Light‐Based Juxtacrine Signaling Between Synthetic Cells DOI Creative Commons
Hossein Moghimianavval,

Kyle J. Loi,

Sung‐Won Hwang

et al.

Small Science, Journal Year: 2024, Volume and Issue: 5(1)

Published: Oct. 30, 2024

Cell signaling through direct physical cell-cell contacts plays vital roles in biology during development, angiogenesis, and immune response. Intercellular communication mechanisms between synthetic cells constructed from the bottom up are majorly reliant on diffusible chemical signals, thus limiting range of responses receiver cells. Engineering contact-dependent promises to unlock more complicated schemes with spatial responses. Herein, a light-activated scheme for is designed demonstrated. A split luminescent protein utilized limit signal generation exclusively contact interfaces cells, driving recruitment photoswitchable akin juxtacrine living The modular design not only demonstrates but also provides platform engineering orthogonal mechanisms.

Language: Английский

Cell Membrane Vesicle Camouflaged Artificial Cells DOI Creative Commons
Paula De Dios Andres, Stefan Pendlmayr, Noga Gal

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract The integration of artificial and mammalian cells into semi‐synthetic aggregates remains a challenge in bottom‐up synthetic biology. Here, the fabrication cell membrane vesicles (CMV) from HepG2 their use as coating for alginate microgels to produce camouflaged (ACs) is demonstrated. These ACs are used assembly either or aggregates. In first case, predator‐defendant liver‐like investigated, showing promising initial steps toward complex other show enhanced with cells. encapsulation reactive oxygen species (ROS) scavenger enzyme shows protection against tert ‐butyl hydroperoxide terms viability, proliferation, mitochondrial health Taken together, this effort substantial step forward combining same aggregate where latter act support units.

Language: Английский

Citations

0
Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer‐Lipid Hybrid Vesicles DOI Creative Commons
Paula De Dios Andres, Mousumi Akter,

Cecilie Ryberg

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is major goal bottom‐up synthetic biology. In this study, we demonstrate the situ polymerization of microtubules encapsulated giant polymer‐lipid hybrid vesicles (GHVs) composed 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphocholine and an amphiphilic block copolymer. The copolymer comprised poly(cholesteryl methacrylate‐ co ‐butyl methacrylate) as hydrophobic either poly(6‐O‐methacryloyl‐D‐galactopyranose) or poly(carboxyethyl acrylate) hydrophilic extension. Depending on concentrations guanosine triphosphate (GTP) its slowly hydrolyzable analog, guanosine‐5′‐[(α,β)‐methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including networks, spike protrusions, well membrane‐associated aggregated microtubules. Overall, work represents step forward mimicking cellular cytoskeletons uncovering influence membrane composition morphologies.

Language: Английский

Citations

0
Receptor-Mediated Transmembrane Activation of Protein Folding in Synthetic Cells DOI Creative Commons
Andreas Bøtker Pedersen,

Dante Guldbrandsen Andersen,

Josefine Hammer Jakobsen

et al.

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Synthetic cells are a rapidly maturing platform with emerging applications in biomedicine and biotechnology. The specific novelty of this work is that we develop synthetic respond to an extracellular stimulus by performing the folding encapsulated polypeptide into functional enzyme. To end, developed artificial transmembrane signaling receptors. These contain enzyme-responsive group, self-immolative linker as mechanism signal transduction, secondary messenger molecule intracellular activity. chosen such it initiates protein refolding from denatured polypeptide. Results study expand molecular toolbox for design life-like, responsive behavior.

Language: Английский

Citations

0
Light‐Based Juxtacrine Signaling Between Synthetic Cells DOI Creative Commons
Hossein Moghimianavval,

Kyle J. Loi,

Sung‐Won Hwang

et al.

Small Science, Journal Year: 2024, Volume and Issue: 5(1)

Published: Oct. 30, 2024

Cell signaling through direct physical cell-cell contacts plays vital roles in biology during development, angiogenesis, and immune response. Intercellular communication mechanisms between synthetic cells constructed from the bottom up are majorly reliant on diffusible chemical signals, thus limiting range of responses receiver cells. Engineering contact-dependent promises to unlock more complicated schemes with spatial responses. Herein, a light-activated scheme for is designed demonstrated. A split luminescent protein utilized limit signal generation exclusively contact interfaces cells, driving recruitment photoswitchable akin juxtacrine living The modular design not only demonstrates but also provides platform engineering orthogonal mechanisms.

Language: Английский

Citations

1