Multifunctional Hydrogel Hybrid‐Gated Organic Photoelectrochemical Transistor for Biosensing

Advanced Functional Materials, Год журнала: 2022, Номер 32(26)

Опубликована: Март 29, 2022

Abstract Advanced organic bioelectronics enable smooth fusion between modern electronics and biological systems for better physiological monitoring pathological examinations. Photon‐regulated are especially desirable due to the non‐contact impact, remote‐control, even self‐powered operation. However, few studies have addressed advanced photon‐enabled photoelectrochemical transistor (OPECT) biosensors capable of operation at zero gate bias. Here, on basis a hydrogel/graphene oxide hybrid (denoted as HGH), multifunctional HGH‐gated OPECT biosensor is presented, which exemplified by Ca 2+ ‐triggered gelation CdS quantum dot (QD) photoelectrode linking with sandwich immunoassay toward human IgG model target. Gelation HGH QD electrode can not only inhibit interfacial mass transfer gate/electrolyte interface, but also significantly block light absorption QDs, leading corresponding change channel currents device. At bias, this exhibits high gain in response good analytical performance detection limit 50 fg mL –1 . Given numerous intelligent hydrogel materials their potential interactions light, work unveils general platform developing new class hydrogel‐gated beyond.

Язык: Английский

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Recent advances in heterogeneous single-atom nanomaterials: From engineered metal-support interaction to applications in sensors

Coordination Chemistry Reviews, Год журнала: 2022, Номер 478, С. 214976 - 214976

Опубликована: Дек. 15, 2022

Язык: Английский

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An alternative to MINFLUX that enables nanometer resolution in a confocal microscope

Light Science & Applications, Год журнала: 2022, Номер 11(1)

Опубликована: Июнь 30, 2022

Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale beyond ensemble averaging. Examples include single-molecule tracking super-resolution imaging by localization microscopy. Among numerous methods available, MINFLUX outstands achieving a ~10-fold improvement in resolution over wide-field camera-based approaches, reaching molecular scale moderate photon counts. Widespread application related has been hindered technical complexity setups. Here, we present RASTMIN, method based on raster scanning light pattern comprising minimum intensity. RASTMIN delivers ~1-2 nm precision with usual fluorophores easily implementable standard confocal microscope few modifications. We demonstrate performance molecules DNA origami structures.

Язык: Английский

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ZIF-8 and benzimidazole co-modified h-BN for enhancing anti-corrosion performance of epoxy coatings

Progress in Organic Coatings, Год журнала: 2023, Номер 183, С. 107808 - 107808

Опубликована: Июль 17, 2023

Язык: Английский

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Graphene-Based Photodynamic Therapy and Overcoming Cancer Resistance Mechanisms: A Comprehensive Review

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 5637 - 5680

Опубликована: Июнь 1, 2024

Abstract: Photodynamic therapy (PDT) is a non-invasive that has made significant progress in treating different diseases, including cancer, by utilizing new nanotechnology products such as graphene and its derivatives. Graphene-based materials have large surface area photothermal effects thereby making them suitable candidates for PDT or photo-active drug carriers. The remarkable photophysical properties of derivates facilitate the efficient generation reactive oxygen species (ROS) upon light irradiation, which destroys cancer cells. Surface functionalization can also enhance their biocompatibility anticancer activity. paper delves into distinct roles played graphene-based photosensitizers (PS) carriers while at same time considers how these could be used to circumvent resistance. This will provide readers with an extensive discussion various pathways contributing inefficiency. Consequently, this comprehensive review underscores vital derivatives may play emerging strategies treatment other medical purposes. With better comprehension current state research existing challenges, integration holds great promise developing targeted, effective, personalized treatments. Keywords: quantum dots, oxide, research, photosensitizers, delivery

Язык: Английский

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Graphene Energy Transfer for Single‐Molecule Biophysics, Biosensing, and Super‐Resolution Microscopy

Advanced Materials, Год журнала: 2021, Номер 33(24)

Опубликована: Май 3, 2021

Abstract Graphene is considered a game‐changing material, especially for its mechanical and electrical properties. This work exploits that graphene almost transparent but quenches fluorescence in range up to ≈40 nm. as broadband unbleachable energy‐transfer acceptor without labeling, used precisely determine the height of molecules with respect graphene, visualize dynamics DNA nanostructures, orientation Förster‐type resonance energy transfer (FRET) pairs. Using origami nanopositioners, biosensing, single‐molecule tracking, PAINT super‐resolution <3 nm z ‐resolution are demonstrated. The examples shows potential graphene‐on‐glass coverslips versatile platform biophysics, microscopy.

