Critical Reviews in Analytical Chemistry, Год журнала: 2023, Номер 54(7), С. 2599 - 2617

Опубликована: Март 20, 2023

Food safety is one of the greatest public problems occurring around world. Chemical, physical, and microbiological hazards could lead to food problems, which might occur at all stages supply chain. To tackle protect consumer health, specific, accurate, rapid diagnosis techniques meeting various requirements are imperative measures ensure safety. CRISPR-Cas system, a novel emerging technology, effectively repurposed in (bio)sensing has shown tremendous capability develop on-site portable diagnostic methods with high specificity sensitivity. Among numerous existing CRISPR/Cas systems, CRISPR/Cas13a CRISPR/Cas12a extensively employed design biosensors, owing their ability cleave both non-target target sequences. However, limitation hindered its progress. Nowadays, nucleic acid aptamers recognized for high-affinity characteristics analytes incorporated into systems. With benefits reproducibility, durability, portability, facile operation, cost-effectiveness, CRISPR/Cas-based aptasensing approaches an ideal choice fabricating highly specific point-of-need analytical tools enhanced response signals. In current study, we explore some most recent progress CRISPR/Cas-mediated aptasensors detecting risk factors including veterinary drugs, pesticide residues, pathogens, mycotoxins, heavy metals, illegal additives, other contaminants. The nanomaterial engineering support also signified achieve hopeful perspective provide new straightforward test kits toward trace amounts different contaminants encountered samples.

Язык: Английский