Ultrafast enzyme-responsive hydrogel for real-time assessment and treatment optimization in infected wounds

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 8, 2025

Monitoring wound infection and providing appropriate treatment are crucial for achieving favorable outcomes. However, the time-consuming nature of laboratory culture tests may delay timely intervention. To tackle this challenge, a simple yet effective HDG hydrogel, composed hydrogen peroxide (H₂O₂), dopamine, GelMA polymer, is developed ultrafast detection Staphylococcus aureus (SA) infections. The hydrogel detects SA by exploiting its secreted catalase to catalyze H₂O₂, producing oxygen, which in turn accelerates polymerization colorless dopamine into deep brown polydopamine (PDA). bacterial process takes only 10 min with high sensitivity, results can be readily recognized naked eye or quantified using cell phone-based digital analysis. Moreover, provides dual antibacterial mechanism through chemical photothermal therapies via generated PDA, significantly improving clearance. In animal experiments, demonstrated promising capabilities monitoring eliminating bacteria, enhancing collagen deposition, reducing inflammation, promoting healing infected wounds. This multifunctional design offers an enzyme-responsive strategy rapid assessment management infections, simplifying evaluation facilitating development advanced dressings.

Язык: Английский

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Hollow‐Structured Nanorobot with Excellent Magnetic Propulsion for Catalytic Pollutant Degradation, Anti‐Bacterial and Biofilm Removal

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Abstract Chemical pollution, pathogenic bacteria, and bacterial biofilms pose significant threats to public health. Although various nanoplatforms with both catalytic antibacterial activities have been developed, creating a remotely controllable nanorobot precise targeting propulsion capabilities remains challenge. This study presents the fabrication of hollow‐structured Fe 3 O 4 @AgAu@polydopamine (PDA) nanosphere, which demonstrated activity superior magnetically enhanced biofilm removal properties. The AgAu bimetallic nanorods are assembled between core biocompatible PDA, resulting in magnetic high photothermal conversion efficiency (54%) excellent activity. Importantly, due efficient behavior originating from , organic pollutants such as 4‐nitrophenol methylene blue can be accurately degraded by @AgAu@PDA nanorobots simulated wastewater pool. By incorporating zinc phthalocyanine (ZnPc) photosensitizer, @AgAu@PDA‐ZnPc nanosphere exhibits synergistic “photothermal‐photodynamic‐Ag + ” effect against Escherichia coli Staphylococcus aureus . Remarkably, rate 99.99% applying via rotating field (RMF). Furthermore, this unique endows effective flat surfaces tubular structures, highlighting its advantages over traditional agents dynamic applications.

Язык: Английский

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Self‐Activatable Multi‐Module Nanoelectrode Triggering Electro‐Driven Pyroptosis in Cancer Cells Boost Anti‐Tumor Immunity

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Abstract Pyroptosis holds significant promise in facilitating tumor immunotherapy, but its efficiency is hindered by the indiscriminate pyroptotic killing of cells within cancer tissues and inadequate expression gasdermin cells. Herein, a self‐activatable multi‐module nanoelectrode (HMPD) presented, precisely evoking D (GSDMD)‐dependent pyroptosis via GSH‐activatable electrodynamic therapy (EDT) Mn 2+ ‐initiated epigenetic reprogramming. HMPD contains core platinum nanocluster (PtNCs) as electric‐catalytic module cloaked electric‐shield (hyaluronic acid (HA)‐tethered MnO 2 nanoflower, HMNFs), along with interlayer‐location reprogramming (Mn ‐activating DNA Methyltransferase1 (DNMT1) mRNA‐cleavage DNAzymes, DDzymes). Profiting from cloaking HMNFs, an inert material for EDT, activities are silenced. Due to high‐level glutathione (GSH)‐mediated HMNFs dissociation cells, activity awakened through PtNCs exposure, triggering hydroxyl radical (·OH) generation under square‐wave alternating current (AC), enabling EDT‐driven GSDMD‐dependent pyroptosis. Meanwhile, activated action ions‐initiated DDzymes, which unclocked GSDMD preventing DNMT1‐dominated promoter hypermethylation, further sensitizing The cooperation can fully play therapeutic potency pyroptosis, providing alternative approach expand immunotherapy.

Язык: Английский

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Antimicrobial properties and preservation potential of Allium sativum L-derived extracellular vesicle-like particles for food applications

Food Chemistry, Год журнала: 2025, Номер unknown, С. 144419 - 144419

Опубликована: Апрель 1, 2025

Язык: Английский

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