Next-generation pathogen detection: Exploring the power of nucleic acid amplification-free biosensors

Coordination Chemistry Reviews, Год журнала: 2024, Номер 513, С. 215895 - 215895

Опубликована: Май 4, 2024

Язык: Английский

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Advances in surface-enhanced Raman scattering detection of foodborne pathogens: From recognition-based fingerprint to molecular diagnosis

Coordination Chemistry Reviews, Год журнала: 2024, Номер 518, С. 216083 - 216083

Опубликована: Июль 23, 2024

Язык: Английский

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Ultrasensitive detection platform for Staphylococcus aureus based on DNAzyme tandem blocking CRISPR/Cas12a system

Biosensors and Bioelectronics, Год журнала: 2024, Номер 264, С. 116671 - 116671

Опубликована: Авг. 17, 2024

Язык: Английский

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One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives

Trends in biotechnology, Год журнала: 2024, Номер 42(11), С. 1410 - 1426

Опубликована: Июль 20, 2024

Язык: Английский

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Detection of Tetracycline with a CRISPR/Cas12a Aptasensor Using a Highly Efficient Fluorescent Polystyrene Microsphere Reporter System

ACS Synthetic Biology, Год журнала: 2024, Номер 13(7), С. 2166 - 2176

Опубликована: Июнь 12, 2024

CRISPR-based diagnostics use the CRISPR-Cas system

Язык: Английский

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SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 417, С. 136196 - 136196

Опубликована: Июнь 25, 2024

Язык: Английский

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Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives

ACS Sensors, Год журнала: 2024, Номер 9(11), С. 5665 - 5682

Опубликована: Ноя. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Язык: Английский

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Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

ACS Nano, Год журнала: 2024, Номер 18(49), С. 33505 - 33519

Опубликована: Дек. 2, 2024

The development of novel detection technology for meat species authenticity is imperative. Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet platform named CC-drop (CRISPR/Cas12a digital single-molecule microdroplet biosensor). This strategy allowed us to quickly identify and analyze animal-derived components in foods. biosensor was enabled by CRISPR/Cas12a-based fluorescence lighting-up detection, the nucleic acid signals can be recognized Cas12a–crRNA binary complex trigger trans-cleavage any by-stander reporter single-stranded (ss) DNA, which converted amplified fluorescent readouts. ultralocalized reactor constructed reducing reaction volume from up picoliter accommodate aforementioned further enhance sensitivity even render an amplification-free detection. Moreover, established smartphone App coupled with random forest learning model based on parameters such as area, intensity, counting number ensure accuracy image recording processing. sample-to-result time within 80 min. Importantly, proposed able accurately detect ND1 (pork-specific) IL-2 (duck-specific) genes deep processed meat-derived foods that usually had truncated results were more robust practical than conventional real-time polymerase chain after side-by-side comparison. All all, expected used rapid food other detections future.

Язык: Английский

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A Thermostable Cas12b-Powered Bioassay Coupled with Loop-Mediated Isothermal Amplification in a Customized “One-Pot” Vessel for Visual, Rapid, Sensitive, and On-Site Detection of Genetically Modified Crops

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(19), С. 11195 - 11204

Опубликована: Апрель 2, 2024

Genetically modified crops (GMCs) have been discussed due to unknown safety, and thus, it is imperative develop an effective detection technology. CRISPR/Cas deemed a burgeoning technology for nucleic acid detection. Herein, we developed novel method the first time, which combined thermostable Cas12b with loop-mediated isothermal amplification (LAMP), detect genetically (GM) soybeans in customized one-pot vessel. In our method, LAMP-specific primers were used amplify cauliflower mosaic virus 35S promoter (CaMV35S) of GM soybean samples. The corresponding amplicons activated

Язык: Английский

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CRISPR-Based Strategies for Sample-to-Answer Monkeypox Detection: Current Status and Emerging Opportunities

Nanotechnology, Год журнала: 2024, Номер 36(4), С. 042001 - 042001

Опубликована: Окт. 21, 2024

Abstract The global health threat posed by the Monkeypox virus (Mpox) requires swift, simple, and accurate detection methods for effective management, emphasizing growing necessity decentralized point-of-care (POC) diagnostic solutions. clustered regularly interspaced short palindromic repeats (CRISPR), initially known its nucleic acid abilities, presents itself as an attractive strategy. CRISPR offers exceptional sensitivity, single-base specificity, programmability. Here, we reviewed latest developments in CRISPR-based POC devices testing strategies Mpox detection. We explored crucial role of genetic sequencing designing crRNA reaction understanding transmission mutations. Additionally, showed integration CRISPR-Cas12 strategy with pre-amplification amplification-free methods. Our study also focused on significant Cas12 proteins effectiveness coupled recombinase polymerase amplification (RPA) envision future prospects challenges, positioning CRISPR-Cas12-based a frontrunner next generation molecular biosensing technologies.

Язык: Английский

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