Promising dawn in tumor microenvironment therapy: engineering oral bacteria

International Journal of Oral Science, Год журнала: 2024, Номер 16(1)

Опубликована: Март 13, 2024

Abstract Despite decades of research, cancer continues to be a major global health concern. The human mouth appears multiplicity local environments communicating with other organs and causing diseases via microbes. Nowadays, the role oral microbes in development progression has received increasing scrutiny. At same time, bioengineering technology nanotechnology is growing rapidly, which physiological activities natural bacteria are modified improve therapeutic efficiency cancers. These engineered were transformed achieve directed genetic reprogramming, selective functional reorganization precise control. In contrast endotoxins produced by typical genetically bacteria, flora exhibits favorable biosafety characteristics. To outline current cognitions upon microbes, cancers, related literatures searched reviewed based on PubMed database. We focused number mechanisms associated tumor microenvironment, involve occurrence development. Whether engineering can possible application therapy worth consideration. A deeper understanding relationship between may enhance our knowledge pathogenesis thus providing new insights strategies for prevention treatment.

Язык: Английский

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Engineered bacterial outer membrane vesicles: a versatile bacteria-based weapon against gastrointestinal tumors

Theranostics, Год журнала: 2024, Номер 14(2), С. 761 - 787

Опубликована: Янв. 1, 2024

Outer membrane vesicles (OMVs) are nanoscale lipid bilayer structures released by gram-negative bacteria.They share composition and properties with their originating cells, making them adept at traversing cellular barriers.These OMVs have demonstrated exceptional stability, immunogenicity, safety, penetration, tumor-targeting properties, which been leveraged in developing vaccines drug delivery systems.Recent research efforts focused on engineering to increase production yield, reduce cytotoxicity, improve the safety efficacy of treatment.Notably, gastrointestinal (GI) tumors proven resistant several traditional oncological treatment strategies, including chemotherapy, radiotherapy, targeted therapy.Although immune checkpoint inhibitors some patients, usage as monotherapy remains limited tumor heterogeneity individual variability.The immunogenic modifiable nature makes an ideal design platform for individualized GI tumors.OMV-based therapy enables combination optimization anti-tumor effects.This review comprehensively summarizes recent advances OMV discusses challenges clinical translation emerging OMV-based therapies.

Язык: Английский

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Bacterial‐Mediated Tumor Therapy: Old Treatment in a New Context

Advanced Science, Год журнала: 2023, Номер 10(12)

Опубликована: Март 12, 2023

Abstract Targeted therapy and immunotherapy have brought hopes for precision cancer treatment. However, complex physiological barriers tumor immunosuppression result in poor efficacy, side effects, resistance to antitumor therapies. Bacteria‐mediated provides new options address these challenges. Thanks their special characteristics, bacteria excellent ability destroy cells from the inside induce innate adaptive immune responses. Furthermore, bacterial components, including vesicles, spores, toxins, metabolites, other active substances, similarly inherit unique targeting properties capabilities. Bacteria accessory products can even be reprogrammed produce deliver agents according clinical needs. This review first discusses role of different development tumorigenesis latest advances bacteria‐based delivery platforms existing obstacles application. Moreover, prospect challenges transformation engineered are also summarized.

Язык: Английский

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Therapeutic bacteria and viruses to combat cancer: double-edged sword in cancer therapy: new insights for future

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Апрель 24, 2024

Abstract Cancer, ranked as the second leading cause of mortality worldwide, leads to death approximately seven million people annually, establishing itself one most significant health challenges globally. The discovery and identification new anti-cancer drugs that kill or inactivate cancer cells without harming normal healthy reduce adverse effects on immune system is a potential challenge in medicine fundamental goal Many studies. Therapeutic bacteria viruses have become dual-faceted instrument therapy. They provide promising avenue for treatment, but at same time, they also create obstacles complications contribute growth development. This review article explores role examining their benefits drawbacks. By amalgamating established knowledge perspectives, this offers an in-depth examination present research landscape within domain identifies avenues future investigation. Graphical double-edged sword

Язык: Английский

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Living Bacteria: A New Vehicle for Vaccine Delivery in Cancer Immunotherapy

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2056 - 2056

Опубликована: Фев. 26, 2025

Cancer vaccines, aimed at evolving the human immune system to eliminate tumor cells, have long been explored as a method of cancer treatment with significant clinical potential. Traditional delivery systems face challenges in directly targeting cells and delivering adequate amounts antigen due hostile microenvironment. Emerging evidence suggests that certain bacteria naturally home on tumors modulate antitumor immunity, making bacterial vectors promising vehicle for precision vaccines. Live vehicles offer several advantages, including colonization, precise drug delivery, stimulation, them compelling option immunotherapy. In this review, we explore mechanisms action behind living bacteria-based recent progress popular chassis, strategies specific payload biocontainment ensure safety. These approaches will lay foundation developing an affordable, widely applicable vaccine system. This review also discusses future opportunities harnessing bacterial-based vaccines enhanced therapeutic outcomes treatment.

