Dynamic Hydrogels with Tunable Mechanics for 3D Organoid Derivation DOI

Xueyong Xie,

Xuewen Chen, Jian Zhou

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: May 28, 2025

Abstract The mechanical properties of the hydrogel play a pivotal role in governing formation and development 3D organoids vitro. However, commonly employed natural hydrogels, such as Matrigel other extracellular matrix (ECM)‐derived products, are characterized by ill‐defined complex compositions, resulting non‐tunable properties. This limitation poses challenges controlling organoids' developmental trajectory morphology. Although numerous synthetic hydrogels with well‐defined chemical structures have recently been adopted to study modulating stiffness, advanced research emphasizes importance dynamic cues, stiffness softening viscoelasticity, for optimal organoid derivation. These cues essential mimicking physiological states during their growth. Despite potential, concept is often used interchangeably, systematic review lacking clarify this ambiguity. Furthermore, mechanisms through which regulate not thoroughly reported. endeavors summarize categorize reveal effects mechanics on Additionally, prospects derivation deliberated promote more rational design guiding propelling technology biomedicine.

Language: Английский

Intelligent Manufacturing for Osteoarthritis Organoids DOI Creative Commons
Xin Lyu, Jian Wang, Jiacan Su

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

ABSTRACT Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide, imposing a substantial global burden. However, its pathogenesis remains incompletely understood, and effective treatment strategies are still lacking. Organoid technology, in which stem cells or progenitor self‐organise into miniature tissue structures under three‐dimensional (3D) culture conditions, provides promising vitro platform for simulating pathological microenvironment of OA. This approach can be employed to investigate mechanisms, carry out high‐throughput drug screening facilitate personalised therapies. review summarises structure, OA manifestations, thereby establishing context application organoid technology. It then examines components arthrosis system, specifically addressing cartilage, subchondral bone, synovium, skeletal muscle ligament organoids. Furthermore, it details various constructing organoids, including considerations cell selection, classification fabrication techniques. Notably, this introduces concept intelligent manufacturing organoids by incorporating emerging engineering technologies such as artificial intelligence (AI) process, forming an innovative software hardware cluster. Lastly, discusses challenges currently facing highlights future directions rapidly evolving field. By offering comprehensive overview state‐of‐the‐art methodologies challenges, anticipates that intelligent, automated will expedite fundamental research, discovery translational applications orthopaedic

Language: Английский

Citations

1
Sequential Angiogenic-Osteogenic Coupling via a Spatiotemporally Graded Hydrogel Enables Vascularized Bone Organoids for Critical-Sized Calvarial Defect Reconstruction DOI

Xu Lou,

Fuxiao Wang,

Xia Lv

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112553 - 112553

Published: April 1, 2025

Language: Английский

Citations

0
Organoid Vascularization: Strategies and Applications DOI
Qianmin Gao, Jian Wang, Hao Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Organoids provide 3D structures that replicate native tissues in biomedical research. The development of vascular networks within organoids enables oxygen and nutrient delivery while facilitating metabolic waste removal, which supports organoid growth maturation. Recent studies demonstrate vascularized models offer insights into tissue interactions promote regeneration. However, the current limitations establishing functional affect growth, viability, clinical translation potential. This review examines organoids, including mechanisms angiogenesis vasculogenesis, construction strategies, applications. approaches are categorized vivo vitro methods, with analysis their specific advantages limitations. also discusses emerging techniques such as bioprinting gene editing for improving vascularization integration organoid‐based therapies. Current developments indicate potential applications modeling human diseases developing therapeutic contributing to advances translational

Language: Английский

Citations

0
A 3D Co‐Culture System Inspired by Fracture Healing Cell Interactions for Bone Tissue Engineering DOI
Jicenyuan Wu, Yuxuan Wang, Liang Wang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

Abstract Peri‐bone fibroblasts play a crucial role in regulating bone regeneration during early fracture healing. Inspired by the synergy between osteoblasts and at sites, biomimetic three‐dimensional (3D) indirect co‐culture system is developed, comprising 3D scaffold co‐cultured cells. To mimic cellular interactions healing zone, features an inner–outer ring structure with communication channels that support cell co‐culture. This setup provides culture environment resembling vivo extracellular matrix, enhancing intercellular signaling while minimizing risks of direct contact. Mechanically tunable bioinks are formulated incorporating hyaluronic acid methacrylate (HAMA) hydrogel into gelatin methacryloyl (GelMA) to construct scaffold. The optimal ratio established vitro, where found regulate osteogenic differentiation marrow mesenchymal stem cells (BMSCs) via zinc ion transport mechanisms. In validations conducted, including ectopic formation nude mice rat cranial defect tooth extraction socket models. enhances osteogenesis promoting functional fibroblast–osteoblast interactions, offering novel platform for studies promising strategy clinical applications.

Language: Английский

Citations

0
Dynamic Hydrogels with Tunable Mechanics for 3D Organoid Derivation DOI

Xueyong Xie,

Xuewen Chen, Jian Zhou

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: May 28, 2025

Abstract The mechanical properties of the hydrogel play a pivotal role in governing formation and development 3D organoids vitro. However, commonly employed natural hydrogels, such as Matrigel other extracellular matrix (ECM)‐derived products, are characterized by ill‐defined complex compositions, resulting non‐tunable properties. This limitation poses challenges controlling organoids' developmental trajectory morphology. Although numerous synthetic hydrogels with well‐defined chemical structures have recently been adopted to study modulating stiffness, advanced research emphasizes importance dynamic cues, stiffness softening viscoelasticity, for optimal organoid derivation. These cues essential mimicking physiological states during their growth. Despite potential, concept is often used interchangeably, systematic review lacking clarify this ambiguity. Furthermore, mechanisms through which regulate not thoroughly reported. endeavors summarize categorize reveal effects mechanics on Additionally, prospects derivation deliberated promote more rational design guiding propelling technology biomedicine.

Language: Английский

Citations

0