Advanced Fiber Materials, Год журнала: 2025, Номер unknown
Опубликована: Июнь 3, 2025
Язык: Английский
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141250 - 141250
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
2
Advanced Materials, Год журнала: 2025, Номер unknown
Опубликована: Янв. 28, 2025
Abstract Assembling natural proteins into large, strong, bone‐mimetic scaffolds for repairing bone defects in large‐animal load‐bearing sites remain elusive. Here this challenge is tackled by assembling pure silk fibroin (SF) 3D with cortical‐bone‐like lamellae, superior strength, and biodegradability through freeze‐casting. The unique lamellae promote the attachment, migration, proliferation of tissue‐regenerative cells (e.g., mesenchymal stem [MSCs] human umbilical vein endothelial cells) around them, are capable developing vitro cortical‐bone organoids a high number MSC‐derived osteoblasts. High‐SF‐content lamellar scaffolds, regardless MSC inoculation, regenerated more than non‐lamellar or low‐SF‐content scaffolds. They accelerated neovascularization transforming macrophages from M1 to M2 phenotype, promoting regeneration repair large segmental (LSBD) minipigs within three months, even without growth factor supplements. can be further enhanced controlling orientation lamella parallel long axis during implantation. This work demonstrates power oriented bone‐like protein LSBD animal models.
Язык: Английский
Процитировано
1
Chinese Medical Journal, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Abstract The high failure rates in clinical drug development based on animal models highlight the urgent need for more representative human biomedical research. In response to this demand, organoids and organ chips were integrated greater physiological relevance dynamic, controlled experimental conditions. This innovative platform—the organoids-on-a-chip technology—shows great promise disease modeling, discovery, personalized medicine, attracting interest from researchers, clinicians, regulatory authorities, industry stakeholders. review traces evolution organoids-on-a-chip, driven by necessity advanced biological models. We summarize applications of simulating pathological phenotypes therapeutic evaluation technology. section highlights how integrating technologies chips, such as microfluidic systems, mechanical stimulation, sensor integration, optimizes organoid cell types, spatial structure, functions, thereby expanding their applications. conclude addressing current challenges offering insights into prospects. advancement is poised enhance fidelity, standardization, scalability. Furthermore, integration cutting-edge interdisciplinary collaborations will be crucial progression
Язык: Английский
Процитировано
1
Clinical and Translational Medicine, Год журнала: 2025, Номер 15(3)
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
1
Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 599 - 626
Опубликована: Янв. 1, 2025
Silk protein, as a natural polymer material with unique structures and properties, exhibits tremendous potential in the biomedical field. Given limited production restricted properties of silk proteins, molecular biotechnology has been extensively applied protein genetic engineering to produce novel proteins specific properties. This review outlines roles major model organisms, such silkworms spiders, production, provides detailed introduction applications gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, synthetic biology techniques engineering. By analyzing factors influencing expression, this further elaborates on innovative drug delivery systems, tissue regenerative medicine skin, bone, cartilage, vascular repair), well antibacterial immune strategies. Notably, modified expressed by demonstrate significant advantages enhancing bioavailability promoting cell proliferation differentiation. In conclusion, engineering, through continuous innovations biotechnology, provided an effective pathway for high-performance materials. The extensive these field have not only expanded functionality but also offered new approaches address medical challenges. future, development will rely interdisciplinary integration promote in-depth research expansion industrial proteins.
Язык: Английский
Процитировано
0
Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials, Год журнала: 2025, Номер 164, С. 106929 - 106929
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 20, 2025
Uncontrolled deep bleeding, commonly encountered in surgical procedures, combat injuries, and trauma, poses a significant threat to patient survival recovery. The development of effective hemostatic agents capable precisely targeting trauma sites tissues rapidly halt bleeding remains considerable challenge. Drawing inspiration from the natural cascade, we present platelet-like microspheres composed silk fibroin (SF) thrombus-targeting peptides, engineered mimic platelets for rapid hemostasis vivo. These peptide/SF microspheres, formulated using freezing self-assembly technology, closely resemble terms size, shape, zeta potential. Moreover, they exhibit favorable cytocompatibility, hemocompatibility, anti-cell adhesion. Assessment fibrin polymerization revealed that these possessed enzymatic physiological functions, similar activated platelets, facilitating platelet adhesion, binding, wound-triggered hemostasis. Notably, target site vivo within 5 min, with minimal dispersion elsewhere, persisting after blood clot formation. Furthermore, metabolic kinetics, 71% degradation occurring one-day post-subcutaneous injection. Histological assessment well-preserved organ structures inflammatory responses at 14 d post-injection, supporting their long-term biocompatibility. Importantly, can be injected targeted into damaged vessels, selectively binding forming clots 2 resulting 74% reduction volume compared SF alone. Therefore, injectable SF-based emerge as promising candidates future tissue injuries.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142941 - 142941
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Materials & Design, Год журнала: 2025, Номер unknown, С. 114026 - 114026
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Applied Materials Today, Год журнала: 2025, Номер 44, С. 102775 - 102775
Опубликована: Май 12, 2025
Язык: Английский
Процитировано
0