Objective:
Escherichia
coli
O157:H7
(
E.
O157:H7)
can
induce
severe
diseases
in
animals
and
humans
that
result
significant
public
health
problems.
Therefore,
the
development
of
rapid
visual
detection
methods
to
diagnose
infections
monitor
its
prevalence
is
critical
for
prevention
control
purposes.
Methods:
A
colorimetric
loop-mediated
isothermal
amplification
(LAMP)
assay
was
utilized
detect
O157:H7.
DNA
extraction
kit
Flinders
Technology
Associates
(FTA)
cards
were
used
extract
nucleic
acid
conjunction
with
LAMP
detection.
Results:
The
method
developed
effectively
distinguished
from
other
pathogens
a
limit
25
CFU/mL
spiked
stool
samples.
In
addition,
these
samples
easily
extracted
transported
an
FTA
card
at
room
temperature.
entire
process
completed
within
35
min
using
simple
constant-temperature
equipment.
Conclusion:
card-based
purification
shown
rapidly
sensitivity
specificity.
This
expected
be
widely
infections,
particularly
resource-limited
settings.
ACS Sensors,
Год журнала:
2023,
Номер
8(12), С. 4420 - 4441
Опубликована: Ноя. 18, 2023
CRISPR/Cas
system
is
becoming
an
increasingly
influential
technology
that
has
been
repositioned
in
nucleic
acid
detection.
A
preamplification
step
usually
required
to
improve
the
sensitivity
of
CRISPR/Cas-based
The
striking
biological
features
CRISPR/Cas,
including
programmability,
high
and
sequence
specificity,
single-base
resolution.
More
strikingly,
target-activated
trans-cleavage
could
act
as
a
biocatalytic
signal
transductor
amplifier,
thereby
empowering
it
potentially
perform
detection
without
step.
reports
such
work
are
on
rise,
which
not
only
scientifically
significant
but
also
promising
for
futuristic
end-user
applications.
This
review
started
with
introduction
methods
acids
diagnostics
(CRISPR-Dx).
Next,
we
objectively
discussed
pros
cons
steps
CRISPR-Dx.
We
then
illustrated
highlighted
recently
developed
strategies
CRISPR/Cas-powered
amplification-free
can
be
realized
through
uses
ultralocalized
reactors,
cascade
reactions,
ultrasensitive
systems,
or
others.
Lastly,
challenges
perspectives
were
proposed.
It
expected
this
makes
researchers
better
understand
current
emerging
field,
provides
insight
designing
novel
CRISPR-Dx
win
practicable
use
near
future.
Biosensors,
Год журнала:
2023,
Номер
13(6), С. 584 - 584
Опубликована: Май 27, 2023
Conventional
diagnostic
techniques
are
based
on
the
utilization
of
analyte
sampling,
sensing
and
signaling
separate
platforms
for
detection
purposes,
which
must
be
integrated
to
a
single
step
procedure
in
point
care
(POC)
testing
devices.
Due
expeditious
nature
microfluidic
platforms,
trend
has
been
shifted
toward
implementation
these
systems
analytes
biochemical,
clinical
food
technology.
Microfluidic
molded
with
substances
such
as
polymers
or
glass
offer
specific
sensitive
infectious
noninfectious
diseases
by
providing
innumerable
benefits,
including
less
cost,
good
biological
affinity,
strong
capillary
action
simple
process
fabrication.
In
case
nanosensors
nucleic
acid
detection,
some
challenges
need
addressed,
cellular
lysis,
isolation
amplification
before
its
detection.
To
avoid
laborious
steps
executing
processes,
advances
have
deployed
this
perspective
on-chip
sample
preparation,
introduction
an
emerging
field
modular
microfluidics
that
multiple
advantages
over
microfluidics.
This
review
emphasizes
significance
technology
non-infectious
diseases.
The
isothermal
conjunction
lateral
flow
assay
greatly
increases
binding
efficiency
nanoparticles
biomolecules
improves
limit
sensitivity.
Most
importantly,
deployment
paper-based
material
made
cellulose
reduces
overall
cost.
discussed
explicating
applications
different
fields.
