Animals and zoonoses., Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Materials Today Bio, Год журнала: 2025, Номер 30, С. 101443 - 101443
Опубликована: Янв. 5, 2025
Glioblastoma (GBM) is the most prevalent primary malignant brain tumor, characterized by a high mortality rate and poor prognosis. The blood-brain barrier (BBB) blood-tumor (BTB) present significant obstacles to efficacy of tumor-targeted pharmacotherapy, thereby impeding therapeutic potential numerous candidate drugs. Targeting delivery adequate doses drug across BBB treat GBM has become prominent research area in recent years. This emphasis driven exploration evaluation diverse technologies for with some already undergoing clinical trials. review provides thorough overview advancements challenges targeted treatment. It specifically emphasizes systemic administration strategies assess their limitations Furthermore, this highlights promising future directions development intelligent systems aimed at overcoming current enhancing against GBM. These not only support foundational on but also offer methodological approaches applications.
Язык: Английский
Процитировано
5
International Journal of Molecular Medicine, Год журнала: 2025, Номер 55(3)
Опубликована: Янв. 8, 2025
Exosomes are integral to the pathophysiology of osteoarthritis (OA) due their roles in mediating intercellular communication and regulating inflammatory processes. transport bioactive molecules, such as proteins, lipids nucleic acids, which can influence chondrocyte behavior joint homeostasis. Given properties regeneration ability target damaged tissues, exosomes represent a promising therapeutic avenue for OA treatment. have potential promoting cartilage repair, reducing inflammation improving overall function. However, several challenges remain, including need standardized isolation characterization methods, variability exosomal content, regulatory hurdles. The present review aims provide comprehensive overview current understanding exosome mechanisms potential, while also addressing ongoing faced translating these findings into clinical practice. By consolidating existing research, pave way future studies aimed at optimizing exosome‑based therapies effective management.
Язык: Английский
Процитировано
2
Cancers, Год журнала: 2025, Номер 17(6), С. 940 - 940
Опубликована: Март 10, 2025
Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, positioning them transformative tools for cancer diagnostics therapeutics. Recent advancements exosome engineering, artificial intelligence (AI)-driven analytics, isolation technologies are breaking barriers scalability, reproducibility, clinical application. Bioengineered exosomes being leveraged CRISPR-Cas9 delivery, while AI models enhancing biomarker discovery liquid biopsy accuracy. Despite these advancements, key obstacles heterogeneity populations lack standardized protocols persist. This review synthesizes pioneering research on biology, molecular translation, emphasizing dual roles both mediators tumor progression intervention. It also explores emerging areas, including microbiome–exosome interactions integration machine learning exosome-based medicine. By bridging innovation with translational strategies, this work charts a forward-looking path integrating into next-generation care, setting it apart comprehensive guide overcoming technological hurdles rapidly evolving field.
Язык: Английский
Процитировано
2
Neuroglia, Год журнала: 2025, Номер 6(1), С. 3 - 3
Опубликована: Янв. 4, 2025
Background/Objectives: Glioblastoma (GBM), a highly aggressive grade IV astrocytoma, poses major therapeutic challenge due to the resistance of cancer stem cells (CSCs) existing within its cell population conventional therapies. Recently, we reported that RNA interference targeting CSC protection mechanism significantly improved efficacy. However, challenges remain, including limited transfection efficiency in neural and difficulty crossing blood–brain barrier (BBB). Methods: In this study, investigated potential exosome-mediated delivery cargo GBM by engineering exosomes carry green fluorescent protein (GFP) expressing brain-homing peptide (BHP) on their surface, which has high affinity cells. Results: We found BHP-modified doubled GFP efficacy from 20% 40%, outperforming traditional methods like lipofection vitro. vivo, demonstrated an ability cross BBB targeted brain regions following intranasal subcutaneous administration. Conclusions: These results underscore engineered for efficient enhance against tumors suggest novel avenues delivering biomolecules treatment neurological disorders.
Язык: Английский
Процитировано
1
Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер 106, С. 106696 - 106696
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
1
Cancer Gene Therapy, Год журнала: 2025, Номер unknown
Опубликована: Фев. 26, 2025
Язык: Английский
Процитировано
1
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Abstract Exosomes, small extracellular vesicles with lipid bilayer membranes, play a crucial role in cellular communication and can transfer diverse biological cargo, including proteins, lipids, nucleic acids, from donor to recipient cells. Exosomes possess immunological properties, such as antigen delivery immune activation, along excellent drug capabilities, making them promising candidates for vaccine development. For different diseases, exosome‐based vaccines be designed therapeutic or prophylactic by leveraging immunity humoral immunity. With the emergence of precision medicine, personalized demonstrate exceptional potential. This review systematically introduces sources, biogenesis mechanisms, components exosomes describes their regulatory roles system. Subsequently, preparation, administration, therapy are discussed. Finally, applications clinical trials fields anti‐infection anti‐tumor therapies particularly highlighted, an analysis potential challenges future
Язык: Английский
Процитировано
1
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Апрель 17, 2025
Small extracellular vesicles (sEVs) are membrane-bound with a size of less than 200 nm, released by cells. Due to their relatively small molecular weight and ability participate in intercellular communication, sEVs can serve not only as carriers biomarkers for disease diagnosis but also effective drug delivery agents. Furthermore, these involved regulating the onset progression various diseases, reflecting physiological functional states This paper introduces classification vesicles, focus on extraction identification significant role repair, diagnosis, communication. Additionally, addresses engineering modification provide reference enhanced understanding application.
Язык: Английский
Процитировано
1
Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 12, 2024
Язык: Английский
Процитировано
9
Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113614 - 113614
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
1