The
high
mortality
associated
with
certain
cancers
can
be
attributed
to
the
invasive
nature
of
tumor
cells.
Yet,
complexity
studying
invasion
hinders
our
understanding
how
spreads.
This
work
presents
a
microengineered
three-dimensional
(3D)
in
vitro
model
for
cancer
cell
and
interaction
endothelial
was
generated
by
printing
biomimetic
hydrogel
scaffold
directly
on
chip
using
2-photon
polymerization
that
simulates
brain's
extracellular
matrix.
scaffold's
geometry
specifically
designed
facilitate
growth
continuous
layer
cells
one
side,
while
also
allowing
introduction
other
side.
arrangement
confines
spatially
enables
situ
microscopy
as
they
invade
interact
layer.
We
examined
impact
3D
parameters
hydrogel's
physical
properties
used
patient
derived
glioblastoma
study
their
effect
invasion.
Notably,
tended
infiltrate
faster
when
an
barrier
present.
potential
adjusting
properties,
coupled
capability
real-time
observation
tumor-endothelial
interactions,
offers
platform
tumor–endothelial
interactions.
Gels,
Год журнала:
2024,
Номер
10(4), С. 220 - 220
Опубликована: Март 25, 2024
This
study
explores
the
dynamic
field
of
3D-printed
hydrogels,
emphasizing
advancements
and
challenges
in
customization,
fabrication,
functionalization
for
applications
biomedical
engineering,
soft
robotics,
tissue
engineering.
It
delves
into
significance
tailored
scaffolds
regeneration,
enhancement
bioinks
realistic
replication,
development
bioinspired
actuators.
Additionally,
this
paper
addresses
fabrication
issues
aiming
to
mimic
biological
structures
through
high-resolution,
multimaterial
printing.
In
it
highlights
efforts
create
environments
conducive
cell
migration
functional
development.
research
also
extends
drug
delivery
systems,
focusing
on
controlled
release
biocompatibility,
examines
integration
hydrogels
with
electronic
components
bioelectronic
applications.
The
interdisciplinary
nature
these
a
commitment
overcoming
material
limitations
optimizing
techniques
realize
full
potential
improving
health
well-being.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Окт. 17, 2024
Wound
healing
represents
a
complex
biological
process,
critically
important
in
clinical
practice
due
to
its
direct
implication
patient's
recovery
and
quality
of
life.
Conservative
wound
management
frequently
falls
short
providing
an
ideal
environment
for
the
optimal
tissue
regeneration,
often
resulting
extended
periods
elevated
risk
infection
other
complications.
The
emerging
biomaterials,
particularly
hydrogels,
have
shown
substantial
promise
addressing
these
challenges
by
offering
properties
such
as
biocompatibility,
biodegradability,
ability
cure
environment.
Recent
advancements
highlighted
therapeutic
potential
integrating
cell-derived
conditioned
medium
(CM)
into
hydrogel
matrices.
Cell-derived
CM
rich
array
bioactive
molecules,
demonstrating
significant
efficacy
modulating
cellular
activities
crucial
healing,
including
proliferation,
migration,
angiogenesis.
Advanced Materials Technologies,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Abstract
Although
hydrogels
are
among
the
most
promising
materials
for
a
huge
variety
of
biomimicking
and
tissue
engineering
applications,
conventional
such
as
polydimethylsiloxane
(PDMS)
still
outweigh
in
terms
processability
production
microfluidic
devices.
Hence,
incorporating
hydrogel
components
inside
PDMS‐based
chips
is
approach
to
take
advantage
many
possibilities
utilize
hydrogels,
while
maintaining
standard
properties
devices
mechanical
stability.
Microfluidic
produced
by
soft
lithography
combined
with
high‐resolution
protein‐based
elements
fabricated
two‐photon
polymerization
(2PP).
Those
hybrid
used
distinguish
different
cell
phenotypes
injecting
pancreatic
cancer
cells
device
investigate
interactions
microstructures.