Язык: Английский

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Neutrophil hitchhiking nanoparticles enhance bacteria-mediated cancer therapy via NETosis reprogramming

Journal of Controlled Release, Год журнала: 2024, Номер 367, С. 661 - 675

Опубликована: Фев. 8, 2024

Язык: Английский

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Engineered Microorganisms for Advancing Tumor Therapy

Advanced Materials, Год журнала: 2024, Номер 36(24)

Опубликована: Март 15, 2024

Engineered microorganisms have attracted significant interest as a unique therapeutic platform in tumor treatment. Compared with conventional cancer treatment strategies, engineering microorganism-based systems provide various distinct advantages, such the intrinsic capability targeting tumors, their inherent immunogenicity, situ production of antitumor agents, and multiple synergistic functions to fight against tumors. Herein, design, preparation, application engineered for advanced therapy are thoroughly reviewed. This review presents comprehensive survey innovative strategies based on series representative microorganisms, including bacteria, viruses, microalgae, fungi. Specifically, it offers extensive analyses design principles, mechanisms, well advantages limitations different systems. Finally, current challenges future research prospects this field, which can inspire new ideas creative paradigms utilizing facilitate clinical applications, discussed.

Язык: Английский

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Microbe-material hybrids for therapeutic applications

Chemical Society Reviews, Год журнала: 2024, Номер 53(16), С. 8306 - 8378

Опубликована: Янв. 1, 2024

As natural living substances, microorganisms have emerged as useful resources in medicine for creating microbe-material hybrids ranging from nano to macro dimensions. The engineering of microbe-involved nanomedicine capitalizes on the distinctive physiological attributes microbes, particularly their intrinsic "living" properties such hypoxia tendency and oxygen production capabilities. Exploiting these remarkable characteristics combination with other functional materials or molecules enables synergistic enhancements that hold tremendous promise improved drug delivery, site-specific therapy, enhanced monitoring treatment outcomes, presenting substantial opportunities amplifying efficacy disease treatments. This comprehensive review outlines microbial derivatives used biomedicine specific advantages therapeutic application. In addition, we delineate fundamental strategies mechanisms employed constructing hybrids. diverse biomedical applications constructed hybrids, encompassing bioimaging, anti-tumor, anti-bacteria, anti-inflammation diseases therapy are exhaustively illustrated. We also discuss current challenges prospects associated clinical translation hybrid platforms. Therefore, unique versatility potential exhibited by position them promising candidates development next-generation biomaterials theranostic functionalities.

Язык: Английский

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Studying the Oncolytic Activity of Streptococcus pyogenes Strains Against Hepatoma, Glioma, and Pancreatic Cancer In Vitro and In Vivo

Microorganisms, Год журнала: 2025, Номер 13(1), С. 76 - 76

Опубликована: Янв. 3, 2025

Background: Cancer remains a leading cause of mortality globally. Conventional treatment modalities, including radiation and chemotherapy, often fall short achieving complete remission, highlighting the critical need for novel therapeutic strategies. One promising approach involves oncolytic potential Group A Streptococcus (GAS) strains tumor treatment. This study aimed to investigate efficacy S. pyogenes GUR its M protein knockout mutant, strain GURSA1, which was genetically constructed minimize overall toxicity, against mouse hepatoma 22A, pancreatic cancer PANC02, human glioma U251 cells, both in vitro vivo, using C57BL/6 model. Methods: The cytotoxic activity GAS studied U251, murine 22a, normal skin fibroblast cells MTT assay real-time xCELLigence system. syngeneic model used evaluate vivo effect strains. Statistical analysis conducted Student’s t-test Mann–Whitney U-test with GraphPad Prism software. Results: showed that live had strong (67.4 ± 1.9%) PANC02 cells. exhibited moderate (38.0 1.8%) weak (16.3 5.4%) activities respectively. In contrast, GURSA1 demonstrated (86.5 1.6%) (36.5 Additionally, did not exhibit healthy (cell viability 104.2 1.3%, p = 0.2542). We significantly increased lifespan mice (34 days, 0.040) (32 0.039) relative control groups (24 28 respectively). Increased accompanied by slowdown progression, as evidenced reduction growth tumors under mice. Conclusions: Both vitro. show toxicity fibroblasts. survival rate treated lacking on surface, were higher compared groups.

Язык: Английский

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Bacterial-Mediated In Situ Engineering of Tumour-Associated Macrophages for Cancer Immunotherapy

Cancers, Год журнала: 2025, Номер 17(5), С. 723 - 723

Опубликована: Фев. 20, 2025

Background/Objectives: Tumour-associated macrophages (TAMs) are critical components of the tumour microenvironment (TME), significantly influencing cancer progression and treatment resistance. This review aims to explore innovative use engineered bacteria reprogram TAMs, enhancing their anti-tumour functions improving therapeutic outcomes. Methods: We conducted a systematic following predefined protocol. Multiple databases were searched identify relevant studies on phenotypic plasticity, for reprogramming. Inclusion exclusion criteria applied select studies, data extracted using standardised forms. Data synthesis was performed summarise findings, focusing mechanisms benefits non-pathogenic modify TAMs. Results: The summarises findings that can selectively target promoting shift from tumour-promoting M2 phenotype tumour-fighting M1 phenotype. reprogramming enhances pro-inflammatory responses activity within TME. Evidence various indicates significant regression improved immune bacterial therapy. Conclusions: Reprogramming TAMs presents promising strategy approach leverages natural targeting abilities directly tumour, potentially patient outcomes offering new insights into immune-based treatments. Further research is needed optimise these methods assess clinical applicability.

Язык: Английский

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