Functional photoacoustic imaging: from nano- and micro- to macro-scale

Nano Convergence, Год журнала: 2023, Номер 10(1)

Опубликована: Июнь 19, 2023

Functional photoacoustic imaging is a promising biological technique that offers such unique benefits as scalable resolution and depth, well the ability to provide functional information. At nanoscale, has provided super-resolution images of surface light absorption characteristics materials single organelles in cells. microscopic macroscopic scales. techniques have precisely measured quantified various physiological parameters, oxygen saturation, vessel morphology, blood flow, metabolic rate oxygen, both human animal subjects. This comprehensive review provides an overview across multiple scales, from nano macro, highlights recent advances technology developments applications. Finally, surveys future prospects biomedical field.

Язык: Английский

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Multimodal optoacoustic imaging: methods and contrast materials

Chemical Society Reviews, Год журнала: 2024, Номер 53(12), С. 6068 - 6099

Опубликована: Янв. 1, 2024

Combining optoacoustics with other imaging techniques helps bring its powerful label-free functional readings and molecular sensitivity into precise anatomical context while providing an essential cross-validation against established methods.

Язык: Английский

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Tumor Vascular Destruction and cGAS‐STING Activation Induced by Single Drug‐Loaded Nano‐Micelles for Multiple Synergistic Therapies of Cancer

Small, Год журнала: 2023, Номер 19(46)

Опубликована: Июль 20, 2023

Abstract Cancer and its metastasis/recurrence still threaten human health, despite various advanced treatments being employed. It is of great significance to develop simple drug formulations enhance the efficacy synergistic integration monotherapies. Herein, DMXAA, a vasodestructive agent with cGAS‐STING stimulation capacity, integrated polyethylene glycol grafted poly (lactic‐ co ‐glycolic) acid co‐polymer (PLGA‐PEG), obtaining PLGA‐PEG/DMXAA (PPD) nanoparticles induce tumor‐specific vascular destruction for multiple therapies cancer. PPD could formation blood clots in tumor after intravenous injection, which subsequently mediate photothermal therapy further promote release oxygen enhanced radiotherapy. Meanwhile, injury can perfect starvation tumor. More importantly, PPD‐mediated trigger potent systemic anti‐tumor immunity via inducing immunogenic death cells activating pathway. Together anti‐PD‐L1, not only remove primary tumors, but also effectively eliminate distant metastasis, recurrence. Therefore, modulation composition induced by single drug‐loaded nano‐micelle be utilized therapeutic effect antitumor response, providing practical strategy cancer therapy.

Язык: Английский

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Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Nature Protocols, Год журнала: 2023, Номер 18(7), С. 2124 - 2142

Опубликована: Май 19, 2023

Язык: Английский

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Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice

Advanced Science, Год журнала: 2022, Номер 10(3)

Опубликована: Ноя. 27, 2022

Abstract Functional magnetic resonance imaging (fMRI) has massively contributed to the understanding of mammalian brain function. However, origin and interpretation blood oxygen level‐dependent (BOLD) signals retrieved by fMRI remain highly disputed. This article reports on development a fully hybridized system enabling concurrent functional optoacoustic tomography (MROT) measurements stimulus‐evoked brain‐wide sensory responses in mice. The complementary angiographic soft tissue contrasts both modalities along with simultaneous multi‐parametric readings hemodynamic are leveraged order establish unequivocal links between various counteracting physiological metabolic processes brain. results indicate that BOLD correlated, spatially temporally, total hemoglobin resolved volumetric multi‐spectral tomography. Furthermore, differential oxygenated deoxygenated exhibit superior sensitivity as compared when detecting responses. MROT approach greatly expands neuroimaging toolset comprehensively study neurovascular neurometabolic coupling mechanisms related diseases.