The
Young's
modulus
blocks
printed
at
experimental
conditions
determined
atomic
force
microscopy
measurements.
To
showcase
high
3D
resolution
presented
fabrication
method,
fully
fibrous
meshes
configurations
microchannels.
By
measuring
velocity
circularity
that
pass
through
varying
densities,
impact
on
flow
determined.
Furthermore,
precursor
solution
successfully
removed
immersed
phosphate
buffered
saline.
Polymers,
Год журнала:
2025,
Номер
17(6), С. 765 - 765
Опубликована: Март 14, 2025
The
architectures
of
hydrogels
fabricated
with
stereolithography
(SLA)
3D
printing
systems
have
played
various
roles
in
bioengineering
applications.
Typically,
the
SLA
successively
illuminated
light
to
a
layer
photo-crosslinkable
hydrogel
precursors
for
fabrication
hydrogels.
These
can
be
classified
into
point-scanning
types
and
digital
micromirror
device
(DMD)
types.
form
layers
by
scanning
focused
light,
while
DMD
illuminate
2D
patterns
each
at
once.
Overall,
were
cost-effective
allowed
good
shape
fidelity
uniform
mechanical
properties.
As
result,
constructs
used
regenerate
tissues
develop
lab-on-a-chip
devices
native
tissue-like
models.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 3, 2025
Abstract
Fabricating
hydrogel‐based
channels
with
diameters
below
200
µm
remains
challenging
in
advanced
vitro
modeling
and
tissue
engineering.
To
address
this
challenge,
thermoshrinkable
hydrogels
that
undergo
reversible
isotropic
dimensional
changes
temperature
are
developed.
A
thermoresponsive
polymer
methacrylate
groups
(PNH‐MA)
is
synthesized
from
polyethylene
glycol
(PEG),
N–isopropylacrylamide
(NIPAM),
2‐hydroxyethyl
acrylate
(HEA),
enabling
photo‐cross‐linking
precise
material
tuning.
PNH‐MA
can
shrink
up
to
90%
volume
(50%
diameter)
remain
transparent
allowing
cellular
imaging.
In
a
four‐dimension
(4D)
fabrication
strategy,
seeded
proximal
tubule
epithelial
cells
shrunk
reduce
diameters.
Using
pin
pull‐out
mold
casting,
of
120
410
65
µm,
respectively.
While
needle
injection
for
smaller
than
volumetric
printing
addresses
limitation.
The
shrinkage
properties
enable
leak‐proof
perfusion,
cell
seeding
continuous
unilateral
flow
as
small
100170
µm.
polymers
represent
one
the
few
examples
low‐viscosity
resins
successfully
used
hydrogel
complex
scaffolds.
This
study
highlights
potential
scalable,
high‐precision
tubular
scaffold
modeling.
There
is
a
continued
increase
in
demand
for
novel
bone
grafting
substitutes
to
reduce
reliance
on
and
address
challenges
associated
with
allograft
autograft
grafts.
Current
synthetic
exhibit
low
mechanical
strength
bioactivity,
which
has
inspired
the
development
of
materials.
Accelerating
translation
new
graft
requires
workflows
high-throughput
fabrication
analysis
particle-containing
models.
This
study
utilized
3D
sacrificial
printing
reproducible,
cellular
scaffolds
containing
tricalcium
phosphate
(TCP),
hydroxyapatite
(HA),
or
natural
coral
particles.
High-throughput
included
quantifying
cell
metabolism,
viability,
calcium
consumption,
as
well
nondestructive
collagen
accumulation
destructive
methods
assessing
number
morphological
changes.
Both
particle-
non-particle-containing
inks
sustained
metabolism
decreasing
death
7
days
post-printing.
Collagen
staining,
scanning
electron
microscopy
imaging,
quantification
suggested
that,
under
osteogenic
induction
conditions,
cells
migrated
surface
formed
sheet
collagen-containing
extracellular
matrix,
thereby
indicating
differentiation.
