Heliyon, Год журнала: 2024, Номер 10(20), С. e39251 - e39251
Опубликована: Окт. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2023, Номер 10(20)
Опубликована: Май 10, 2023
Abstract Over the last decade, adipose‐derived stem cells (ADSCs) have attracted increasing attention in field of regenerative medicine. ADSCs appear to be most advantageous cell type for therapies owing their easy accessibility, multipotency, and active paracrine activity. This review highlights current challenges translating ADSC‐based into clinical settings discusses novel strategies overcome limitations ADSCs. To further establish as an emerging platform medicine, this also provides update on advancements field, including fat grafting, wound healing, bone regeneration, skeletal muscle repair, tendon reconstruction, cartilage cardiac nerve regeneration. are expected more tissue‐specific increasingly important
Язык: Английский
Процитировано
111
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Март 27, 2024
Abstract Musculoskeletal disorders are the leading causes of physical disabilities worldwide. The poor self-repair capacity musculoskeletal tissues and absence effective therapies have driven development novel bioengineering-based therapeutic approaches. Adipose-derived stem cell (ADSC)-based being explored as new regenerative strategies for repair regeneration bone, cartilage, tendon owing to accessibility, multipotency, active paracrine activity ADSCs. In this review, recent advances in ADSCs their optimization strategies, including ADSC-derived exosomes (ADSC-Exos), biomaterials, genetic modifications, summarized. Furthermore, preclinical clinical applications ADSC-Exos, either alone or combination with growth factors biomaterials genetically modified forms, reviewed. ADSC-based hold promise management multiple types injuries. timely summary highlights provided here could offer guidance further investigations accelerate application regeneration. Graphical abstract
Язык: Английский
Процитировано
18
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2356 - 2356
Опубликована: Фев. 16, 2024
Adipose tissue (AT) is a large and important energy storage organ as well an endocrine with critical role in many processes. Additionally, AT enormous easily accessible source of multipotent cell types used our day for all regeneration. The ability adipose-derived stem cells (ADSCs) to differentiate into other cells, such endothelial (ECs), vascular smooth muscle or cardiomyocytes, engineering order promote/stimulate the process angiogenesis. Being key future successful clinical applications, functional networks engineered are targeted by numerous vivo ex studies. article reviews angiogenic potential ADSCs explores their capacity field (TE).
Язык: Английский
Процитировано
17
Progress in Retinal and Eye Research, Год журнала: 2024, Номер 100, С. 101261 - 101261
Опубликована: Март 26, 2024
Glaucoma is the leading cause of irreversible blindness globally. The disease causes vision loss due to neurodegeneration retinal ganglion cell (RGC) projection brain through optic nerve. associated with sensitivity intraocular pressure (IOP). Thus, mainstay treatments seek manage IOP, though many patients continue lose vision. To address directly, numerous preclinical studies develop protective or reparative therapies that act independently IOP. These include growth factors, compounds targeting metabolism, anti-inflammatory and antioxidant agents, neuromodulators. Despite success in experimental models, these approaches fail translate into clinical benefits. Several factors contribute this challenge. Firstly, anatomic structure nerve head differs between rodents, nonhuman primates, humans. Additionally, animal models do not replicate complex glaucoma pathophysiology Therefore, enhance translating findings, we propose two approaches. First, thorough evaluation targets multiple including should precede trials. Second, advocate for combination therapy, which involves using agents simultaneously, especially early potentially reversible stages disease. strategies aim increase chances successful neuroprotective treatment glaucoma.
Язык: Английский
Процитировано
16
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Март 15, 2024
Abstract The challenges posed by delayed atrophic healing and nonunion stand as formidable obstacles in osteoporotic fracture treatment. processes of type H angiogenesis osteogenesis emerge pivotal mechanisms during bone regeneration. Notably, the preconditioning adipose-derived stem cell (ADSC) exosomes under hypoxic conditions has garnered attention for its potential to augment secretion functionality these exosomes. In present investigation, we embarked upon a comprehensive elucidation underlying hypo-ADSC-Exos within milieu Our findings revealed that harboured preeminent miRNA, namely, miR-21-5p, which emerged principal orchestrator angiogenic effects. Through vitro experiments, demonstrated capacity stimulate proliferation, migration, human umbilical vein endothelial cells (HUVECs) via mediation miR-21-5p. inhibition miR-21-5p effectively attenuated proangiogenic effects mediated hypo-ADSC-Exos. Mechanistically, our investigation exosomal emanating from hypo-ADSCs exerts regulatory influence targeting sprouly1 (SPRY1) HUVECs, thereby facilitating activation PI3K/AKT signalling pathway. knockdown SPRY1 HUVECs was found potentiate and, concomitantly, HUVEC angiogenesis. culminating stage study involved compelling vivo demonstration wherein GelMA loaded with validated substantially enhance local concomitant This enhancement unequivocally attributed modulation SPRY1. summary, offers pioneering perspective on utility readily available Graphical
Язык: Английский
Процитировано
15
