Recent Advances in Microfluidic-Based Extracellular Vesicle Analysis DOI Open Access
Jiming Chen, Meiyu Zheng,

Qiaoling Xiao

et al.

Published: March 13, 2024

Extracellular vesicles (EVs) hold enormous potential in the diagnosis and treatment of diseases, benefit from their roles intercellular signaling, as well plentiful presence stability body fluids. However, conventional EV isolation methods are labor-intensive, harvest EVs with low purity compromised recovery. In addition, drawbacks, such limited sensitivity specificity traditional analysis hinder application clinical use. Therefore, it is urgent to develop effective standardized for isolating detecting EVs. Microfluidics technology a powerful rapidly developing that has been introduced solution above bottlenecks. It holds advantages high integration, short time, consumption samples reagents. this review, we summarize techniques alongside microfluidic-based methodologies detection We emphasize separation microfluidic enhancing capture efficiency precise targeting, while summarizing its analytical role targeted discussing significance tumor microenvironment, providing direction achieving automated high-throughput samples.

Language: Английский

Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

Citations

37
Effect of Extracellular Vesicles Derived From Tumor Cells on Immune Evasion DOI Creative Commons

Xixi Liu,

Kenneth K.W. To,

Qinsong Zeng

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

The crosstalk between immunity and cancer in the regulation of tumor growth is considered a hallmark cancer. Antitumor refers to innate adaptive immune responses that regulate development proliferation. Tumor evasion represents major hindrance effective anticancer treatment. Extracellular vesicles (EVs) are nano-sized lipid-bilayer-enclosed particles secreted extracellular space by all cell types. They critically involved numerous biological functions including intercellular communication. Tumor-derived (TEVs) can transport variety cargo modulate cells microenvironment (TME). This review provides latest update about how evade surveillance exploiting TEVs. First, biogenesis EVs cargo-sorting machinery discussed. Second, differentiation, activation, function via TEVs illustrated. Last but not least, novel antitumor strategies reverse escape summarized.

Language: Английский

Citations

3
Optical sorting: past, present and future DOI Creative Commons
Meng Yang, Yuzhi Shi, Qinghua Song

et al.

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 27, 2025

Optical sorting combines optical tweezers with diverse techniques, including spectrum, artificial intelligence (AI) and immunoassay, to endow unprecedented capabilities in particle sorting. In comparison other methods such as microfluidics, acoustics electrophoresis, offers appreciable advantages nanoscale precision, high resolution, non-invasiveness, is becoming increasingly indispensable fields of biophysics, chemistry, materials science. This review aims offer a comprehensive overview the history, development, perspectives various categorised passive active methods. To begin, we elucidate fundamental physics attributes both conventional exotic forces. We then explore sorting, which fuses diversity Raman spectroscopy machine learning. Afterwards, reveal essential roles played by deterministic light fields, configured lens systems or metasurfaces, particles based on their varying sizes shapes, resolutions speeds. conclude our vision most promising futuristic directions, AI-facilitated ultrafast bio-morphology-selective It can be envisioned that will inevitably become revolutionary tool scientific research practical biomedical applications.

Language: Английский

Citations

2
Rapid automated extracellular vesicle isolation and miRNA preparation on a cost-effective digital microfluidic platform DOI
Zhaoduo Tong, Dawei Yang, Chuanjie Shen

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1296, P. 342337 - 342337

Published: Feb. 6, 2024

Language: Английский

Citations

13
Ultrasensitive Quantification of microRNA Copy Number in Individual Extracellular Vesicles Using DNA Tetrahedron-Based Single-Molecule Imaging DOI
Weifeng Liu, Hongwei Yang, Xiaolong Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

The ultrasensitive detection of microRNAs (miRNAs) in extracellular vesicles (EVs) can accurately reflect the progress and metastasis miRNA-mediated intercellular communication, providing an unprecedented opportunity for liquid biopsy. However, due to low abundance high heterogeneity miRNAs EVs, quantification establishment a distribution model miRNA within native EVs remain challenging. Here, we have developed DNA tetrahedron-based single-molecule fluorescence imaging strategy overcome this challenge. internalization efficiency probe was as 70% without disrupting structure combined with imaging, achieved situ analysis single-copy individual amplification first time. A new has been revealed by statistical copy number across multiple cell lines, characterized occupancy heterogeneous distribution. More importantly, found that drug resistance cancer cells promote increase resistance-related corresponding secreted, insights into EV sorting mechanisms. We anticipate technology will rapidly advance communication based on EVs.

Language: Английский

Citations

1
Ratiometric fluorescent detection of exosomal piRNA-823 based on Au NCs/UiO-66-NH2 and target-triggered rolling circle amplification DOI Creative Commons
Yao Tong,

Bingxin Guan,

Zhiwei Sun

et al.