The
workflow
described
herein
enables
creation
vitro
models
nature
substitute
combined
non-destructive
screening
techniques
resulted
reduced
time,
resources,
costs
could
be
applicable
broader
range
types.
Materials,
Год журнала:
2024,
Номер
17(10), С. 2194 - 2194
Опубликована: Май 7, 2024
The
concept
of
ecology,
historically
rooted
in
the
economy
nature,
currently
needs
to
evolve
encompass
intricate
web
interactions
among
humans
and
various
organisms
environment,
which
are
influenced
by
anthropogenic
forces.
In
this
review,
definition
ecology
has
been
adapted
address
dynamic
interplay
energy,
resources,
information
shaping
both
natural
artificial
ecosystems.
Previously,
3D
(and
4D)
printing
technologies
have
presented
as
potential
tools
within
ecological
framework,
promising
a
new
for
nature.
However,
despite
considerable
scientific
discourse
surrounding
printing,
there
remains
significant
gap
research
exploring
between
these
directions.
Therefore,
holistic
review
incorporating
principles
into
practices
is
presented,
emphasizing
environmental
sustainability,
resource
efficiency,
innovation.
Furthermore,
'unecological'
aspects
disadvantages
related
legal
aspects,
intellectual
property,
legislation,
well
societal
impacts,
underlined.
These
ideas
collectively
suggest
roadmap
future
practice.
This
calls
more
comprehensive
understanding
multifaceted
impacts
development
responsible
aligned
with
goals.
Recent
advancements
in
vascular
organoid
(VO)
and
vessel-on-chip
(VoC)
technologies
have
revolutionized
our
approach
to
studying
cardiovascular
diseases
(CVDs),
offering
unprecedented
insights
through
more
physiologically
relevant
models.
VOs
generated
from
human
pluripotent
stem
cells
exhibit
remarkable
self-organization
capabilities,
forming
complex
three-dimensional
structures
that
closely
mimic
blood
vessel
architecture
function,
while
VoCs
engineered
with
groundbreaking
microfluidic
systems
meticulously
recreate
the
physical
functional
attributes
of
vessels.
These
innovative
constructs
serve
as
powerful
tools
for
investigating
development,
disease
progression,
therapeutic
efficacy.
By
enabling
creation
patient-specific
VoCs,
they
pave
way
personalized
medicine
approaches,
allowing
researchers
delve
into
genetic
variations,
intricate
cellular
interactions,
dynamic
processes
exceptional
resolution.
The
synergy
between
cutting-edge
such
single-cell
sequencing
high-resolution
imaging
has
further
amplified
their
potential,
unveiling
novel
mechanisms
underlying
CVDs
identifying
promising
targets.
Herein,
we
summarize
different
types
present
an
extensive
overview
on
generation
applications
CVDs.
We
will
also
highlight
clinical
translational
challenges
future
perspectives
around
VoCs.
Biomaterials Advances,
Год журнала:
2024,
Номер
167, С. 214105 - 214105
Опубликована: Ноя. 5, 2024
Over
the
last
decade,
3D
bioprinting
has
gained
increasing
popularity,
being
a
technique
capable
of
producing
well-defined
tissue-like
structures.
One
its
most
groundbreaking
features
is
ability
to
create
personalized
therapies
tailored
specific
demands
individual
patients.
However,
challenges
including
selection
materials
and
crosslinking
strategies,
still
need
be
addressed
enhance
ink
characteristics
develop
robust
biomaterials.
Herein,
authors
showcase
potential
overcoming
these
challenges,
focusing
on
use
versatile,
fast,
selective
thiol-ene
click
chemistry
formulate
inks
for
bioprinting.
The
exploration
natural
polymers,
specifically
proteins
polysaccharides,
will
discussed
highlighted,
outlining
advantages
disadvantages
this
approach.
Leveraging
advanced
polymers
in
development
printable
bioinks
may
face
current
envisioned
pave
way
towards
innovative
biomaterials
biomedical
applications.
