PubMed, Год журнала: 2024, Номер 38(1), С. 99 - 106
Опубликована: Янв. 15, 2024
To summarize the progress of roles and mechanisms various types stem cell-based treatments their combination therapies in both animal studies clinical trials lymphedema.
Язык: Английский
International Journal of Biological Sciences, Год журнала: 2024, Номер 20(5), С. 1778 - 1795
Опубликована: Янв. 1, 2024
Skin tissue, composed of epidermis, dermis, and subcutaneous is the largest organ human body.It serves as a protective barrier against pathogens physical trauma plays crucial role in maintaining homeostasis.Skin diseases, such psoriasis, dermatitis, vitiligo, are prevalent can seriously impact quality patient life.Exosomes lipid bilayer vesicles derived from multiple cells with conserved biomarkers important mediators intercellular communication.Exosomes skin cells, blood, stem main types exosomes that involved modulating microenvironment.The dysregulation exosome occurrence transmission, well alterations their cargoes, complex pathogenesis inflammatory autoimmune diseases.Therefore, promising diagnostic therapeutic targets for diseases.Importantly, exogenous exosomes, or play improving environment repairing damaged tissues by carrying various specific active substances involving variety pathways.In domain clinical practice, have garnered attention prospective agents including psoriasis vitiligo.Furthermore, investigations substantiated regenerative efficacy cell-derived repair.In this review, we mainly summarize latest studies about mechanisms applications dermatology, atopic systemic lupus erythematosus, sclerosis, diabetic wound healing, hypertrophic scar keloid, aging.This will provide novel perspective diagnosis treatment dermatosis.
Язык: Английский
Процитировано
21
Biomaterials Research, Год журнала: 2024, Номер 28
Опубликована: Янв. 1, 2024
Random flap grafting is a routine procedure used in plastic and reconstructive surgery to repair reconstruct large tissue defects. Flap necrosis primarily caused by ischemia–reperfusion injury inadequate blood supply the distal flap. Ischemia–reperfusion leads production of excessive reactive oxygen species, creating pathological microenvironment that impairs cellular function angiogenesis. In this study, we developed remodeling self-healing hydrogel [laminarin–chitosan-based hydrogel-loaded extracellular vesicles ceria nanozymes (LCH@EVs&CNZs)] improve synergistically promote regeneration survival. The natural (LCH) was created oxidation laminarin carboxymethylated chitosan via Schiff base reaction. We loaded with CNZs EVs. are class nanomaterials enzymatic activity known for their strong scavenging capacity thus alleviating oxidative stress. EVs cell-secreted vesicular structures containing thousands bioactive substances can cell proliferation, migration, differentiation, constructed LCH@EVs&CNZs demonstrated robust excess thereby conferring protection stress environments. Moreover, these constructs notably enhance migration Our results demonstrate effectively remodel skin marked This approach introduces new therapeutic strategy combining microenvironmental EV therapy, which holds promise promoting
Язык: Английский
Процитировано
9
Genes, Год журнала: 2023, Номер 14(8), С. 1516 - 1516
Опубликована: Июль 25, 2023
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are biologically active substances secreted by MSCs into the matrix that play an immunomodulatory role in skin damage repair. To investigate mechanism of MSC-EVs reducing inflammation, promoting angiogenesis, proliferation and migration epithelial cells fibroblasts, remodeling during wound healing, we focused on effects EVs multiple cell types at various stages injury. A literature review was conducted to explore related research influence involved healing. show a strong regulatory ability immune regulation including macrophages, neutrophils, T cells, other tissue remodeling, such as keratinocytes, endothelial healing vitro vivo experiments, which substantially promoted understanding field trauma medicine. have potential applications combating poor Elucidating action wound-healing process would greatly advance therapeutic
Язык: Английский
Процитировано
16
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 4357 - 4375
Опубликована: Май 1, 2024
Abstract: Wound healing is a sophisticated and orderly process of cellular interactions in which the body restores tissue architecture functionality following injury. Healing chronic diabetic wounds difficult due to impaired blood circulation, reduced immune response, disrupted repair mechanisms, are often associated with diabetes. Stem cell-derived extracellular vesicles (SC-EVs) hold regenerative potential, encapsulating diverse cargo proteins, RNAs, cytokines, presenting safe, bioactivity, less ethical issues than other treatments. SC-EVs orchestrate multiple processes by modulating communication, increasing angiogenesis, promoting recruitment differentiation progenitor cells, thereby potentiating reparative milieu for wound healing. Therefore, this review investigated effects mechanisms EVs from various stem cells healing, as well their limitations challenges. Continued exploration has potential revolutionize care. Keywords: diabetes, EVs, ncRNAs, regeneration