Язык: Английский

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Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(4)

Опубликована: Янв. 18, 2023

Fluorescence correlation spectroscopy is a versatile tool for studying fast conformational changes of biomolecules especially when combined with Förster resonance energy transfer (FRET). Despite the many methods available identifying structural dynamics in FRET experiments, determination forward and backward transition rate constants thereby also equilibrium constant difficult two intensity levels are involved. Here, we combine analysis fluorescence lifetime information by including only subset photons autocorrelation based on their arrival time respect to excitation pulse (microtime). By fitting amplitude as function microtime gate, from fluorescence-intensity level systems corresponding obtained. This shrinking-gate (sg-FCS) approach demonstrated using simulations DNA origami-based model system experiments immobilized freely diffusing molecules. We further show that sg-FCS can distinguish photophysics dynamic even if dark quencher, this case graphene, Finally, unravel mechanism FRET-based membrane charge sensor indicating broad potential method. With sg-FCS, present an algorithm does not require prior knowledge therefore easily implemented carried out time-correlated single-photon data.

Язык: Английский

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Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy

Light Science & Applications, Год журнала: 2023, Номер 12(1)

Опубликована: Март 10, 2023

3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques fluorescence imaging. Here, we achieve by combining the 2D localization of pMINFLUX axial information graphene energy transfer (GET) and single-molecule switching DNA-PAINT. We demonstrate <2 nm precision in all 3 dimension reaching below 0.3 nm. In DNA-PAINT measurements, structural features, i.e., individual docking strands at distances nm, are directly resolved on DNA origami structures. GET represent particular synergetic combination for imaging near surface such as cell adhesion membrane complexes each photon used both information. Furthermore, introduce local PAINT (L-PAINT), which imager equipped an additional binding sequence upconcentration improving signal-to-background ratio speed clusters. L-PAINT demonstrated triangular structure 6 side lengths within seconds.

Язык: Английский

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Interplay of graphene–DNA interactions: Unveiling sensing potential of graphene materials

Applied Physics Reviews, Год журнала: 2024, Номер 11(1)

Опубликована: Янв. 26, 2024

Graphene-based materials and DNA probes/nanostructures have emerged as building blocks for constructing powerful biosensors. possess exceptional properties, including two-dimensional atomically flat basal planes biomolecule binding. probes serve excellent selective probes, exhibiting specific recognition capabilities toward diverse target analytes. Meanwhile, nanostructures function placement scaffolds, enabling the precise organization of molecular species at nanoscale positioning complex biomolecular assays. The interplay graphene-based has fostered creation intricate hybrid with user-defined architectures. This advancement resulted in significant progress developing novel biosensors detecting DNA, RNA, small molecules, proteins, well sequencing. Consequently, a profound understanding interactions between is key to these biological devices. In this review, we systematically discussed current comprehension interaction materials, elucidated latest advancements probe–graphene-based Additionally, concisely summarized recent research endeavors involving deposition on explored imminent biosensing applications by seamlessly integrating materials. Finally, delineated primary challenges provided prospective insights into rapidly field. We envision that review will aid researchers gaining deeper insight mechanisms DNA–graphene-based biosensors, designing desired applications.

Язык: Английский

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Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer

Science Advances, Год журнала: 2022, Номер 8(23)

Опубликована: Июнь 8, 2022

Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists remarkable gap between lateral axial which is by factor 2 to 3 worse. One recently developed method close this metal-induced energy transfer (MIET) imaging, achieves an resolution down nanometers. It exploits distance-dependent quenching fluorescence when fluorescent molecule brought metal surface. In present manuscript, we combine extreme MIET imaging with extraordinary single-molecule localization microscopy, particular direct stochastic optical reconstruction ( d STORM). This combination allows us achieve isotropic three-dimensional subcellular structures. Moreover, used spectral demixing implementing dual-color MIET- STORM that image colocalize, three dimensions, different cellular structures simultaneously.

Язык: Английский

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