roles pathophysiology diseases. We also review recent advances EP-based diagnosis NDs highlight importance analytical single-particle resolution exploitation biomarkers. Furthermore, summarize application engineered EVs treatment outline underexplored NVEPs novel therapeutic agents.
Язык: Английский
Процитировано
1
Talanta, Год журнала: 2023, Номер 257, С. 124307 - 124307
Опубликована: Фев. 4, 2023
piR-823 is a newly discovered colorectal cancer marker with high diagnostic efficacy. However, the current quantification methods have complicated operations and cost, which restrict its clinical application. Herein, metal-organic framework (MOF) UiO-66 prototype structure supports gold nanoclusters (Au NCs), Au NCs/UiO-66-NH2, were prepared as model nanobiosensing platform for ratiometric detection of exosomal piR-823. The rolling circle amplification process provides sensitivity ensures good accuracy sensor. Such biosensor showed wide linear range 0.04-4 pM, low limit 10.2 fM towards In addition, can be used an effective supplement to carcinoembryonic antigen (CEA) in diagnosis cancer. This study not only potentially valuable ratio fluorescence involving enzyme catalytic reaction, but also offers design blueprint further expansion nanotechnology diverse biological analysis.
Язык: Английский
Процитировано
19
Cancer Letters, Год журнала: 2024, Номер 593, С. 216960 - 216960
Опубликована: Май 17, 2024
Extracellular vesicles (EVs) have been the subject of an exponentially growing number studies covering their biogenesis mechanisms, isolation and analysis techniques, physiological pathological roles, clinical applications, such as biomarker therapeutic uses. Nevertheless, heterogeneity EVs both challenges our understanding them presents new opportunities for potential application. Recently, EV field experienced a wide range advances. However, also remain huge. This review focuses on recent progress difficulties encountered in practical use settings. In addition, we explored concept to acquire more thorough involvement cancer, specifically focusing fundamental nature EVs.
Язык: Английский
Процитировано
9
Micromachines, Год журнала: 2024, Номер 15(5), С. 630 - 630
Опубликована: Май 8, 2024
Extracellular vesicles (EVs) serve as vital messengers, facilitating communication between cells, and exhibit tremendous potential in the diagnosis treatment of diseases. However, conventional EV isolation methods are labor-intensive, they harvest EVs with low purity compromised recovery. In addition, drawbacks, such limited sensitivity specificity traditional analysis methods, hinder application clinical use. Therefore, it is urgent to develop effective standardized for isolating detecting EVs. Microfluidics technology a powerful rapidly developing that has been introduced solution above bottlenecks. It holds advantages high integration, short time, consumption samples reagents. this review, we summarize techniques alongside microfluidic-based methodologies detection We emphasize distinct microfluidic enhancing capture efficiency precise targeting extracellular (EVs). also explore its analytical role targeted detection. Furthermore, review highlights transformative impact on analysis, achieve automated high-throughput samples.
Язык: Английский
Процитировано
7