Measurements of molecular size and shape on a chip DOI
Xin Zhu, Timothy J. D. Bennett, Konstantin Zouboulis

и другие.

Science, Год журнала: 2025, Номер 388(6747)

Опубликована: Май 8, 2025

Size and shape are critical discriminators between molecular species states. We describe a microchip-based high-throughput imaging approach offering rapid precise determination of properties under native solution conditions. Our method detects differences in weight across at least three orders magnitude down to two carbon atoms small molecules. quantify the strength interactions more than six affinity constant track reactions real time. Highly parallel measurements on individual molecules serve characterize sample-state heterogeneity highest resolution, predictive input model three-dimensional structure. further leverage method’s structural sensitivity for diagnostics, exploiting ligand-induced conformational changes insulin receptor sense concentration serum subnanoliter subzeptomole scale.

Язык: Английский

Multi-pass, single-molecule nanopore reading of long protein strands DOI Creative Commons
Keisuke Motone,

Daphne Kontogiorgos-Heintz,

Jasmine Wee

и другие.

Nature, Год журнала: 2024, Номер 633(8030), С. 662 - 669

Опубликована: Сен. 11, 2024

Язык: Английский

Процитировано

37

Nanofluidics DOI

Theo Emmerich,

Nathan Ronceray, Kumar Varoon Agrawal

и другие.

Nature Reviews Methods Primers, Год журнала: 2024, Номер 4(1)

Опубликована: Сен. 19, 2024

Процитировано

15

PVDF-based membranes in biotechnology DOI
Antonio Tabernero, Álvaro González‐Garcinuño, Stefano Cardea

и другие.

Separation and Purification Technology, Год журнала: 2025, Номер unknown, С. 132636 - 132636

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Controlled Sensing of User-Defined Aptamer-Based Targets Using Scanning Ionic Conductance Spectroscopy DOI Creative Commons
Helena Miljkovic, Lely Feletti,

Gérard Blanchet

и другие.

ACS Nano, Год журнала: 2025, Номер 19(13), С. 13139 - 13148

Опубликована: Март 31, 2025

Solid-state nanopores offer the possibility of detecting disease biomarkers in early diagnostic applications. Standard approaches harness fingerprinting, where protein targets are bound to DNA carriers and detected free translocation with a solid-state nanopore. However, they suffer from several drawbacks, including uncontrolled fast translocations, which lead low detection accuracy signal-to-noise ratio (SNR). This has hampered their application clinical settings. Here, we propose nanopore-based system capable sensing selected molecules interest biological fluids by harnessing programmable aptamer sequences attached carrier systems that tethered glass surfaces. allows for spatial velocity control over x, y, z directions enables repeated scanning same analyte. The ion conductance spectroscopy (SICS) based approach distinguishes itself standard its ability repeatedly scan molecule target site more than 5 times. We designed multiple binding sites different aptamers increase yield experiment. Our achieves rate up 74%, significantly higher 14% achieved nanopore measurements. strong also increased densities along carrier, thereby paving way multiplexed sensing. offers user-defined programmability sequences, potentially expanding use our sense other biomarkers.

Язык: Английский

Процитировано

0

The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing DOI Creative Commons
Marzia Iarossi, Navneet C. Verma,

Ivy Bhattacharya

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Driven by recent advancements in nanofabrication techniques, single-molecule sensing and manipulations nanofluidic devices are rapidly evolving. These sophisticated biosensors have already had significant impacts on basic research as well applications molecular diagnostics. The nanoscale dimensions of these introduce new physical phenomena confining the biomolecules at least one dimension, creating effects such biopolymer linearization, stretching, separation mass that utilized to enhance biomolecule resolutions. At same time, suppressed diffusional motion allows for better SNR (signal-to-noise ratio) over time. In particular, based nanochannels been established promising technologies linearization ultralong genomic DNA molecules optical genome mapping, opening a window directly observe infer organization. More recently, shown capabilities protein sizing, separation, identification. Consequently, this technology is attracting remarkable interest proteomics. review, we discuss nanochannel-based technologies, focusing their characterization wide range biomolecules.

Язык: Английский

Процитировано

0

Measurements of molecular size and shape on a chip DOI
Xin Zhu, Timothy J. D. Bennett, Konstantin Zouboulis

и другие.

Science, Год журнала: 2025, Номер 388(6747)

Опубликована: Май 8, 2025

Size and shape are critical discriminators between molecular species states. We describe a microchip-based high-throughput imaging approach offering rapid precise determination of properties under native solution conditions. Our method detects differences in weight across at least three orders magnitude down to two carbon atoms small molecules. quantify the strength interactions more than six affinity constant track reactions real time. Highly parallel measurements on individual molecules serve characterize sample-state heterogeneity highest resolution, predictive input model three-dimensional structure. further leverage method’s structural sensitivity for diagnostics, exploiting ligand-induced conformational changes insulin receptor sense concentration serum subnanoliter subzeptomole scale.

Язык: Английский

Процитировано

0