The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing DOI Creative Commons
Marzia Iarossi, Navneet C. Verma,

Ivy Bhattacharya

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Driven by recent advancements in nanofabrication techniques, single-molecule sensing and manipulations nanofluidic devices are rapidly evolving. These sophisticated biosensors have already had significant impacts on basic research as well applications molecular diagnostics. The nanoscale dimensions of these introduce new physical phenomena confining the biomolecules at least one dimension, creating effects such biopolymer linearization, stretching, separation mass that utilized to enhance biomolecule resolutions. At same time, suppressed diffusional motion allows for better SNR (signal-to-noise ratio) over time. In particular, based nanochannels been established promising technologies linearization ultralong genomic DNA molecules optical genome mapping, opening a window directly observe infer organization. More recently, shown capabilities protein sizing, separation, identification. Consequently, this technology is attracting remarkable interest proteomics. review, we discuss nanochannel-based technologies, focusing their characterization wide range biomolecules.

Language: Английский

Multi-pass, single-molecule nanopore reading of long protein strands DOI Creative Commons
Keisuke Motone,

Daphne Kontogiorgos-Heintz,

Jasmine Wee

et al.

Nature, Journal Year: 2024, Volume and Issue: 633(8030), P. 662 - 669

Published: Sept. 11, 2024

Language: Английский

Citations

37
Nanofluidics DOI

Theo Emmerich,

Nathan Ronceray, Kumar Varoon Agrawal

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: Sept. 19, 2024

Citations

14
PVDF-based membranes in biotechnology DOI
Antonio Tabernero, Álvaro González‐Garcinuño, Stefano Cardea

et al.

Separation and Purification Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132636 - 132636

Published: March 1, 2025

Language: Английский

Citations

0
Controlled Sensing of User-Defined Aptamer-Based Targets Using Scanning Ionic Conductance Spectroscopy DOI Creative Commons
Helena Miljkovic, Lely Feletti,

Gérard Blanchet

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: 19(13), P. 13139 - 13148

Published: March 31, 2025

Solid-state nanopores offer the possibility of detecting disease biomarkers in early diagnostic applications. Standard approaches harness fingerprinting, where protein targets are bound to DNA carriers and detected free translocation with a solid-state nanopore. However, they suffer from several drawbacks, including uncontrolled fast translocations, which lead low detection accuracy signal-to-noise ratio (SNR). This has hampered their application clinical settings. Here, we propose nanopore-based system capable sensing selected molecules interest biological fluids by harnessing programmable aptamer sequences attached carrier systems that tethered glass surfaces. allows for spatial velocity control over x, y, z directions enables repeated scanning same analyte. The ion conductance spectroscopy (SICS) based approach distinguishes itself standard its ability repeatedly scan molecule target site more than 5 times. We designed multiple binding sites different aptamers increase yield experiment. Our achieves rate up 74%, significantly higher 14% achieved nanopore measurements. strong also increased densities along carrier, thereby paving way multiplexed sensing. offers user-defined programmability sequences, potentially expanding use our sense other biomarkers.

Language: Английский

Citations

0
The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing DOI Creative Commons
Marzia Iarossi, Navneet C. Verma,

Ivy Bhattacharya

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Driven by recent advancements in nanofabrication techniques, single-molecule sensing and manipulations nanofluidic devices are rapidly evolving. These sophisticated biosensors have already had significant impacts on basic research as well applications molecular diagnostics. The nanoscale dimensions of these introduce new physical phenomena confining the biomolecules at least one dimension, creating effects such biopolymer linearization, stretching, separation mass that utilized to enhance biomolecule resolutions. At same time, suppressed diffusional motion allows for better SNR (signal-to-noise ratio) over time. In particular, based nanochannels been established promising technologies linearization ultralong genomic DNA molecules optical genome mapping, opening a window directly observe infer organization. More recently, shown capabilities protein sizing, separation, identification. Consequently, this technology is attracting remarkable interest proteomics. review, we discuss nanochannel-based technologies, focusing their characterization wide range biomolecules.

Language: Английский

Citations

0