Talanta, Journal Year: 2024, Volume and Issue: 286, P. 127488 - 127488
Published: Dec. 26, 2024
Language: Английский
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 7, 2025
Abstract Capturing circulating tumor cells (CTCs) in vivo from the bloodstream lessens metastasis and recurrence risks. However, absence of CTC receptors due to epithelial‐mesenchymal transition (EMT), limited binding capacity a single ligand, complexity blood flow environment significantly reduce efficiency capture vivo. Herein, multivalent ligand‐decorated microsphere enrichment system (MLMES) is crafted that incorporates column replete with an immunosorbent precisely recognizes binds stably expressed cluster differentiation 44 (CD44) glucose transporter protein 1 (GLUT1) present on exterior CTCs. As peripheral flows through column, CTCs are efficiently captured, achieving rate up 64.2%, highest reported date. Moreover, MLMES demonstrates excellent biocompatibility, broad‐spectrum capture, storage stability. Importantly, it eliminates substantial quantity blood, reducing risk metastasis. This breakthrough method has broad clinical application potential preventing recurrence, bringing new possibilities for improving cancer treatment.
Language: Английский
Citations
1
Journal of Pharmaceutical and Biomedical Analysis Open, Journal Year: 2024, Volume and Issue: 4, P. 100038 - 100038
Published: Aug. 23, 2024
Language: Английский
Citations
7
Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown
Published: April 9, 2025
Abstract Separation and enrichment are critical steps in analytical detection, necessitating advanced materials with high selectivity adsorption capacity for target compounds. In order to improve separation efficiency selectivity, computational simulation could elucidate interaction mechanisms analyze potential adsorption/desorption processes, providing a theoretical foundation the optimization design of materials. Recently, has become an indispensable crucial mean science chemistry. Using various software, researchers investigate structures, properties, performance at multiple levels scales. this review, we summarize applications simulations field science, focusing on polar molecules, geometric isomers, enantiomer compounds, post‐translationally modified peptides. These calculation methods include quantum chemistry, molecular docking, dynamics simulations, high‐throughput screening, machine learning. Finally, discuss current challenges breakthroughs simulation, aiming offer valuable insights dedicated material development, applications.
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137832 - 137832
Published: April 1, 2025
Language: Английский
Citations
0
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: Oct. 27, 2024
Gastrointestinal tumors are the main causes of death among patients. These mainly diagnosed in advanced stages and their response to therapy is unfavorable. In spite development conventional therapeutics including surgery, chemotherapy, radiotherapy immunotherapy, treatment these still challenging. As a result, new based on (nano)biotechnology have been introduced. Hydrogels polymeric 3D networks capable absorbing water swell with favorable biocompatibility. application hydrogels different human diseases, wide cancer has improved because potential drug gene delivery, boosting chemotherapy immunotherapy as well vaccines. The current review focuses role gastrointestinal tumors. provide delivery drugs (both natural or synthetic compounds co-delivery) along delivery. Along stimulate phototherapy (photothermal photodynamic therapy) suppression Besides, ability for induction immune-related cells such dendritic can boost immunotherapy. For more specific therapy, stimuli-responsive types thermo- pH-sensitive self-healing site Moreover, promising diagnosis, circulating tumor cell isolation detection biomarkers tumors, highlighting importance clinic. Hence, diagnostic therapeutic tools gastrointestimal
Language: Английский
Citations
3
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118198 - 118198
Published: Feb. 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 741 - 748
Published: Dec. 30, 2024
High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.
Language: Английский
Citations
1
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(28), P. 35874 - 35886
Published: July 2, 2024
To effectively solve the problem of significant loss transplanted cells caused by thrombosis during cell transplantation, this study simulates human fibrinolytic system and combines metabolic oligosaccharide engineering with strain-promoted azide–alkyne cycloaddition (SPAAC) click chemistry to construct a surface activity. First, copolymer (POL) oligoethylene glycol methacrylate (OEGMA) 6-amino-2-(2-methylamido)hexanoic acid (Lys) was synthesized reversible addition–fragmentation chain transfer (RAFT) copolymerization, dibenzocyclooctyne (DBCO) functional group introduced into side through an active ester reaction, resulting in functionalized DBCO-PEG4-POL ε-lysine ligands. Then, azide groups were onto HeLa model engineering, further specifically modified via SPAAC "click" reaction. In vitro investigations revealed that compared unmodified cells, not only resist adsorption nonspecific proteins such as fibrinogen serum albumin but also selectively bind plasminogen plasma while maintaining good viability proliferative More importantly, upon activation adsorbed plasmin, exhibited remarkable activity capable promptly dissolving primary thrombus formed on their surfaces. This research provides novel approach for constructing transplantable offers new perspective addressing thrombosis.
Language: Английский
Citations
1
Talanta, Journal Year: 2024, Volume and Issue: 286, P. 127488 - 127488
Published: Dec. 26, 2024
Language: Английский
Citations
0