Язык: Английский

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Unsupervised Fusion of Misaligned PAT and MRI Images via Mutually Reinforcing Cross-Modality Image Generation and Registration

IEEE Transactions on Medical Imaging, Год журнала: 2023, Номер 43(5), С. 1702 - 1714

Опубликована: Дек. 26, 2023

Photoacoustic tomography (PAT) and magnetic resonance imaging (MRI) are two advanced techniques widely used in pre-clinical research. PAT has high optical contrast deep range but poor soft tissue contrast, whereas MRI provides excellent information temporal resolution. Despite recent advances medical image fusion with pre-aligned multimodal data, PAT-MRI remains challenging due to misaligned images spatial distortion. To address these issues, we propose an unsupervised multi-stage learning framework called PAMRFuse for fusion. comprises a unimodal registration network accurately align the input pairs self-attentive that selects information-rich features We employ end-to-end mutually reinforcing mode our network, which enables joint optimization of cross-modality generation registration. best knowledge, this is first attempt at MRI. Qualitative quantitative experimental results show performance method fusing small animals captured from commercial systems.

Язык: Английский

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Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 25, 2024

Abstract Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding function. The existing neuroimaging techniques are largely limited by restricted field view, slow speed, or otherwise do not have the adequate spatial to capture activities on a capillary and cellular level. To address these limitations, we introduce fluorescence localization microscopy aided sparsely-labeled red blood cells cortex-wide morphological functional cerebral angiography 4.9 µm 1 s temporal resolution. When combined calcium imaging, proposed method enables extended recordings stimulus-evoked neuro-vascular changes in murine while providing simultaneous multiparametric readings intracellular neuronal activity, flow velocity/direction/volume, vessel diameter. Owing its simplicity versatility, approach will become an invaluable tool deciphering regulation cortical microcirculation neurovascular coupling health disease.

Язык: Английский

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Multimodal imaging of murine cerebrovascular dynamics induced by transcranial pulse stimulation

Alzheimer s & Dementia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Transcranial pulse stimulation (TPS) is increasingly being investigated as a promising potential treatment for Alzheimer's disease (AD). Although the safety and preliminary clinical efficacy of TPS short pulses have been supported by neuropsychological scores in treated AD patients, its fundamental mechanisms are uncharted. Herein, we used multi-modal preclinical imaging platform combining real-time volumetric optoacoustic tomography, contrast-enhanced magnetic resonance imaging, ex vivo immunofluorescence to comprehensively analyze structural hemodynamic effects induced TPS. Cohorts healthy transgenic mice were imaged during after exposure at various per-pulse energy levels. enhanced microvascular network, whereas blood-brain barrier remained intact following procedure. Notably, higher energies necessary induce changes mice, arguably due their impacted vessels. These findings shed light on cerebrovascular dynamics treatment, hence expected assist improving therapeutic outcomes. ·Transcranial facilitates transcranial wave propagation using avoid tissue heating. ·Preclinical combines (OA) (CE-MRI), ·Blood volume enhancement networks was reproducibly observed with OA stimulation. ·CE-MRI gross pathology further confirmed that brain architecture maintained without (BBB) opening exposure, thus validating ·Higher compared wild-type animals, pathological

Язык: Английский

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Elucidating hemodynamics and neuro-glio-vascular signaling using rodent fMRI

Trends in Neurosciences, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Despite extensive functional mapping studies using rodent magnetic resonance imaging (fMRI), interpreting the fMRI signals in relation to their neuronal origins remains challenging due hemodynamic nature of response. Ultra high-resolution fMRI, beyond merely enhancing spatial specificity, has revealed vessel-specific responses, highlighting distinct contributions intracortical arterioles and venules signals. This 'single-vessel' approach shifts paradigm enabling its integration with other neuroimaging modalities investigate neuro-glio-vascular (NGV) signaling underlying a variety brain dynamics. Here, we review emerging trend combining multimodal opto/chemogenetic neuromodulation genetically encoded biosensors for cellular circuit-specific recording, offering unprecedented opportunities cross-scale dynamic models.

Язык: Английский

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Multiparametric super-resolution optoacoustic imaging with ICG-tagged microbubbles

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101865 - 101865

Опубликована: Май 1, 2025

Язык: Английский

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