Heliyon, Год журнала: 2024, Номер 10(20), С. e39251 - e39251
Опубликована: Окт. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(20)
Опубликована: Март 13, 2024
Abstract The dynamic balance between hypoxia and oxidative stress constitutes the oxygen‐related microenvironment in injured tissues. Due to variability, oxygen homeostasis is usually not a therapeutic target for It found that when administered intravenously, mesenchymal stem cells (MSCs) vitro induced apoptotic vesicles (ApoVs) exhibit similar markers wound where co‐existed, but MSCs exhibited better effects promoting angiogenesis healing. derivation pathway of ApoVs by inducing or simulate tissues improved. Two types environmental stressed are identified directly endothelial (ECs) accurate regulation vascularization. Compared normoxic hypoxic ones, oxidatively (Oxi‐ApoVs) showed strongest tube formation capacity. Different oxygen‐stressed deliver miRNAs, which leads broad upregulation EC phosphokinase activity. Finally, local delivery Oxi‐ApoVs‐loaded hydrogel microspheres promotes Oxi‐ApoV‐loaded achieve controlled ApoV release, targeting ECs reducing consumption inflammatory adapting proliferative phase Thus, biogenerated responding can promote
Язык: Английский
Процитировано
9
Nano-Micro Letters, Год журнала: 2024, Номер 17(1)
Опубликована: Сен. 29, 2024
Abstract Hydrogel scaffolds have numerous potential applications in the tissue engineering field. However, tough hydrogel implanted vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical properties. Inspired by Chinese ramen, we propose a universal fabricating method (printing-P, training-T, cross-linking-C, PTC & PCT) for fill this gap. First, 3D printing fabricates scaffold with desired structures (P). Then, could extraordinarily properties functional surface structure cycle training salting-out assistance (T). Finally, results fixed photo-cross-linking processing (C). The gelatin exhibit excellent tensile strength of 6.66 MPa (622-fold untreated) biocompatibility. Furthermore, possesses from nanometer micron millimeter, which can efficiently induce directional cell growth. Interestingly, strategy produce bionic human 10 kPa-10 changing type salt, many hydrogels, such as silk, be improved or PCT strategies. Animal experiments show that effectively promote new generation muscle fibers, blood vessels, nerves within 4 weeks, prompting rapid regeneration large-volume loss injuries.
Язык: Английский
Процитировано
9
Biosensors and Bioelectronics X, Год журнала: 2024, Номер 18, С. 100480 - 100480
Опубликована: Апрель 25, 2024
Organ-on-a-Chip, or OOC, is a widely discussed topic in science due to its many unique advantages the biomedical field. Nevertheless, there still much learn about OOC's various aspects of conception and significance for advancement medical technology future. A platform organs-on-a-chip must go through fabrication process. Various manufacturing processes were also used depending on required disease modelling drug screening. Organs chip included Brain-On-Chip, Kidney-On-Chip, Liver-On-Chip, Heart-On-Chip. In order provide new beginnings with thorough understanding we have studied most recent developments organ-on-a-chip expertise critically assessed relevant features this research.
Язык: Английский
Процитировано
8
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Июнь 1, 2024
Abstract Background Nerve guide conduits are a promising strategy for reconstructing peripheral nerve defects. Improving the survival rate of seed cells in is still challenge and microcarriers an excellent three-dimensional (3D) culture scaffold. Here, we investigate effect 3D on biological characteristics adipose mesenchymal stem (ADSCs) to evaluate efficacy chitosan filled with loaded ADSCs repairing Methods In vitro, prepared porous microspheres by modified emulsion cross-linking method loading evaluated growth status function ADSCs. vivo, ADSCs-loaded were injected into repair 12 mm sciatic defect rats. Results Compared conventional two-dimensional (2D) culture, more conducive proliferation, migration, secretion trophic factors addition, gait analysis, neuro-electrophysiology, histological evaluation nerves muscles showed that ADSC microcarrier-loaded effective improving regeneration. Conclusions The microcarrier this study has high cell engraftment good potential repair. Graphical
Язык: Английский
Процитировано
8
Biomaterials Science, Год журнала: 2024, Номер 12(10), С. 2504 - 2520
Опубликована: Янв. 1, 2024
In recent years, the design and synthesis of Janus hydrogels have witnessed a thriving development, overcoming limitations single-performance materials expanding their potential applications in tissue engineering regenerative medicine. hydrogels, with exceptional mechanical properties excellent biocompatibility, emerged as promising candidates for various biomedical applications, including therapies. this review, we present latest progress using commonly employed preparation methods. We elucidate surface interface interactions these discuss enhanced bestowed by unique "Janus" structure biomaterials. Additionally, explore facilitating therapies, such drug delivery, wound healing, engineering, biosensing. Furthermore, analyze challenges future trends associated utilization applications.