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Июль 18, 2024
Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disease marked by synovitis and cartilage destruction. The active compound, icariin (ICA), derived from the herb Epimedium, exhibits potent anti-inflammatory properties. However, its clinical utility limited water insolubility, poor permeability, low bioavailability. To address these challenges, we developed multifunctional drug delivery system—adipose-derived stem cells-exosomes (ADSCs-EXO)-ICA to target macrophages in synovial tissue modulate macrophage polarization M1 M2. High-performance liquid chromatography analysis confirmed 92.4 ± 0.008% loading efficiency for ADSCs-EXO-ICA. In vitro studies utilizing cellular immunofluorescence (IF) flow cytometry demonstrated significant inhibition of proliferation Enzyme-linked immunosorbent assay, transcriptomics, real-time quantitative PCR indicated that ADSCs-EXO-ICA promotes an M1-to-M2 phenotypic transition reducing glycolysis through ERK/HIF-1α/GLUT1 pathway. vivo, effectively accumulated joints. Pharmacodynamic assessments revealed decreased cytokine levels mitigated symptoms collagen-induced (CIA) rats. Histological micro computed tomography markedly ameliorated preserved cartilage. Further vivo suppresses promoting switch suppressing glycolysis. Western blotting supported therapeutic efficacy RA, confirming role modulating function energy metabolism regulation. Thus, this study not only introduces system significantly enhances anti-RA but also elucidates mechanism action inhibition. Graphical abstract
Язык: Английский
Процитировано
13
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Май 3, 2024
Abstract Background Adipose-derived stem cells (ASCs) represent the most advantageous choice for soft tissue regeneration. Studies proved recruitment of ASCs post injury was mediated by chemokine CXCL12, but mechanism which CXCL12 is generated after remains unclear. Migrasomes are newly discovered membrane-bound organelles that could deliver spatially and temporally in vivo. In this study, we sought to investigate whether migrasomes participate ASC-mediated Methods Discrepant asymmetrical regeneration mice model were established, HE staining, immunofluorescent western blot qPCR conducted confirm role Characterization ASC-derived carried out confocal microscopy, scanning electron transmission microscopy as well analysis. The function further testified assisting with isolated vivo vitro transwell combined co-culture system. Results Here, show first time generate enriched mediate from promote migration activating CXCR4/RhoA signaling vitro. Chemoattracted facilitate regeneration, demonstrated upregulation an adipogenesis-associated protein. This positive feed-back-loop creates a favorable microenvironment Thus, new therapeutic target Conclusions Our findings reveal previously unknown mediating generating migrasomes. can restore recruiting cells, highlighting potential application regenerative medicine.
Язык: Английский
Процитировано
11
Free Radical Biology and Medicine, Год журнала: 2025, Номер 228, С. 281 - 298
Опубликована: Янв. 5, 2025
Язык: Английский
Процитировано
2
Breast Cancer, Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
Abstract Background Adipose-derived stem cells (ADSCs)-assisted fat grafting has emerged as a widely used procedure for breast reconstruction post mastectomy and aesthetic augmentation. Given the limited cases of cancer following grafting, oncological safety this remains controversial. Methods The effects ADSCs on oncogenic features premalignant MCF-10AT were investigated using co-culture xenograft models. We further evaluated malignancy-promoting effect in 7,12-Dimethylbenz(a)anthracene (DMBA)-induced model. RNA-sequencing was performed ADSCs, cells, ADSC-co-cultured cells. Protein changes ADSC/MCF-10AT medium determined by proteomic analysis. Pathway inhibitors to investigate signaling pathways involved ADSC-induced Results found that promoted proliferation migration co-injection increased tumor incidence from 29% 58% nude mice. Additionally, grafted significantly enhanced incidence, growth, distant metastasis DMBA-induced rats, while it could not induce tumorigenesis normal tissues. Combined analysis demonstrated paracrine factors secreted robustly activated PI3K–AKT also revealed auto-activated TGF-beta Wnt co-cultured which may be synergistic formation progression. As expected, blocking these pathways, especially pathway, strongly diminished promoting suggesting their potential therapeutic targets ADSC grafting-associated tumors. Conclusions Our data illustrated between carcinogenesis through activation pathway. Based findings, we recommend pre-operative examinations risk before ADSC-associated transplantation.
Язык: Английский
Процитировано
1
Inflammation and Regeneration, Год журнала: 2023, Номер 43(1)
Опубликована: Ноя. 27, 2023
Abstract The regenerative ability of skeletal muscle (SM) in response to damage, injury, or disease is a highly intricate process that involves the coordinated activities multiple cell types and biomolecular factors. Of these, extracellular matrix (ECM) considered fundamental component SM ability. This review briefly discusses myogenesis regeneration, roles played by satellite cells (MSCs), other cells, ECM components, effects their dysregulations on these processes. In addition, we various scaffolds biomaterials used for applications, recent advances scaffold research, impacts tissue engineering especially context severe which frequently results substantial loss impaired capacity. was undertaken provide comprehensive overview stem significance enhance understanding essential role during regeneration.
Язык: Английский
Процитировано
20