Abstract
Herein,
we
describe
the
design
and
development
of
a
new
cell‐permeable
aggregation‐induced
emission
(AIE)
active
3
‐ethoxysalicylaldimine‐based
symmetrical
azine
molecule
HDBE
.
The
synthesized
compound
underwent
comprehensive
investigation
different
spectroscopic
methods,
like
NMR,
mass
single
crystal
X‐ray
diffraction
analysis.
fluorophore
exhibited
bright
orange
colour
AIE
behaviour
in
THF–H
2
O
mixture.
drastic
enhancement
was
achieved
upon
adding
water
to
THF
solution
,
with
concentration
90%.
Along
dynamic
light
scattering
(DLS)
quantum
yield
measurements,
formation
aggregates
also
verified
by
scanning
electron
microscopy
(SEM)
transmission
(TEM)
Further,
demonstrated
excited
state
intramolecular
proton
transfer
(ESIPT)
characteristics
polarity
solvents,
which
corroborated
absorption,
lifetime
spectroscopical
investigations.
detailed
scrutiny
structure
displayed
two
strong
hydrogen
bonding
interactions,
while
solid‐state
fluorescent
spectra
showed
dual
that
corresponds
enol
keto
form
confirming
ESIPT
feature.
non‐toxic
cell‐permeable,
making
it
easy
label
as
biomarker
live
HeLa
cells
via
bioimaging.
These
studies
offer
quick
way
develop
both
ESIPT‐coupled
molecules
for
cell
bioimaging
applications.
Chemical & Biomedical Imaging,
Год журнала:
2024,
Номер
2(5), С. 331 - 344
Опубликована: Апрель 19, 2024
The
introduction
of
super-resolution
microscopy
(SRM)
has
significantly
advanced
our
understanding
cellular
and
molecular
dynamics,
offering
a
detailed
view
previously
beyond
reach.
Implementing
SRM
in
biophysical
research,
however,
presents
numerous
challenges.
This
review
addresses
the
crucial
aspects
utilizing
effectively,
from
selecting
appropriate
fluorophores
preparing
samples
to
analyzing
complex
data
sets.
We
explore
recent
technological
advancements
methodological
improvements
that
enhance
capabilities
SRM.
Emphasizing
integration
with
other
analytical
methods,
we
aim
overcome
inherent
limitations
expand
scope
biological
insights
achievable.
By
providing
comprehensive
guide
for
choosing
most
suitable
methods
based
on
specific
research
objectives,
empower
researchers
processes
enhanced
precision
clarity,
thereby
advancing
frontiers
research.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 25, 2024
Abstract
Single
luminophore
enabling
stimuli‐responsive
dual‐emission
characteristic
has
received
considerable
interest
in
recent
years
due
to
its
special
advantages
advanced
optical
events.
A
large
number
of
reports
around
the
globe
have
involved
dynamic
or
adjustable
phenomenon
(e.g.,
dual
emission
intensity
ratio)
single‐emitter
based
systems.
However,
a
systematic
overview
summarizing
underlying
mechanisms,
behaviors,
and
applications
this
topic
is,
so
far,
missing.
To
further
promote
development
extend
important
role
such
kind
materials
diverse
communities,
review,
latest
advancements
field
single‐luminophore
that
exhibit
characteristics
are
summarized.
The
discussion
is
centered
on
photophysical
innovative
design
approaches,
strategies
for
modulating
properties,
these
This
review
anticipated
provide
new
insights
into
distinctive
organic
luminescence
community
from
perspective.
ABSTRACT
Fluorescence
quenching,
a
process
where
the
intensity
of
fluorescence
is
diminished
by
various
molecular
interactions,
has
emerged
as
critical
tool
in
drug
discovery.
This
review
delves
into
underlying
mechanisms
including
static
and
dynamic
Förster
resonance
energy
transfer
(FRET),
photoinduced
electron
(PET).
Each
mechanism
offers
unique
insights
binding
affinities,
conformational
changes
candidates,
enabling
researchers
to
dissect
complex
biological
systems
with
precision.
The
application
quenching
high‐throughput
screening
(HTS)
particularly
emphasized,
highlighting
its
role
identifying
lead
compounds
optimizing
drug–target
interactions.
Furthermore,
explores
integration
advanced
techniques,
such
time‐resolved
single‐molecule
spectroscopy,
elucidating
phenomena
at
level.
These
techniques
provide
deeper
understanding
drug–receptor
allosteric
modulation,
protein
dynamics,
which
are
pivotal
development
pipeline.
potential
probing
pharmacokinetics
pharmacodynamics
novel
therapeutics
also
discussed,
underscoring
versatility
effectiveness.
By
offering
comprehensive
analysis
their
applications,
this
aims
inform
future
discovery
endeavors,
fostering
more
effective
targeted
therapies.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(49)
Опубликована: Авг. 16, 2024
Abstract
Selective
detection
of
reactive
oxygen
species
(ROS)
is
vital
for
studying
their
role
in
brain
diseases.
Fluorescence
probes
can
distinguish
ONOO
−
from
other
ROS;
however,
selectivity
toward
depends
on
the
recognition
group.
Aryl‐boronic
acids
and
esters,
which
are
common
groups,
not
selective
over
H
2
O
.
In
this
study,
we
developed
a
diaminonaphthalene
(DAN)‐protected
boronic
acid
as
new
group
that
selectively
reacts
with
ROS.
Three
DAN‐protected
(DANBA)‐based
fluorophores
emit
fluorescence
visible
to
near‐infrared
(NIR)
regions,
Cou‐BN
,
BVP‐BN
HDM‐BN
aryl‐boronic
acid‐based
counterparts
(
Cou‐BO
BVP‐BO
HDM‐BO
),
were
developed.
The
DANBA‐based
exhibited
enhanced
control
group,
well
universality
solution
assays
vitro
experiments
PC12
cells.
NIR‐emissive
was
optimized
delineate
vivo
levels
mouse
brains
Parkinson's
disease.
This
belongs
generation
groups
developing
probes,
strategy
could
be
extended
hydroxyl‐containing
dyes
detect
complex
biological
systems
processes.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Molecular
sensors
respond
to
the
presence
of
biological
analytes
by
producing
signals
that
are
either
directly
perceivable
human
sensory
systems
or
converted
into
electric
signals,
which
require
electronic
devices
for
communicating
humans.
Here,
we
review
outputs
molecular
detectable
senses.
According
literature,
with
visual
dominate.
Undeservedly
unnoticed,
release
gases
might
be
particularly
useful
since
gas
output
can
detected
several
senses
in
a
quantifiable
format.
Relatively
new
tactile
accessed
visually
impaired
people.
their
bypassing
may
contribute
development
point-of-care
testing
technologies,
as
well
providing
direct
communication
nanorobots