Talanta, Journal Year: 2023, Volume and Issue: 257, P. 124307 - 124307

Published: Feb. 4, 2023

piR-823 is a newly discovered colorectal cancer marker with high diagnostic efficacy. However, the current quantification methods have complicated operations and cost, which restrict its clinical application. Herein, metal-organic framework (MOF) UiO-66 prototype structure supports gold nanoclusters (Au NCs), Au NCs/UiO-66-NH2, were prepared as model nanobiosensing platform for ratiometric detection of exosomal piR-823. The rolling circle amplification process provides sensitivity ensures good accuracy sensor. Such biosensor showed wide linear range 0.04-4 pM, low limit 10.2 fM towards In addition, can be used an effective supplement to carcinoembryonic antigen (CEA) in diagnosis cancer. This study not only potentially valuable ratio fluorescence involving enzyme catalytic reaction, but also offers design blueprint further expansion nanotechnology diverse biological analysis.

Language: Английский

Citations

18
Plant-derived extracellular vesicles -a novel clinical anti-inflammatory drug carrier worthy of investigation DOI Open Access

Mingyue Shao,

Xiao Jin,

Sixi Chen

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 169, P. 115904 - 115904

Published: Nov. 18, 2023

Plant-derived extracellular vesicles (PDEVs) have shown remarkable potential as sustainable, green, and efficient drug delivery nanocarriers. As natural nanoparticles containing lipids, protein, nucleic acids secondary metabolites, they received widespread attention a replacement for mammalian exosomes in recent years. In this review, the advances isolation, identification, composition, therapeutic effect, clinical application prospect were comprehensively reviewed, respectively. addition, specific modification strategies been listed focusing on inherent drawbacks of raw PDEVs like low targeting efficiency poor homogeneity. With emphasis their biology mechanism terms immune regulation, regulating oxidative stress promoting regeneration anti-inflammatory field value demonstrated by citing some typical examples, review about would provide broad fundamental vision in-depth exploration development plant-derived in-vivo anti-inflammation even other biomedical applications.

Language: Английский

Citations

16
Low-cost inertial microfluidic device for microparticle separation: A laser-Ablated PMMA lab-on-a-chip approach without a cleanroom DOI Creative Commons
Cristian F. Rodríguez, Paula Guzmán-Sastoque, Mónica Gantiva-Díaz

et al.

HardwareX, Journal Year: 2023, Volume and Issue: 16, P. e00493 - e00493

Published: Nov. 12, 2023

Although microparticles are frequently used in chemistry and biology, their effectiveness largely depends on the homogeneity of particle size distribution. Microfluidic devices to separate purify particles based have been developed, but many require expensive cleanroom manufacturing processes. A cost-effective, passive microfluidic separator is presented, capable efficiently sorting purifying spanning range 15 µm 40 µm. Fabricated from Polymethyl Methacrylate (PMMA) substrates using laser ablation, this device circumvents need for facilities. Prior fabrication, rigorous optimization device's design was carried out through computational simulations conducted COMSOL Multiphysics. To gauge its performance, chitosan were employed as a test case. The results notably promising, achieving precision 96.14 %. This quantitative metric underscores size-based separation. low-cost accessible offers pragmatic solution laboratories researchers seeking precise control over sizes, without constraints environments. innovation not only mitigates limitations tied traditional cleanroom-based fabrication also widens horizons various applications within realms biology.

Language: Английский

Citations

15
Extracellular Vesicles for Dental Pulp and Periodontal Regeneration DOI Creative Commons
Hongbin Lai, Jiaqi Li, Xiaoxing Kou

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(1), P. 282 - 282

Published: Jan. 14, 2023

Extracellular vesicles (EVs) are lipid bound particles derived from their original cells, which play critical roles in intercellular communication through cargoes, including protein, lipids, and nucleic acids. According to biogenesis release pathway, EVs can be divided into three categories: apoptotic (ApoVs), microvesicles (MVs), small (sEVs). Recently, the role of oral disease has received close attention. In this review, main characteristics described, classification, biogenesis, biomarkers, components. Moreover, therapeutic mechanism tissue regeneration is discussed. We further summarize current status pulp/periodontal discuss potential mechanisms. The pulp periodontal might involve promotion immunomodulatory capabilities. Furthermore, we highlight challenges translational use EVs. This review would provide valuable insights strategies dental regeneration.

Language: Английский

Citations

14
Recent Advances in Microfluidic-Based Extracellular Vesicle Analysis DOI Creative Commons
Jiming Chen, Meiyu Zheng,

Qiaoling Xiao

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(5), P. 630 - 630

Published: May 8, 2024

Extracellular vesicles (EVs) serve as vital messengers, facilitating communication between cells, and exhibit tremendous potential in the diagnosis treatment of diseases. However, conventional EV isolation methods are labor-intensive, they harvest EVs with low purity compromised recovery. In addition, drawbacks, such limited sensitivity specificity traditional analysis methods, hinder application clinical use. Therefore, it is urgent to develop effective standardized for isolating detecting EVs. Microfluidics technology a powerful rapidly developing that has been introduced solution above bottlenecks. It holds advantages high integration, short time, consumption samples reagents. this review, we summarize techniques alongside microfluidic-based methodologies detection We emphasize distinct microfluidic enhancing capture efficiency precise targeting extracellular (EVs). also explore its analytical role targeted detection. Furthermore, review highlights transformative impact on analysis, achieve automated high-throughput samples.

Language: Английский

Citations

6