Science Bulletin, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(13)
Опубликована: Янв. 29, 2024
Abstract Delayed wound healing is a major complication of diabetes, and associated with impaired cellular functions. Current treatments are unsatisfactory. Based on the previous reports microRNA expression in small extracellular vesicles (sEVs), miR‐17‐5p‐engineered sEVs (sEVs 17‐OE ) encapsulated them gelatin methacryloyl (GelMA) hydrogel for diabetic wounds treatment fabricated. SEVs successfully fabricated 16‐fold increase miR‐17‐5p expression. inhibited senescence promoted proliferation, migration, tube formation high glucose‐induced human umbilical vein endothelial cells (HG‐HUVECs). Additionally, also performs promotive effect dermal fibroblasts (HG‐HDFs). Mechanism analysis showed expressions p21 phosphatase tensin homolog (PTEN), as target genes miR‐17‐5p, downregulated significantly by . Accordingly, downstream pathways PTEN, activated. Next, loaded GelMA to fabricate novel bioactive dressing evaluate their effects healing. Gel‐sEVs effectively accelerated promoting angiogenesis collagen deposition. The mechanism may be local cell proliferation. Therefore, loading hydrogel, offering an option chronic management
Язык: Английский
Процитировано
26
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 21, 2025
Nucleic acid therapeutics represent a highly promising treatment approach in modern medicine, treating diseases at the genetic level. However, these face numerous challenges practical applications, particularly regarding their stability, effectiveness, cellular uptake efficiency, and limitations delivering them specifically to target tissues. To overcome obstacles, researchers have developed various innovative delivery systems, including viral vectors, lipid nanoparticles, polymer inorganic protein carriers, exosomes, antibody oligonucleotide conjugates, DNA nanostructure-based systems. These systems enhance therapeutic efficacy of nucleic drugs by improving targeting specificity, half-life vivo. In this review, we systematically discuss different types drugs, analyze major barriers encountered delivery, summarize current research progress emerging We also highlight latest advancements application for diseases, infectious cancer, brain wound healing. This review aims provide comprehensive overview drug systems' status future directions integrating nanotechnology, biomaterials science, gene editing technologies, emphasizing transformative potential precision medicine.
Язык: Английский
Процитировано
2
Theranostics, Год журнала: 2024, Номер 14(11), С. 4198 - 4217
Опубликована: Янв. 1, 2024
The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.
Язык: Английский
Процитировано
13
Deleted Journal, Год журнала: 2024, Номер 2(3)
Опубликована: Май 29, 2024
Abstract The skin interfaces with the external environment, acting as both a physical barrier and an immunologic defense. dynamic interactions between various cell types are essential for homeostasis function. Emerging research has unveiled significant role of extracellular vesicles (EVs) key mediators communication in regulating many aspects physiological pathological processes. In this review, we provide overview recent advances understanding roles EVs health, aging, diseases, discuss natural engineered disease treatment along potential challenges. burgeoning area may expand new therapeutic approaches disorders open avenues personalized skincare treatments.
Язык: Английский
Процитировано
8
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 297, С. 139822 - 139822
Опубликована: Янв. 13, 2025
Язык: Английский
Процитировано
1
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 16, 2025
Chronic diabetic wounds are characterized by prolonged inflammation and excessive accumulation of M1 macrophages, which impede the healing process. Therefore, resolving promptly transitioning to proliferative phase critical steps for effective wound healing. Exosomes have emerged as a promising therapeutic strategy. In this study, smart hydrogel capable responding pathological cues in inflammatory microenvironment promote transition from proliferation delivering M2 macrophage-derived exosomes (M2-Exos) is developed. The synthesized through cross-linking oxidized dextran, matrix metalloproteinase (MMP)-9-sensitive peptide, carboxymethyl chitosan containing M2-Exos. response elevated MMP-9 concentrations microenvironment, demonstrates diagnostic logic, adjusting release kinetics M2-Exos accordingly. on-demand facilitated macrophage polarization phenotype, thereby promoting accelerating transcriptomic analysis further reveals that MMP-9-responsive with delivery exerts anti-inflammatory regenerative effects downregulating inflammation-related pathways. This study introduces an innovative, microenvironment-responsive exosome system enables precise control agent release, offering personalized approach treatment chronic wounds.
Язык: Английский
Процитировано
0
Obesity Medicine, Год журнала: 2025, Номер unknown, С. 100597 - 100597
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Март 6, 2025
Diabetic wound healing remains a significant clinical challenge because of hyperglycaemia-induced cellular senescence, impaired angiogenesis, and chronic inflammation. To address these issues, we developed multifunctional hydrogel (GelMA/PNS/Alg@IGF-1) that integrates gelatine methacryloyl (GelMA), Panax notoginseng saponins (PNS), sodium alginate microspheres encapsulating insulin-like growth factor-1 (IGF-1). This was engineered to achieve gradient sustained release bioactive agents target senescence promote vascular repair. In vitro studies demonstrated the significantly reduced oxidative stress, suppressed markers senescence-associated secretory phenotypes, restored endothelial cell function under high-glucose conditions by inhibiting NF-κB pathway activation. Transcriptomic analysis revealed modulation pathways linked inflammation, apoptosis, angiogenesis. accelerated diabetic closure in rat model vivo enhanced collagen deposition, granulation tissue formation, neovascularization. Furthermore, mitigated stress promoted remodelling. The synergistic effects PNS IGF-1 within established pro-regenerative microenvironment both pathological ageing dysfunction. These findings highlight GelMA/PNS/Alg@IGF-1 as promising therapeutic platform for management, this material offers dual anti-senescence proangiogenic efficacy overcome complexities healing.
Язык: Английский
Процитировано
0
Advanced Materials Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Март 6, 2025
Abstract Extracellular vesicle (EV)‐incorporated hydrogels have emerged as promising scaffolds for tissue repair due to their ability present biological cues. However, the encapsulation efficiency and distribution of EVs within still require improvement enhance healing outcomes. In this study, a novel approach is developed that uses crosslinkers hydrogel formation, ensuring are at every crosslinking point thereby achieving both functional incorporation uniform EVs. Amphiphilic molecules with various groups successfully inserted into EV membrane, enabling macromers, which versatile multiple chemistries. EV‐crosslinked exhibited faster stress relaxation properties stretchability compared crosslinked traditional elastic polymers, promoting enhanced cell spreading proliferation. Additionally, it demonstrated could proteins throughout network while maintaining activity. Using VEGF‐loaded crosslinkers, induced endothelial clustering sprouting successfully, indicating early angiogenic responses. These results underscore potential engineering regenerative medicine, offering tunable mechanical capacity effective protein delivery.
Язык: Английский
Процитировано
0
Molecular and Cellular Biochemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 17, 2025
Язык: Английский
Процитировано
0