Язык: Английский
Процитировано
7
Life Sciences, Год журнала: 2024, Номер 357, С. 123066 - 123066
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
7
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(18)
Опубликована: Март 20, 2024
Efforts are made to enhance the inherent potential of extracellular vesicles (EVs) by utilizing 3D culture platforms and engineered strategies for functional cargo-loading. Three distinct types adipose mesenchymal stem cells-derived EVs (ADSCs-EVs) successfully isolated consisting porous gelatin methacryloyl (PG), PG combined with sericin (PG/SerMA), or chondroitin sulfate (PG/ChSMA). These correspond PG-EVs, PG/SerMA-EVs, PG/ChSMA-EVs, respectively. Unique microRNA (miRNA) profiles observed in each type ADSCs-EVs. Notably, PG-EVs encapsulate higher levels hsa-miR-455-3p deliver more chondrocytes, which results activation hsa-miR-455-3p/PAK2/Smad2/3 axis subsequent hyaline cartilage regeneration. Furthermore, functionality is optimized through strategies, including agomir/lentivirus transfection, electroporation, Exo-Fect transfection. referred as Agomir-EVs, Lentivirus-EVs, Electroporation-EVs, Exo-Fect-EVs, respectively, ranked based on their efficacy encapsulating hsa-miR-455-3p, delivering promoting formation via axis. Exo-Fect-EVs exhibit highest efficiency. Collectively, conditions have an impact miRNA regeneration capabilities The findings provide valuable insights into mechanisms underlying promotion
Язык: Английский
Процитировано
6
Cells, Год журнала: 2024, Номер 13(16), С. 1384 - 1384
Опубликована: Авг. 20, 2024
Scars may represent more than a cosmetic concern for patients; they impose functional limitations and are frequently associated with the sensation of itching or pain, thus impacting both psychological physical well-being. From an aesthetic perspective, scars display variances in color, thickness, texture, contour, their homogeneity, while aspect encompasses considerations functionality, pliability, sensory perception. located critical anatomic areas have potential to induce profound impairments, including contracture-related mobility restrictions, thereby significantly daily functioning quality life. Conventional approaches scar management suffice certain extent, yet there cases where tailored interventions warranted. Autologous fat grafting emerges as promising therapeutic avenue such instances. Fundamental mechanisms underlying formation include chronic inflammation, fibrogenesis dysregulated wound healing, among other contributing factors. These can potentially be alleviated through application adipose-derived stem cells, which principal cellular component utilized process lipofilling. Adipose-derived cells possess capacity secrete proangiogenic factors fibroblast growth factor, vascular endothelial factor hepatocyte well neurotrophic factors, brain-derived Moreover, exhibit multipotency, remodel extracellular matrix, act paracrine manner, exert immunomodulatory effects cytokine secretion. molecular processes contribute neoangiogenesis, alleviation promotion conducive milieu healing. Beyond obvious benefit restoring volume, regenerative capacities facilitate reduction pruritus, fibrosis. This review elucidates autologous its beneficial on outcomes when applied tissue.
Язык: Английский
Процитировано
6
Current Stem Cell Research & Therapy, Год журнала: 2024, Номер 19(11), С. 1472 - 1483
Опубликована: Янв. 9, 2024
In recent times, it has been recognized that mesenchymal stem cells (MSCs) possess the capability to address osteoarthritis (OA). The objective of this research was examine impact injecting human adipose-derived (hADSCs) into a novel rabbit model with dual damage.
Язык: Английский
Процитировано
5
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Фев. 20, 2024
The incidence of lumbar disc herniation has gradually increased in recent years, and most patients have symptoms low back pain nerve compression, which brings a heavy burden to society alike. Although the causes are complex, intervertebral degeneration (IDD) is considered be common factor. (IVD) composed upper lower cartilage endplates, nucleus pulposus, annulus fibrosus. Aging, abnormal mechanical stress load, metabolic disorders can exacerbate progression IDD. Among them, high glucose high-fat diets (HFD) lead fat accumulation, metabolism, inflammation, important factors affecting homeostasis Diabetes advanced glycation end products (AGEs) accumulation- various adverse effects on IVD, including cell senescence, apoptosis, pyroptosis, proliferation, Extracellular matrix (ECM) degradation. While current research provides fundamental basis for treatment glucose-induced IDD patients. further exploration into mechanisms metabolism development targeted drugs will provide foundation effective these We aimed systematically review studies regarding hyperglycemia progress
Язык: Английский
Процитировано
5