Multi-pass, single-molecule nanopore reading of long protein strands
Nature,
Год журнала:
2024,
Номер
633(8030), С. 662 - 669
Опубликована: Сен. 11, 2024
Язык: Английский
Nanofluidics
Nature Reviews Methods Primers,
Год журнала:
2024,
Номер
4(1)
Опубликована: Сен. 19, 2024
PVDF-based membranes in biotechnology
Separation and Purification Technology,
Год журнала:
2025,
Номер
unknown, С. 132636 - 132636
Опубликована: Март 1, 2025
Язык: Английский
Controlled Sensing of User-Defined Aptamer-Based Targets Using Scanning Ionic Conductance Spectroscopy
ACS Nano,
Год журнала:
2025,
Номер
19(13), С. 13139 - 13148
Опубликована: Март 31, 2025
Solid-state
nanopores
offer
the
possibility
of
detecting
disease
biomarkers
in
early
diagnostic
applications.
Standard
approaches
harness
fingerprinting,
where
protein
targets
are
bound
to
DNA
carriers
and
detected
free
translocation
with
a
solid-state
nanopore.
However,
they
suffer
from
several
drawbacks,
including
uncontrolled
fast
translocations,
which
lead
low
detection
accuracy
signal-to-noise
ratio
(SNR).
This
has
hampered
their
application
clinical
settings.
Here,
we
propose
nanopore-based
system
capable
sensing
selected
molecules
interest
biological
fluids
by
harnessing
programmable
aptamer
sequences
attached
carrier
systems
that
tethered
glass
surfaces.
allows
for
spatial
velocity
control
over
x,
y,
z
directions
enables
repeated
scanning
same
analyte.
The
ion
conductance
spectroscopy
(SICS)
based
approach
distinguishes
itself
standard
its
ability
repeatedly
scan
molecule
target
site
more
than
5
times.
We
designed
multiple
binding
sites
different
aptamers
increase
yield
experiment.
Our
achieves
rate
up
74%,
significantly
higher
14%
achieved
nanopore
measurements.
strong
also
increased
densities
along
carrier,
thereby
paving
way
multiplexed
sensing.
offers
user-defined
programmability
sequences,
potentially
expanding
use
our
sense
other
biomarkers.
Язык: Английский
The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 17, 2025
Driven
by
recent
advancements
in
nanofabrication
techniques,
single-molecule
sensing
and
manipulations
nanofluidic
devices
are
rapidly
evolving.
These
sophisticated
biosensors
have
already
had
significant
impacts
on
basic
research
as
well
applications
molecular
diagnostics.
The
nanoscale
dimensions
of
these
introduce
new
physical
phenomena
confining
the
biomolecules
at
least
one
dimension,
creating
effects
such
biopolymer
linearization,
stretching,
separation
mass
that
utilized
to
enhance
biomolecule
resolutions.
At
same
time,
suppressed
diffusional
motion
allows
for
better
SNR
(signal-to-noise
ratio)
over
time.
In
particular,
based
nanochannels
been
established
promising
technologies
linearization
ultralong
genomic
DNA
molecules
optical
genome
mapping,
opening
a
window
directly
observe
infer
organization.
More
recently,
shown
capabilities
protein
sizing,
separation,
identification.
Consequently,
this
technology
is
attracting
remarkable
interest
proteomics.
review,
we
discuss
nanochannel-based
technologies,
focusing
their
characterization
wide
range
biomolecules.
Язык: Английский
Measurements of molecular size and shape on a chip
Science,
Год журнала:
2025,
Номер
388(6747)
Опубликована: Май 8, 2025
Size
and
shape
are
critical
discriminators
between
molecular
species
states.
We
describe
a
microchip-based
high-throughput
imaging
approach
offering
rapid
precise
determination
of
properties
under
native
solution
conditions.
Our
method
detects
differences
in
weight
across
at
least
three
orders
magnitude
down
to
two
carbon
atoms
small
molecules.
quantify
the
strength
interactions
more
than
six
affinity
constant
track
reactions
real
time.
Highly
parallel
measurements
on
individual
molecules
serve
characterize
sample-state
heterogeneity
highest
resolution,
predictive
input
model
three-dimensional
structure.
further
leverage
method’s
structural
sensitivity
for
diagnostics,
exploiting
ligand-induced
conformational
changes
insulin
receptor
sense
concentration
serum
subnanoliter
subzeptomole
scale.
Язык: Английский