Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer‐Lipid Hybrid Vesicles
Advanced Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 12, 2025
Abstract
Creating
artificial
cells
with
a
dynamic
cytoskeleton,
akin
to
those
in
living
cells,
is
major
goal
bottom‐up
synthetic
biology.
In
this
study,
we
demonstrate
the
situ
polymerization
of
microtubules
encapsulated
giant
polymer‐lipid
hybrid
vesicles
(GHVs)
composed
1,2‐dioleoyl‐
sn
‐glycero‐3‐phosphocholine
and
an
amphiphilic
block
copolymer.
The
copolymer
comprised
poly(cholesteryl
methacrylate‐
co
‐butyl
methacrylate)
as
hydrophobic
either
poly(6‐O‐methacryloyl‐D‐galactopyranose)
or
poly(carboxyethyl
acrylate)
hydrophilic
extension.
Depending
on
concentrations
guanosine
triphosphate
(GTP)
its
slowly
hydrolyzable
analog,
guanosine‐5′‐[(α,β)‐methyleno]triphosphate
(GMPCPP),
different
microtubule
morphologies
are
observed,
including
networks,
spike
protrusions,
well
membrane‐associated
aggregated
microtubules.
Overall,
work
represents
step
forward
mimicking
cellular
cytoskeletons
uncovering
influence
membrane
composition
morphologies.
Язык: Английский
Cell Membrane Vesicle Camouflaged Artificial Cells
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
Abstract
The
integration
of
artificial
and
mammalian
cells
into
semi‐synthetic
aggregates
remains
a
challenge
in
bottom‐up
synthetic
biology.
Here,
the
fabrication
cell
membrane
vesicles
(CMV)
from
HepG2
their
use
as
coating
for
alginate
microgels
to
produce
camouflaged
(ACs)
is
demonstrated.
These
ACs
are
used
assembly
either
or
aggregates.
In
first
case,
predator‐defendant
liver‐like
investigated,
showing
promising
initial
steps
toward
complex
other
show
enhanced
with
cells.
encapsulation
reactive
oxygen
species
(ROS)
scavenger
enzyme
shows
protection
against
tert
‐butyl
hydroperoxide
terms
viability,
proliferation,
mitochondrial
health
Taken
together,
this
effort
substantial
step
forward
combining
same
aggregate
where
latter
act
support
units.
Язык: Английский
Receptor-Mediated Transmembrane Activation of Protein Folding in Synthetic Cells
Bioconjugate Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 17, 2025
Synthetic
cells
are
a
rapidly
maturing
platform
with
emerging
applications
in
biomedicine
and
biotechnology.
The
specific
novelty
of
this
work
is
that
we
develop
synthetic
respond
to
an
extracellular
stimulus
by
performing
the
folding
encapsulated
polypeptide
into
functional
enzyme.
To
end,
developed
artificial
transmembrane
signaling
receptors.
These
contain
enzyme-responsive
group,
self-immolative
linker
as
mechanism
signal
transduction,
secondary
messenger
molecule
intracellular
activity.
chosen
such
it
initiates
protein
refolding
from
denatured
polypeptide.
Results
study
expand
molecular
toolbox
for
design
life-like,
responsive
behavior.
Язык: Английский
Meta-adaptive biomaterials: multiscale, spatiotemporal organization and actuation in engineered tissues
Gerardo Cedillo-Servin,
Essa A A Al-Jehani,
Tamara Rossy
и другие.
Trends in biotechnology,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 1, 2025
Язык: Английский
Light‐Based Juxtacrine Signaling Between Synthetic Cells
Small Science,
Год журнала:
2024,
Номер
5(1)
Опубликована: Окт. 30, 2024
Cell
signaling
through
direct
physical
cell-cell
contacts
plays
vital
roles
in
biology
during
development,
angiogenesis,
and
immune
response.
Intercellular
communication
mechanisms
between
synthetic
cells
constructed
from
the
bottom
up
are
majorly
reliant
on
diffusible
chemical
signals,
thus
limiting
range
of
responses
receiver
cells.
Engineering
contact-dependent
promises
to
unlock
more
complicated
schemes
with
spatial
responses.
Herein,
a
light-activated
scheme
for
is
designed
demonstrated.
A
split
luminescent
protein
utilized
limit
signal
generation
exclusively
contact
interfaces
cells,
driving
recruitment
photoswitchable
akin
juxtacrine
living
The
modular
design
not
only
demonstrates
but
also
provides
platform
engineering
orthogonal
mechanisms.
Язык: Английский