Next-generation
methods
can
improved
using
CRISPR/Cas
systems.
concludes
comparison
future
prospects
various
systems,
plasma
separation
used
Biosensors,
Год журнала:
2023,
Номер
13(3), С. 410 - 410
Опубликована: Март 21, 2023
Infectious
diseases
contribute
significantly
to
the
global
disease
burden.
Sensitive
and
accurate
screening
methods
are
some
of
most
effective
means
identifying
sources
infection
controlling
infectivity.
Conventional
detecting
strategies
such
as
quantitative
polymerase
chain
reaction
(qPCR),
DNA
sequencing,
mass
spectrometry
typically
require
bulky
equipment
well-trained
personnel.
Therefore,
a
large
population
using
conventional
during
pandemic
periods
often
requires
additional
manpower,
resources,
time,
which
cannot
be
guaranteed
in
resource-limited
settings.
Recently,
emerging
microfluidic
technologies
have
shown
potential
replace
performing
point-of-care
detection
because
they
automated,
miniaturized,
integrated.
By
exploiting
spatial
separation
sites,
platforms
can
enable
multiplex
infectious
reduce
possibility
misdiagnosis
incomplete
diagnosis
with
similar
symptoms.
This
review
presents
recent
advances
used
for
diseases,
including
immunosensors
nucleic
acid
sensors.
As
representative
platforms,
lateral
flow
immunoassay
(LFIA)
polymer-based
chips,
paper-based
devices,
droplet-based
devices
will
discussed
detail.
In
addition,
current
challenges,
commercialization,
prospects
proposed
promote
application
detection.
Mitigating
the
spread
of
global
infectious
diseases
requires
rapid
and
accurate
diagnostic
tools.
Conventional
techniques
for
typically
require
sophisticated
equipment
are
time
consuming.
Emerging
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
proteins
(Cas)
detection
systems
have
shown
remarkable
potential
as
next-generation
tools
to
achieve
rapid,
sensitive,
specific,
field-deployable
diagnoses
diseases,
based
on
state-of-the-art
microfluidic
platforms.
Therefore,
a
review
recent
advances
in
CRISPR-based
diagnosis
is
urgently
required.
This
highlights
mechanisms
CRISPR/Cas
biosensing
cutting-edge
devices
including
paper,
digital,
integrated
wearable
Strategies
simplify
sample
pretreatment,
improve
performance,
discussed.
Current
challenges
future
perspectives
contributing
development
more
effective
also
proposed.
Expert Review of Molecular Diagnostics,
Год журнала:
2024,
Номер
24(4), С. 273 - 282
Опубликована: Март 19, 2024
Introduction
While
Aspergillus
spp.
remain
the
predominant
cause
of
invasive
mold
infections,
non-Aspergillus
molds,
such
as
Mucorales
or
Fusarium
spp.,
account
for
an
increasing
proportion
cases.
The
diagnosis
infections
(NAIMI)
is
challenging
because
low
sensitivity
and
delay
conventional
microbiological
tests.
Therefore,
there
a
particular
interest
to
develop
molecular
tools
their
early
detection
in
blood
other
clinical
samples.
Analytical Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Reviews
the
development
of
visual
detection
methods
in
recent
years
based
on
different
Cas
and
analyzes
their
advantages
disadvantages
as
well
challenges
future
research.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(34), С. 44485 - 44492
Опубликована: Авг. 16, 2024
This
work
proposed
a
simple
and
ultrasensitive
nanozyme-based
immunoassay
on
filtration
device
for
the
detection
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
nucleocapsid
protein
(NP).
Gold
core
porous
platinum
shell
nanoparticles
(Au@Pt
NPs)
were
synthesized
with
high
catalytic
activity
to
oxidize
3,3',5,5'-tetramethylbenzidine,
leading
an
oblivious
color
change.
The
was
designed
based
size
difference
magnetic
beads,
filter
membrane
pore,
Au@Pt
NPs.
A
simple,
rapid,
consistent
washing
procedure
can
be
performed
help
plastic
syringe.
method
could
realize
quantitative
SARS-CoV-2
NP
within
80
min
point-of-care
needs.
limit
antigen
0.01
ng/mL
in
buffer.
coefficients
variation
assay
1.78%
10
antigen,
2.03%
1
2.34%
negative
sample,
respectively.
specificity
platform
verified
by
various
viruses.
effective
system
expected
promote
substantial
progress
development
application
virus
immunodetection
technology.
