MOLECULARLY IMPRINTED POLYMERS FOR PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Journal of Pharmaceutical and Biomedical Analysis Open, Год журнала: 2024, Номер 4, С. 100038 - 100038

Опубликована: Авг. 23, 2024

Язык: Английский

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An Advanced Multivalent Ligand‐Decorated Microsphere Enrichment System Efficiently Captures Circulating Tumor Cells In Vivo

Small, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Abstract Capturing circulating tumor cells (CTCs) in vivo from the bloodstream lessens metastasis and recurrence risks. However, absence of CTC receptors due to epithelial‐mesenchymal transition (EMT), limited binding capacity a single ligand, complexity blood flow environment significantly reduce efficiency capture vivo. Herein, multivalent ligand‐decorated microsphere enrichment system (MLMES) is crafted that incorporates column replete with an immunosorbent precisely recognizes binds stably expressed cluster differentiation 44 (CD44) glucose transporter protein 1 (GLUT1) present on exterior CTCs. As peripheral flows through column, CTCs are efficiently captured, achieving rate up 64.2%, highest reported date. Moreover, MLMES demonstrates excellent biocompatibility, broad‐spectrum capture, storage stability. Importantly, it eliminates substantial quantity blood, reducing risk metastasis. This breakthrough method has broad clinical application potential preventing recurrence, bringing new possibilities for improving cancer treatment.

Язык: Английский

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Innovative theranostic hydrogels for targeted gastrointestinal cancer treatment

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 27, 2024

Gastrointestinal tumors are the main causes of death among patients. These mainly diagnosed in advanced stages and their response to therapy is unfavorable. In spite development conventional therapeutics including surgery, chemotherapy, radiotherapy immunotherapy, treatment these still challenging. As a result, new based on (nano)biotechnology have been introduced. Hydrogels polymeric 3D networks capable absorbing water swell with favorable biocompatibility. application hydrogels different human diseases, wide cancer has improved because potential drug gene delivery, boosting chemotherapy immunotherapy as well vaccines. The current review focuses role gastrointestinal tumors. provide delivery drugs (both natural or synthetic compounds co-delivery) along delivery. Along stimulate phototherapy (photothermal photodynamic therapy) suppression Besides, ability for induction immune-related cells such dendritic can boost immunotherapy. For more specific therapy, stimuli-responsive types thermo- pH-sensitive self-healing site Moreover, promising diagnosis, circulating tumor cell isolation detection biomarkers tumors, highlighting importance clinic. Hence, diagnostic therapeutic tools gastrointestimal

Язык: Английский

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Smart polymers advance analytical chemistry

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118198 - 118198

Опубликована: Фев. 1, 2025

Язык: Английский

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Theoretical Calculations in Separation Science for Analytical Chemistry: Applications and Insights

Chemistry - An Asian Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Abstract Separation and enrichment are critical steps in analytical detection, necessitating advanced materials with high selectivity adsorption capacity for target compounds. In order to improve separation efficiency selectivity, computational simulation could elucidate interaction mechanisms analyze potential adsorption/desorption processes, providing a theoretical foundation the optimization design of materials. Recently, has become an indispensable crucial mean science chemistry. Using various software, researchers investigate structures, properties, performance at multiple levels scales. this review, we summarize applications simulations field science, focusing on polar molecules, geometric isomers, enantiomer compounds, post‐translationally modified peptides. These calculation methods include quantum chemistry, molecular docking, dynamics simulations, high‐throughput screening, machine learning. Finally, discuss current challenges breakthroughs simulation, aiming offer valuable insights dedicated material development, applications.

Язык: Английский

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Proximity-induced amplification cascade for accurate and sensitive colorimetric detection of tumor-derived exosomes

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137832 - 137832

Опубликована: Апрель 1, 2025

Язык: Английский

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Fusion of Molecular and Mechanical Phenotypes Enables High-Purity and Low-Loss Reacquisition of Viable CTCs for Transcriptome Analysis

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

The high-purity and low-loss reacquisition of viable circulating tumor cells (CTCs) is crucial for enabling downstream omics analysis CTCs currently represents key challenges limiting their application in clinical diagnosis pathological research. Given the limitations traditional methods that rely solely on a molecular or mechanical phenotype acquisition, this study introduces an innovative approach fuses inherent phenotypes into new phenotype, thereby achieving preconcentration CTCs. Specifically, blood are immunomodified using calcium carbonate microspheres (CCMSs) conjugated with antibodies, transforming (membrane protein expression) additional (increased size reduced deformability). This transformation enhances distinctions between white cells, single-cell trapping array chip. Since CCMSs can be reversibly eliminated under weak acid, captured nondestructively reacquired 93.10% microliter-scale solution, allowing subsequent analysis. In breast cancer mouse model, counts transcriptome provide valuable insights assessing occurrence progression.

Язык: Английский

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Integrating Metabolic Oligosaccharide Engineering and SPAAC Click Chemistry for Constructing Fibrinolytic Cell Surfaces

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(28), С. 35874 - 35886

Опубликована: Июль 2, 2024

To effectively solve the problem of significant loss transplanted cells caused by thrombosis during cell transplantation, this study simulates human fibrinolytic system and combines metabolic oligosaccharide engineering with strain-promoted azide–alkyne cycloaddition (SPAAC) click chemistry to construct a surface activity. First, copolymer (POL) oligoethylene glycol methacrylate (OEGMA) 6-amino-2-(2-methylamido)hexanoic acid (Lys) was synthesized reversible addition–fragmentation chain transfer (RAFT) copolymerization, dibenzocyclooctyne (DBCO) functional group introduced into side through an active ester reaction, resulting in functionalized DBCO-PEG4-POL ε-lysine ligands. Then, azide groups were onto HeLa model engineering, further specifically modified via SPAAC "click" reaction. In vitro investigations revealed that compared unmodified cells, not only resist adsorption nonspecific proteins such as fibrinogen serum albumin but also selectively bind plasminogen plasma while maintaining good viability proliferative More importantly, upon activation adsorbed plasmin, exhibited remarkable activity capable promptly dissolving primary thrombus formed on their surfaces. This research provides novel approach for constructing transplantable offers new perspective addressing thrombosis.

Язык: Английский

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Engineering Peptide-Based Molecular Baits for Targeted Fishing and Protein Profiling of Exosomes as a Liquid Biopsy for Gastrointestinal Adenocarcinoma

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 741 - 748

Опубликована: Дек. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Язык: Английский

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Photoelectrochemical aptasensing and fluorescence imaging co-joint detecting MCF-7 cells in whole blood via an inertial separation microfluidic chip

Talanta, Год журнала: 2024, Номер 286, С. 127488 - 127488

Опубликована: Дек. 26, 2024

Язык: Английский

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