Intracellular Delivery Enabled by Squeezing Mechanoporation
Small Methods,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 13, 2025
Abstract
Squeeze
mechanoporation,
as
an
emerging
method,
plays
important
role
in
intracellular
delivery.
It
brings
new
opportunities
to
cutting‐edge
fields
such
cell
therapy,
gene
editing,
and
vaccine
production,
it
promises
revolutionize
traditional
drug
delivery
treatment
paradigms.
By
leveraging
the
viscoelastic
properties
of
cells,
this
technique
induces
deformation
under
external
force,
creating
transient
micropores
membranes
for
efficient
high‐throughput
diverse
exogenous
substances,
nucleic
acids,
antibodies,
nanomaterials,
drugs.
This
review
comprehensively
summarizes
current
advances
mechanical
squeezing‐mediated
delivery,
delving
deeply
into
its
fundamental
principles,
unique
advantages,
latest
applications,
optimization
strategies,
existing
challenges,
corresponding
solutions,
future
development
directions.
With
aim
highlighting
immense
potential
promising
prospects
these
techniques
field
biomanufacturing
therapy.
Язык: Английский
Engineering a Microfluidic Platform to Cryopreserve Stem Cells: A DMSO‐Free Sustainable Approach
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(29)
Опубликована: Авг. 17, 2024
Abstract
Human
adipose‐derived
stem
cells
(hASCs)
are
cryopreserved
traditionally
using
dimethyl
sulfoxide
(DMSO)
as
the
cryoprotectant
agent.
DMSO
penetrates
cell
membranes
and
prevents
cellular
damage
during
cryopreservation.
However,
is
not
inert
to
cells,
inducing
cytotoxic
effects
by
causing
mitochondrial
dysfunction,
reduced
proliferation,
impaired
hASCs
transplantation.
Additionally,
large‐scale
production
of
contamination
can
adversely
impact
environment.
A
sustainable,
green
alternative
trehalose,
a
natural
disaccharide
agent
that
does
pose
any
risk
cytotoxicity.
permeability
trehalose
less
compared
DMSO.
Here,
microfluidic
chip
developed
for
intracellular
delivery
in
hASCs.
The
designed
mechanoporation,
which
creates
transient
pores
mechanical
deformation.
Mechanoporation
allows
sparingly
permeable
be
internalized
within
cytosol.
amount
delivered
intracellularly
quantified
optimized
based
on
compatibility
functionality.
Furthermore,
whole‐transcriptome
sequencing
confirms
than
1%
all
target
genes
display
at
least
twofold
change
expression
when
passed
through
untreated
cells.
Overall,
results
confirm
feasibility
effectiveness
this
DMSO‐free
cryopreservation
Язык: Английский
An Antifreezing Scaffold‐Based Cryopreservation Platform of Stem Cells for Convenient Application in Wound Repair
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
14(4)
Опубликована: Дек. 26, 2024
Efficient
cryopreservation
of
stem
cells
is
crucial
to
fabricating
off-the-shelf
cell
products
for
tissue
engineering
and
regeneration
medicine.
However,
it
remains
challenging
due
utilization
toxic
cryoprotectants
reducing
ice-related
cryodamages
during
freeze-thaw
cycle,
stringent
post-thaw
washing
process,
further
integration
with
scaffolds
form
constructs
downstream
applications.
Herein,
a
novel
platform
based
on
an
antifreezing
polyvinylpyrrolidone/gellan
gum/gelatin
(PGG)
scaffold
together
reported
L-proline
assisted
pre-dehydration
strategy.
Results
show
that
this
capable
inhibiting
extra-/intracellular
ice,
thus
can
achieve
high
cryoprotection
efficacy
(≈95%)
without
using
any
eliminate
traditional
process.
Meanwhile,
the
post-thawed
maintain
their
proliferation,
differentiation,
paracrine
functionalities.
More
importantly,
biocompatibility
three
dimensional
structure
PGG
scaffold,
cell-laden
be
directly
used
as
wound
repair
by
mitigating
inflammation
promoting
collagen
deposition
at
regenerating
sites.
This
present
work
demonstrates
feasibility
scaffold-based
cells,
which
may
advance
clinical
translation.
Язык: Английский