Theranostics,
Год журнала:
2023,
Номер
14(1), С. 283 - 303
Опубликована: Ноя. 15, 2023
Rationale:
Intracerebral
hemorrhage
(ICH)
is
a
devastating
cerebrovascular
disease
resulting
from
blood
extravasating
into
the
brain
parenchyma.Escalation
of
erythrophagocytosis
(a
form
efferocytosis),
avoiding
consequent
release
detrimental
erythrocyte
lysates,
may
be
promising
target
ICH
management.The
ADAM17
inhibitor
and
liver
X
receptor
(LXR)
agonist
could
promote
efficient
efferocytosis
injury
repair.Nevertheless,
poor
bioavailability
restriction
blood-brain
barrier
(BBB)
hinder
their
application.Therefore,
it
needed
that
biocompatible
smart
nanoplatforms
were
designed
synthesized
to
realize
effective
therapy
targeting
erythrophagocytosis.Methods:
We
first
assessed
synergistic
effect
therapeutic
GW280264X
(an
inhibitor)
desmosterol
LXR
agonist)
on
in
vitro.Then
pH-responsive
neutrophil
membrane-based
nanoplatform
(NPEOz)
served
as
carrier
accurately
deliver
damaged
was
prepared
via
co-extrusion.Afterwards,
performance
valued
vitro
ability
through
fluorescence
image
vivo.Finally,
pro-erythrophagocytic
anti-neuroinflammatory
nanomedicine
related
mechanisms
investigated.Results:
After
synergistical
above
two
drugs
confirmed,
we
successfully
developed
neutrophil-disguised
nanoparticles
efficiently
co-deliver
them.The
responsively
agents
under
acidic
environments,
elicit
favorable
biocompatibility
sites.D&G@NPEOz
enhanced
inhibiting
shedding
efferocytotic
receptors
MERTK/AXL
mediated
by
accelerating
ABCA-1/ABCG-1-mediated
cholesterol
efflux
regulated
respectively.In
addition,
nano-formulation
able
modulate
inflammatory
microenvironment
transforming
efferocytes
towards
phenotype
with
reducing
proinflammatory
cytokines
while
increasing
secretion
anti-inflammatory
factors,
improve
neurological
function.Conclusions:
This
biomimetic
envisaged
offer
an
encouraging
strategy
effectively
hematoma
inflammation
resolution,
consequently
alleviate
progression.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 4021 - 4040
Опубликована: Май 1, 2024
Abstract:
Cataract
is
a
leading
cause
of
blindness
globally,
and
its
surgical
treatment
poses
significant
burden
on
global
healthcare.
Pharmacologic
therapies,
including
antioxidants
protein
aggregation
reversal
agents,
have
attracted
great
attention
in
the
cataracts
recent
years.
Due
to
anatomical
physiological
barriers
eye,
effectiveness
traditional
eye
drops
for
delivering
drugs
topically
lens
hindered.
The
advancements
nanomedicine
present
novel
promising
strategies
addressing
challenges
drug
delivery
lens,
development
nanoparticle
formulations
that
can
improve
penetration
into
anterior
segment
enable
sustained
release
medications.
This
review
introduces
various
cutting-edge
systems
cataract
treatment,
highlighting
their
physicochemical
properties
surface
engineering
optimal
design,
thus
providing
impetus
further
innovative
research
potential
clinical
applications
anti-cataract
drugs.
Keywords:
nanomedicine,
cataract,
ocular
barrier,
system
Neural Regeneration Research,
Год журнала:
2024,
Номер
20(6), С. 1665 - 1680
Опубликована: Май 10, 2024
Spinal
cord
injuries
lead
to
significant
loss
of
motor,
sensory,
and
autonomic
functions,
presenting
major
challenges
in
neural
regeneration.
Achieving
effective
therapeutic
concentrations
at
injury
sites
has
been
a
slow
process,
partly
due
the
difficulty
delivering
drugs
effectively.
Nanoparticles,
with
their
targeted
delivery
capabilities,
biocompatibility,
enhanced
bioavailability
over
conventional
drugs,
are
garnering
attention
for
spinal
treatment.
This
review
explores
current
mechanisms
shortcomings
existing
treatments,
highlighting
benefits
progress
nanoparticle-based
approaches.
We
detail
nanoparticle
methods
injury,
including
local
intravenous
injections,
oral
delivery,
biomaterial-assisted
implantation,
alongside
strategies
such
as
drug
loading
surface
modification.
The
discussion
extends
how
nanoparticles
aid
reducing
oxidative
stress,
dampening
inflammation,
fostering
regeneration,
promoting
angiogenesis.
summarize
use
various
types
treating
injuries,
metallic,
polymeric,
protein-based,
inorganic
non-metallic,
lipid
nanoparticles.
also
discuss
faced,
biosafety,
effectiveness
humans,
precise
dosage
control,
standardization
production
characterization,
immune
responses,
vivo.
Additionally,
we
explore
future
directions,
improving
standardizing
manufacturing
characterization
processes,
advancing
human
trials.
Nanoparticles
have
shown
considerable
enhancing
treatment
efficacy
potential
clinical
development.
Macrophages
are
rapidly
activated
and
polarized
toward
the
M1
phenotype
after
spinal
cord
injury
(SCI),
inhibiting
M1-like
macrophages
has
emerged
as
a
promising
SCI
treatment
approach.
Metalloregulatory
proteins,
which
sense
specific
metal
ions
with
high
affinity
specificity,
play
critical
role
in
immune
regulation.
Here,
we
screened
various
bioactive
associated
metalloregulatory
proteins
discovered
that
Zn2+
Mn2+
effectively
suppressed
polarization.
