Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Plasma-derived extracellular vesicles (EVs) are promising sources of biomarkers. It is still a challenge to isolate EVs from small amount human plasma for downstream proteomic analysis. The isolation process hindered by contamination with high-abundance blood proteins and lipoprotein particles, which adversely impact analyses. Moreover, although EV immune-isolation via magnetic beads often integrates flow sorting Western blotting (WB), it lacks compatibility nanoparticle tracking analysis (NTA) To address these issues, we have developed functional affinity bead, EVlent (Extracellular Vesicles isoLated Efficiently, Naturally, Totally), enabling the rapid efficient plasma. By optimizing quantities used, characterized isolated through WB, NTA, transmission electron microscopy (TEM), showing successful Proteomic identified over 2000 15,000 peptides 100 μL nearly 1000 trace samples as 5 μL. Additionally, this method significantly reduced contaminants, including lipoproteins, compared ultracentrifugation. Finally, applied strategy healthy individuals those Parkinson's disease, identifying four potential biomarkers that provide guidance clinical diagnosis.
Язык: Английский
Процитировано
1
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 1, 2025
Extracellular particles (EPs), including extracellular vesicles (EVs) and non-vesicular (NVEPs), are multimolecular biomaterials released by cells that play a crucial role in intercellular communication. Recently, new subtypes of EPs associated with central nervous system (CNS), such as exophers supermeres have been identified. These provide perspectives for understanding the pathological progression CNS disorders confer potential diagnostic value liquid biopsies neurodegenerative diseases (NDs). Moreover, emerged promising drug delivery vehicles targeted platforms CNS-specific therapies. In this review, we delineate landscape EP their roles pathophysiology diseases. We also review recent advances EP-based diagnosis NDs highlight importance analytical single-particle resolution exploitation biomarkers. Furthermore, summarize application engineered EVs treatment outline underexplored NVEPs novel therapeutic agents.
Язык: Английский
Процитировано
1
HardwareX, Год журнала: 2023, Номер 16, С. e00493 - e00493
Опубликована: Ноя. 12, 2023
Although microparticles are frequently used in chemistry and biology, their effectiveness largely depends on the homogeneity of particle size distribution. Microfluidic devices to separate purify particles based have been developed, but many require expensive cleanroom manufacturing processes. A cost-effective, passive microfluidic separator is presented, capable efficiently sorting purifying spanning range 15 µm 40 µm. Fabricated from Polymethyl Methacrylate (PMMA) substrates using laser ablation, this device circumvents need for facilities. Prior fabrication, rigorous optimization device's design was carried out through computational simulations conducted COMSOL Multiphysics. To gauge its performance, chitosan were employed as a test case. The results notably promising, achieving precision 96.14 %. This quantitative metric underscores size-based separation. low-cost accessible offers pragmatic solution laboratories researchers seeking precise control over sizes, without constraints environments. innovation not only mitigates limitations tied traditional cleanroom-based fabrication also widens horizons various applications within realms biology.
Язык: Английский
Процитировано
16
Pharmaceutics, Год журнала: 2023, Номер 15(1), С. 282 - 282
Опубликована: Янв. 14, 2023
Extracellular vesicles (EVs) are lipid bound particles derived from their original cells, which play critical roles in intercellular communication through cargoes, including protein, lipids, and nucleic acids. According to biogenesis release pathway, EVs can be divided into three categories: apoptotic (ApoVs), microvesicles (MVs), small (sEVs). Recently, the role of oral disease has received close attention. In this review, main characteristics described, classification, biogenesis, biomarkers, components. Moreover, therapeutic mechanism tissue regeneration is discussed. We further summarize current status pulp/periodontal discuss potential mechanisms. The pulp periodontal might involve promotion immunomodulatory capabilities. Furthermore, we highlight challenges translational use EVs. This review would provide valuable insights strategies dental regeneration.
Язык: Английский
Процитировано
14
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13302 - 13302
Опубликована: Дек. 11, 2024
This review aims to address the significant challenges of treating central nervous system (CNS) disorders such as neurodegenerative diseases, strokes, spinal cord injuries, and brain tumors. These are difficult manage due complexity disease mechanisms protective blood–brain barrier (BBB), which restricts drug delivery. Recent advancements in nanoparticle (NP) technologies offer promising solutions, with potential applications delivery, neuroprotection, neuroregeneration. By examining current research, we explore how NPs can cross BBB, deliver medications directly targeted CNS regions, enhance both diagnostics treatment. Key NP strategies, passive targeting, receptor-mediated transport, stimuli-responsive systems, demonstrate encouraging results. Studies show that may improve minimize side effects, increase therapeutic effectiveness models Alzheimer’s, Parkinson’s, stroke, glioblastoma. thus represent a approach for disorder management, combining delivery diagnostic capabilities enable more precise effective treatments could significantly benefit patient outcomes.
Язык: Английский
Процитировано
6
Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 155938 - 155938
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2144 - 2144
Опубликована: Фев. 10, 2024
The members of the Flaviviridae family are becoming an emerging threat for public health, causing increasing number infections each year and requiring effective treatment. consequences these can be severe include liver inflammation with subsequent carcinogenesis, endothelial damage hemorrhage, neuroinflammation, and, in some cases, death. mechanisms pathogenesis being actively investigated, but there still many gaps their understanding. Extracellular vesicles may play important roles mechanisms, therefore, this topic deserves detailed research. Recent data have revealed involvement extracellular steps such as transmission, immune evasion, inflammation, which is critical disease establishment. This review covers recent papers on includes examples clinical applications accumulated data.
Язык: Английский
Процитировано
3
Опубликована: Март 13, 2024
Extracellular vesicles (EVs) hold enormous potential in the diagnosis and treatment of diseases, benefit from their roles intercellular signaling, as well plentiful presence stability body fluids. However, conventional EV isolation methods are labor-intensive, harvest EVs with low purity compromised recovery. In addition, drawbacks, such limited sensitivity specificity traditional analysis hinder application clinical use. Therefore, it is urgent to develop effective standardized for isolating detecting EVs. Microfluidics technology a powerful rapidly developing that has been introduced solution above bottlenecks. It holds advantages high integration, short time, consumption samples reagents. this review, we summarize techniques alongside microfluidic-based methodologies detection We emphasize separation microfluidic enhancing capture efficiency precise targeting, while summarizing its analytical role targeted discussing significance tumor microenvironment, providing direction achieving automated high-throughput samples.
Язык: Английский
Процитировано
3
RSC Advances, Год журнала: 2025, Номер 15(1), С. 167 - 198
Опубликована: Янв. 1, 2025
Microfluidic chips exhibit unique advantages in both economy and rapidity, particularly for the separation detection of biomolecules.
Язык: Английский
Процитировано
0
Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 12
Опубликована: Янв. 22, 2025
Introduction Extracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods differentiating normal and tumor-associated EVs (TAEs) required. Methods This study used quantifiable antibody-DNA conjugate-assisted quantitative combined proximity ligation technology to detect TAEs. The conjugate contained one antibody associated three oligonucleotides signal amplification. in the can recognize surface antigens of Simultaneously, DNA is attached surfaces TAEs holds amplification post, converting protein identities detection, even at molecular level. Results These findings revealed that be quantitatively detected using DNA-mediated polymerase chain reaction (qPCR). Antibody-DNA conjugates were epithelial cell adhesion molecule (EpCAM) antigen on TAE quantify qPCR analysis. Discussion method proposed a new detection approach TAEs, which aim identify specific EV-associated markers diagnostic therapeutic, this could inspire idea diseases.
Язык: Английский
Процитировано
0