Metal natural product complex Ru-procyanidins with quadruple enzymatic activity combat infections from drug-resistant bacteria

Acta Pharmaceutica Sinica B, Год журнала: 2024, Номер 14(5), С. 2298 - 2316

Опубликована: Янв. 26, 2024

Bacterial infection hampers wound repair by impeding the healing process. Concurrently, inflammation at site triggers production of reactive oxygen species (ROS), causing oxidative stress and damage to proteins cells. This can lead chronic wounds, posing severe risks. Therefore, eliminating bacterial reducing ROS levels are crucial for effective healing. Nanozymes, possessing enzyme-like catalytic activity, convert endogenous substances into highly toxic substances, such as ROS, combat bacteria biofilms without inducing drug resistance. However, current nanozyme model with single enzyme activity falls short meeting complex requirements antimicrobial therapy. Thus, developing nanozymes multiple enzymatic activities is essential. Herein, we engineered a novel metalloenzyme called Ru-procyanidin nanoparticles (Ru-PC NPs) diverse aid infections. Under acidic conditions, due their glutathione (GSH) depletion peroxidase (POD)-like Ru-PC NPs combined H2O2 exhibit excellent antibacterial effects. in neutral environment, NPs, catalase (CAT) decompose O2, alleviating hypoxia ensuring sufficient supply. Furthermore, possess exceptional antioxidant capacity through superior superoxide dismutase (SOD) effectively scavenging excess nitrogen (RNS) environment. maintains balance system prevents inflammation. also promote polarization macrophages from M1 M2, facilitating More importantly, show good biosafety negligible toxicity. In vivo models have confirmed efficacy inhibiting promoting The focus this work highlights quadruple its potential reduce bacteria-infected

Язык: Английский

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Stabilizer-free MnO2 nanozyme with singlet-oxygen conducted high oxidase-like activity and fast catalysis capability for improving alkaline phosphatase assay

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 404, С. 135280 - 135280

Опубликована: Янв. 13, 2024

Язык: Английский

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p–d Orbital Hybridization-Engineered PdSn Nanozymes for a Sensitive Immunoassay

Nano Letters, Год журнала: 2024, Номер 24(9), С. 2912 - 2920

Опубликована: Фев. 23, 2024

Nanozymes with peroxidase-like activity have been extensively studied for colorimetric biosensing. However, their catalytic and specificity still lag far behind those of natural enzymes, which significantly affects the accuracy sensitivity To address this issue, we design PdSn nanozymes selectively enhanced activity, improves a immunoassay. The is higher than that Pd nanozymes. Theoretical calculations reveal p–d orbital hybridization Sn not only results in an upward shift d-band center to enhance hydrogen peroxide (H2O2) adsorption but also regulates O–O bonding strength H2O2 achieve selective activation. Ultimately, nanozyme-linked immunosorbent assay has successfully developed sensitively accurately detect prostate-specific antigen (PSA), achieving low detection limit 1.696 pg mL–1. This work demonstrates promising approach detecting PSA clinical diagnosis.

Язык: Английский

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Nanozyme‐Enabled Biomedical Diagnosis: Advances, Trends, and Challenges

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Авг. 13, 2024

Abstract As nanoscale materials with the function of catalyzing substrates through enzymatic kinetics, nanozymes are regarded as potential alternatives to natural enzymes. Compared protein‐based enzymes, exhibit attractive characteristics low preparation cost, robust activity, flexible performance adjustment, and versatile functionalization. These advantages endow them wide use from biochemical sensing environmental remediation medical theranostics. Especially in biomedical diagnosis, feature catalytic signal amplification provided by makes emerging labels for detection biomarkers diseases, rapid developments observed recent years. To provide a comprehensive overview progress made this dynamic field, here an diagnosis enabled is provided. This review first summarizes synthesis nanozyme then discusses main strategies applied enhance their activity specificity. Subsequently, representative utilization combined biological elements disease reviewed, including related metabolic, cardiovascular, nervous, digestive diseases well cancers. Finally, some development trends nanozyme‐enabled highlighted, corresponding challenges also pointed out, aiming inspire future efforts further advance promising field.

Язык: Английский

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Rational Design of Nanozymes for Engineered Cascade Catalytic Cancer Therapy

Chemical Reviews, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Nanozymes have shown significant potential in cancer catalytic therapy by strategically catalyzing tumor-associated substances and metabolites into toxic reactive oxygen species (ROS) situ, thereby inducing oxidative stress promoting cell death. However, within the complex tumor microenvironment (TME), rational design of nanozymes factors like activity, reaction substrates, TME itself significantly influence efficiency ROS generation. To address these limitations, recent research has focused on exploring that affect activity developing nanozyme-based cascade systems, which can trigger two or more processes tumors, producing therapeutic achieving efficient stable with minimal side effects. This area remarkable progress. Perspective provides a comprehensive overview nanozymes, covering their classification fundamentals. The regulation nanozyme strategies are discussed detail. Furthermore, representative paradigms for successful construction systems treatment summarized focus revealing underlying mechanisms. Finally, we current challenges future prospects development biomedical applications.

