Sequential Angiogenic-Osteogenic Coupling via a Spatiotemporally Graded Hydrogel Enables Vascularized Bone Organoids for Critical-Sized Calvarial Defect Reconstruction
Xu Lou,
Fuxiao Wang,
Xia Lv
и другие.
Composites Part B Engineering,
Год журнала:
2025,
Номер
unknown, С. 112553 - 112553
Опубликована: Апрель 1, 2025
Язык: Английский
Intelligent Manufacturing for Osteoarthritis Organoids
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 26, 2025
ABSTRACT
Osteoarthritis
(OA)
is
the
most
prevalent
degenerative
joint
disease
worldwide,
imposing
a
substantial
global
burden.
However,
its
pathogenesis
remains
incompletely
understood,
and
effective
treatment
strategies
are
still
lacking.
Organoid
technology,
in
which
stem
cells
or
progenitor
self‐organise
into
miniature
tissue
structures
under
three‐dimensional
(3D)
culture
conditions,
provides
promising
vitro
platform
for
simulating
pathological
microenvironment
of
OA.
This
approach
can
be
employed
to
investigate
mechanisms,
carry
out
high‐throughput
drug
screening
facilitate
personalised
therapies.
review
summarises
structure,
OA
manifestations,
thereby
establishing
context
application
organoid
technology.
It
then
examines
components
arthrosis
system,
specifically
addressing
cartilage,
subchondral
bone,
synovium,
skeletal
muscle
ligament
organoids.
Furthermore,
it
details
various
constructing
organoids,
including
considerations
cell
selection,
classification
fabrication
techniques.
Notably,
this
introduces
concept
intelligent
manufacturing
organoids
by
incorporating
emerging
engineering
technologies
such
as
artificial
intelligence
(AI)
process,
forming
an
innovative
software
hardware
cluster.
Lastly,
discusses
challenges
currently
facing
highlights
future
directions
rapidly
evolving
field.
By
offering
comprehensive
overview
state‐of‐the‐art
methodologies
challenges,
anticipates
that
intelligent,
automated
will
expedite
fundamental
research,
discovery
translational
applications
orthopaedic
Язык: Английский
Organoid Vascularization: Strategies and Applications
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 26, 2025
Abstract
Organoids
provide
3D
structures
that
replicate
native
tissues
in
biomedical
research.
The
development
of
vascular
networks
within
organoids
enables
oxygen
and
nutrient
delivery
while
facilitating
metabolic
waste
removal,
which
supports
organoid
growth
maturation.
Recent
studies
demonstrate
vascularized
models
offer
insights
into
tissue
interactions
promote
regeneration.
However,
the
current
limitations
establishing
functional
affect
growth,
viability,
clinical
translation
potential.
This
review
examines
organoids,
including
mechanisms
angiogenesis
vasculogenesis,
construction
strategies,
applications.
approaches
are
categorized
vivo
vitro
methods,
with
analysis
their
specific
advantages
limitations.
also
discusses
emerging
techniques
such
as
bioprinting
gene
editing
for
improving
vascularization
integration
organoid‐based
therapies.
Current
developments
indicate
potential
applications
modeling
human
diseases
developing
therapeutic
contributing
to
advances
translational
Язык: Английский