Based
on
these
findings,
mildly
alkaline
ZnMn-based
layered
double
hydroxides
(ZnMn-LDHs)
self-assembled
from
coordinated
were
developed
to
inhibit
macrophages.
ZnMn-LDHs
neutralized
acidic
environment
promoted
expression
of
including
metallothionein
(MT),
superoxide
dismutase
1
(SOD1),
2
(SOD2),
thereby
eliciting
robust
macrophage
inhibition.
More
importantly,
nerve
growth
factor
(NGF)
released
by
following
regulation
elongation
spreading
Schwann
cells.
By
integrating
silk
fibroin
(SF),
ZnMn@SF
injectable
hydrogels
constructed
for
repair.
An
vivo
animal
model
further
revealed
excellent
anti-inflammatory
effects
treating
SCI,
functional
recovery.
Our
findings
underscore
importance
regulated
macrophages,
providing
therapeutic
strategy
treatment.
Abstract
Musculoskeletal
(MSK)
disorders
significantly
burden
patients
and
society,
resulting
in
high
healthcare
costs
productivity
loss.
These
are
the
leading
cause
of
physical
disability,
their
prevalence
is
expected
to
increase
as
sedentary
lifestyles
become
common
global
population
elderly
increases.
Proper
innervation
critical
maintaining
MSK
function,
nerve
damage
or
dysfunction
underlies
various
disorders,
underscoring
potential
restoring
function
disorder
treatment.
However,
most
tissue
engineering
strategies
have
overlooked
significance
innervation.
This
review
first
expounds
upon
system
its
importance
homeostasis
functions.
will
be
followed
by
for
tissues
that
induce
post‐implantation
situ
pre‐innervated.
Subsequently,
research
progress
modeling
using
innervated
organoids
organs‐on‐chips
(OoCs)
analyzed.
Finally,
future
development
treat
recapitulate
disease
mechanisms
discussed.
provides
valuable
insights
into
underlying
principles,
methods,
applications
tissues,
paving
way
targeted,
efficacious
therapies
conditions.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
Abstract
Spinal
cord
injury
(SCI)
impairs
the
central
nervous
system
and
induces
myelin‐sheath‐deterioration
because
of
reactive
oxygen
species
(ROS),
further
hindering
recovery
function.
Herein,
simultaneously
emergency
treatment
dynamic
luminescence
severity
assessment
(SETLSA)
strategy
is
designed
for
SCI
based
on
cerium
(Ce)‐doped
upconversion
antioxidant
nanoenzymes
(Ce@UCNP‐BCH).
Ce@UCNP‐BCH
can
not
only
efficiently
eliminate
localized
ROS,
but
dynamically
monitor
oxidative
state
in
repair
process
using
a
ratiometric
signal.
Moreover,
classic
basso
mouse
scale
score
immunofluorescence
analysis
together
exhibit
that
effectively
facilitates
regeneration
spinal
including
myelin
sheath,
promotes
functional
mice.
Particularly,
study
combines
snATAC‐eq
snRNA‐seq
to
reveal
heterogeneity
tissue
following
treatment.
The
findings
significant
increase
myelinating
oligodendrocytes,
as
well
higher
expression
myelination‐related
genes,
also
reveals
gene
regulatory
dynamics
remyelination
after
Besides,
ETLSA
synergistically
boosts
ROS
consumption
through
superoxide
dismutase
(SOD)‐related
pathways
SOD‐siRNA
In
conclusion,
this
SETLSA
with
blocking
monitoring
stress
has
enriched
toolkit
promoting
repair.
Abstract
The
establishment
of
new
functional
connections
through
axon
regeneration
is
essential
for
recovery
following
spinal
cord
injury
(SCI).
With
the
rapid
advancement
nanotechnology,
nanomaterial
(NM)‐based
therapies
have
emerged
as
promising
approaches
to
promote
after
SCI.
Specifically,
NMs
can
limit
spread
damage,
maintain
homeostasis
in
extracellular
environment
surrounding
injured
axons,
and
ultimately
facilitate
growth
In
particular,
with
enzyme‐like
properties,
such
cerium
oxide
manganese
dioxide,
clear
reactive
oxygen
species
damaged
area,
thereby
promoting
regeneration.
Additionally,
electromagnetic
properties
guide
under
influence
magnetic
or
electric
fields.
Importantly,
biological
mechanisms
by
which
are
summarized
discussed
explore
NM‐based
strategies
achieving
Together,
elucidating
physicochemical
basis
their
promotion
SCI
help
fill
gaps
our
understanding
how
be
used
treat
further
clinical
translation
future.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Сен. 16, 2024
Nanozymes,
a
category
of
nanomaterials
with
enzyme-like
activity,
have
garnered
growing
interest
in
various
biomedical
contexts.
Notably,
nanozymes
that
are
capable
regulating
reactive
oxygen
species
levels
by
emulating
antioxidant
or
prooxidant
enzymes
within
cells
hold
significant
therapeutic
potential
for
range
disorders.
Herein,
we
overview
the
catalytic
mechanisms
four
exemplary
orthopedic
domain.
Subsequently,
emphasize
recent
groundbreaking
advancements
nanozyme
applications
orthopaedics,
encompassing
osteoarthritis,
osteoporosis,
intervertebral
disc
degeneration,
bone
defects,
spinal
cord
injury,
gout,
rheumatoid
arthritis,
osteosarcoma
and
infection.
Furthermore,
discuss
emerging
area's
future
prospects
several
noteworthy
challenges
application.
This
review
not
only
fosters
ongoing
development
research
but
also
emergence
more
potent
treatment
orthopaedical
diseases
future.