Язык: Английский

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Covalent Coupling-Regulated rGO/VN Nanocomposite Enabling Nitrogen Defects to Remarkably Boost the Peroxidase-Like Catalytic Efficiency for the Ultrasensitive Colorimetric Assay of Uric Acid

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 9, 2025

It remains challenging to rationally design superior nanozymes and understand the underlying mechanism. Herein, a facile covalent coupling-modulated nitrogen defect is reported for significantly boosting peroxidase (POD)-like activity. Vanadium nitride (VN) nanoparticles are grown on graphene oxide (GO) via C–N bonding form VN/rGO nanocomposites by varying with VOx/GO ratio. The initial increasing GO amount enables formation of bond, dramatically POD-like Nevertheless, higher amount, defects decrease due forming mainly V2O3. defect-rich nanocomposite 20 wt % (VG-2) exhibits best catalytic efficiency (Vmax/Km = 0.0187 s–1), which 778-fold than that natural horseradish peroxidase. Theoretical calculations structure characterization reveal rich-N originate from VN binding onto rGO an rich-electron structure, impeding agglomeration, greatly reduces energy barrier rate-determining step reaction. Finally, coupling urate oxidase VG-2 as enzyme cascade, ultrasensitive selective colorimetric detection was developed uric acid (UA), one indicators kidney function or gout attacks, linear ranging 1–100 μM 0.1–2.5 mM limit 0.24 UA (S/N 3). proposed method applicable detecting in human serum samples satisfactorily. This work could inspire more effective insights into designing other robust through variety biochemical analysis biocatalysis applications.

Язык: Английский

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Nanozyme-based wearable biosensors for application in healthcare

iScience, Год журнала: 2025, Номер 28(2), С. 111763 - 111763

Опубликована: Янв. 7, 2025

Recent years have witnessed tremendous advances in wearable sensors, which play an essential role personalized healthcare for their ability real-time sensing and detection of human health information. Nanozymes, capable mimicking the functions natural enzymes addressing limitations, possess unique advantages such as structural stability, low cost, ease mass production, making them particularly beneficial constructing recognition units biosensors. In this review, we aim to delineate latest advancements nanozymes development biosensors, focusing on key developments nanozyme immobilization strategies, technologies, biomedical applications. The review also highlights current challenges future perspectives. Ultimately, it aims provide insights research endeavors rapidly evolving area.

Язык: Английский

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Recent Advancements in the Formulation of Nanomaterials-Based Nanozymes, Their Catalytic Activity, and Biomedical Applications

ACS Applied Bio Materials, Год журнала: 2023, Номер 6(9), С. 3577 - 3599

Опубликована: Авг. 17, 2023

Nanozymes are nanoparticles with intrinsic enzyme-mimicking properties that have become more prevalent because of their ability to outperform conventional enzymes by overcoming drawbacks related stability, cost, and storage. the potential manipulate active sites natural enzymes, which is why they considered promising candidates function as enzyme mimetics. Several microscopy- spectroscopy-based techniques been used for characterization nanozymes. To date, a wide range nanozymes, including catalase, oxidase, peroxidase, superoxide dismutase, designed effectively mimic enzymes. The activity nanozymes can be controlled regulating structural morphological aspects multifaceted benefits, exploited on large scale application in biomedical sector. versatility aids monitoring treating cancer, other neurodegenerative diseases, metabolic disorders. Due compelling advantages significant research advancements made this area. Although act potent mimetics specificities suboptimal, there still room diversification analytical purposes. Designing diverse nanozyme systems sensitive one or substrates through specialized has subject an in-depth study. Hence, we believe stimuli-responsive may open avenues diagnosis treatment fusing catalytic nanomaterial systems.

Язык: Английский

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Reactive oxygen nanobiocatalysts: activity-mechanism disclosures, catalytic center evolutions, and changing states

Chemical Society Reviews, Год журнала: 2023, Номер 52(19), С. 6838 - 6881

Опубликована: Янв. 1, 2023

This review offers a comprehensive and timely summarization of the most recent breakthroughs future trends in creating reactive oxygen nanobiocatalysts, which guides their broad applications diverse biomedical biological fields.

Язык: Английский

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A Self‐Adaptive Pyroptosis Inducer: Optimizing the Catalytic Microenvironment of Nanozymes by Membrane‐Adhered Microbe Enables Potent Cancer Immunotherapy

Advanced Materials, Год журнала: 2023, Номер 36(14)

Опубликована: Дек. 28, 2023

Pyroptosis has garnered increasing attention in cancer immunotherapy. Moreover, plasma membrane damage by reactive oxygen species (ROS) is considered an effective strategy for promoting pyroptosis. However, the current tactics enhancing rupture pyroptosis are limited inherent drawbacks of ROS and immunosuppressive tumor microenvironment. Herein, a self-adaptive inducer (LPZ) designed integrating Lactobacillus rhamnosus GG (LGG) enzyme-like metal-organic framework to achieve potent LPZ can adhere cell membranes through interaction between pili LGG mucin cells. In particular, adaptive formula gradually enhance ability nanozymes produce creating acidic microenvironment anaerobic respiration. These results verify that could generate high levels both on within cells, leading pyroptotic death strong antitumor immunity. Meanwhile, eventually killed this process halt their respiration prevent potential biosafety concerns. Overall, work provides new inspiration design nanocatalytic drugs

Язык: Английский

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