Язык: Английский
Процитировано
5
Current Opinion in Biotechnology, Год журнала: 2024, Номер 85, С. 103067 - 103067
Опубликована: Янв. 25, 2024
Язык: Английский
Процитировано
4
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Май 1, 2024
Abstract Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component stem cell potency, overcome drawbacks cell-free therapeutic agent play an important role in treating various diseases. However, EVs derived from two-dimensional (2D) planar culture SCs low yield face challenges large-scale production, which hinders clinical translation EVs. Three-dimensional (3D) culture, given its ability to more realistically simulate vivo environment, can not only expand large quantities, but also improve activity EVs, changing content improving effects. In this review, we briefly describe advantages EV-related applications, provide overview 3D finally focus on specific future perspectives different SCs. Graphical
Язык: Английский
Процитировано
4
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Июнь 26, 2024
Abstract Background The use of stem cell-derived exosomes (Exos) as therapeutic vehicles is receiving increasing attention. Exosome administration has several advantages over cell transplantation, thus making promising candidates for large-scale clinical implementation and commercialization. However, exosome extraction purification efficiencies are relatively low, heterogeneity high due to differences in culture conditions viability. Therefore, this study, we investigated a priming procedure enhance the production effects from human umbilical cord mesenchymal cells (hucMSCs). After preconditioning hucMSCs with agonists/inhibitors that target Wnt/β-catenin pathway, assessed both efficacy optimized context diabetic wound healing, hoping provide safer, more stable effective option application. Results Wnt signalling pathway agonist CHIR99021 increased by 1.5-fold without causing obvious changes characteristics or size particles. Further studies showed promoted facilitating exocytosis. This process was partly mediated SNAP25. To further explore whether changed cargo loaded into its effects, performed proteomic transcriptomic analyses primed control hucMSCs. results significantly upregulated expression proteins associated migration healing. Animal experiments confirmed that, compared hucMSC-derived exosomes, CHIR99021-pretreated (CHIR-Exos) accelerated healing mice, enhanced local collagen deposition, angiogenesis, reduced chronic inflammation. Subsequent vitro CHIR-Exos migration, inhibiting oxidative stress-induced apoptosis, preventing cycle arrest. Conclusions secretion hucMSCs, which Notably, treatment also exosomal levels resulting acceleration All these suggested pretreatment not only but improved efficacy, providing Graphical
Язык: Английский
Процитировано
4
Journal of Oral Biosciences, Год журнала: 2025, Номер unknown, С. 100621 - 100621
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Regenerative Therapy, Год журнала: 2025, Номер 29, С. 303 - 318
Опубликована: Апрель 5, 2025
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Апрель 24, 2025
Extracellular vesicles (EVs) derived from human organoids are phospholipid bilayer-bound nanoparticles that carry therapeutic cargo. However, the low yield of EVs remains a critical bottleneck for clinical translation. Vertical-Wheel bioreactors (VWBRs), with unique design features, facilitate scalable production secreted by blood vessel (BVOs) under controlled shear stress, using aggregate- and microcarrier-based culture systems. Human induced pluripotent stem cell-derived BVOs cultured as aggregates or on Synthemax II microcarriers within VWBRs (40 80 rpm) were compared to static controls. The characterized metabolite profiling, flow cytometry, gene expression EV biogenesis markers. nanoparticle tracking analysis, electron microscopy, Western blotting. Lipidomics provided insights into lipid composition, while functional assays assessed impact in D-galactose-induced senescence model. VWBR cultures showed more aerobic metabolism higher genes control. different conditions comparable size, but yields significantly microcarrier dynamic than aggregates. Lipidomic profiling revealed minimal variation (< 0.36%) total content; however, distinct differences identified chain lengths saturation levels, affecting key pathways such sphingolipid neurotrophin signaling. BVO demonstrated abilities reducing oxidative stress increasing cell proliferation vitro. differentiated (in particular 40 produce 2-3 fold (per mL) bio manufactured have exosomal characteristics cargo, showing properties vitro assays. This innovative approach establishes platform producing defined profiles potential, paving way future vivo studies.
Язык: Английский
Процитировано
0