Abstract
Herein,
we
describe
the
design
and
development
of
a
new
cell‐permeable
aggregation‐induced
emission
(AIE)
active
3
‐ethoxysalicylaldimine‐based
symmetrical
azine
molecule
HDBE
.
The
synthesized
compound
underwent
comprehensive
investigation
different
spectroscopic
methods,
like
NMR,
mass
single
crystal
X‐ray
diffraction
analysis.
fluorophore
exhibited
bright
orange
colour
AIE
behaviour
in
THF–H
2
O
mixture.
drastic
enhancement
was
achieved
upon
adding
water
to
THF
solution
,
with
concentration
90%.
Along
dynamic
light
scattering
(DLS)
quantum
yield
measurements,
formation
aggregates
also
verified
by
scanning
electron
microscopy
(SEM)
transmission
(TEM)
Further,
demonstrated
excited
state
intramolecular
proton
transfer
(ESIPT)
characteristics
polarity
solvents,
which
corroborated
absorption,
lifetime
spectroscopical
investigations.
detailed
scrutiny
structure
displayed
two
strong
hydrogen
bonding
interactions,
while
solid‐state
fluorescent
spectra
showed
dual
that
corresponds
enol
keto
form
confirming
ESIPT
feature.
non‐toxic
cell‐permeable,
making
it
easy
label
as
biomarker
live
HeLa
cells
via
bioimaging.
These
studies
offer
quick
way
develop
both
ESIPT‐coupled
molecules
for
cell
bioimaging
applications.
Chemical & Biomedical Imaging,
Год журнала:
2024,
Номер
2(5), С. 331 - 344
Опубликована: Апрель 19, 2024
The
introduction
of
super-resolution
microscopy
(SRM)
has
significantly
advanced
our
understanding
cellular
and
molecular
dynamics,
offering
a
detailed
view
previously
beyond
reach.
Implementing
SRM
in
biophysical
research,
however,
presents
numerous
challenges.
This
review
addresses
the
crucial
aspects
utilizing
effectively,
from
selecting
appropriate
fluorophores
preparing
samples
to
analyzing
complex
data
sets.
We
explore
recent
technological
advancements
methodological
improvements
that
enhance
capabilities
SRM.
Emphasizing
integration
with
other
analytical
methods,
we
aim
overcome
inherent
limitations
expand
scope
biological
insights
achievable.
By
providing
comprehensive
guide
for
choosing
most
suitable
methods
based
on
specific
research
objectives,
empower
researchers
processes
enhanced
precision
clarity,
thereby
advancing
frontiers
research.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 25, 2024
Abstract
Single
luminophore
enabling
stimuli‐responsive
dual‐emission
characteristic
has
received
considerable
interest
in
recent
years
due
to
its
special
advantages
advanced
optical
events.
A
large
number
of
reports
around
the
globe
have
involved
dynamic
or
adjustable
phenomenon
(e.g.,
dual
emission
intensity
ratio)
single‐emitter
based
systems.
However,
a
systematic
overview
summarizing
underlying
mechanisms,
behaviors,
and
applications
this
topic
is,
so
far,
missing.
To
further
promote
development
extend
important
role
such
kind
materials
diverse
communities,
review,
latest
advancements
field
single‐luminophore
that
exhibit
characteristics
are
summarized.
The
discussion
is
centered
on
photophysical
innovative
design
approaches,
strategies
for
modulating
properties,
these
This
review
anticipated
provide
new
insights
into
distinctive
organic
luminescence
community
from
perspective.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Molecular
sensors
respond
to
the
presence
of
biological
analytes
by
producing
signals
that
are
either
directly
perceivable
human
sensory
systems
or
converted
into
electric
signals,
which
require
electronic
devices
for
communicating
humans.
Here,
we
review
outputs
molecular
detectable
senses.
According
literature,
with
visual
dominate.
Undeservedly
unnoticed,
release
gases
might
be
particularly
useful
since
gas
output
can
detected
several
senses
in
a
quantifiable
format.
Relatively
new
tactile
accessed
visually
impaired
people.
their
bypassing
may
contribute
development
point-of-care
testing
technologies,
as
well
providing
direct
communication
nanorobots
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 25, 2025
Acylaminoacyl-peptide
hydrolase
(APEH),
a
serine
peptidase
that
belongs
to
the
prolyl
oligopeptidase
(POP)
family,
catalyzes
removal
of
N-terminal
acetylated
amino
acid
residues
from
peptides.
As
key
regulator
protein
acetylation,
APEH
was
involved
in
many
important
physiological
processes
while
its
aberrant
expression
correlated
with
progression
various
diseases
such
as
inflammation,
diabetics,
Alzheimer's
disease
(AD),
and
cancers.
However,
emerging
attention
has
been
attracted
APEH-related
diagnosis
drug
discovery,
mechanisms
behind
related
are
still
unclear;
thus,
further
investigating
role
function
is
great
importance.
To
date,
enzyme-activated
fluorescent
probes
targeting
POPs
have
extensively
reported
adopted
relevant
medical
research
applications.
Nevertheless,
an
member
POP
rarely
referred
field
bioimaging
probe
for
vivo
sensing
activity
not
yet.
Thus,
acquiring
efficient
APEH-targeted
urgent
need.
Herein,
fluorogenic
profiling
first
discovered
via
substrate
mimic-based
strategy.
By
combination
stimulated
molecular
docking-based
preliminary
screening
experiment-based
secondary
screening,
optimal
(named
TMN-AcA),
which
displayed
high
binding
affinity,
sensitivity,
specificity
toward
APEH,
screened
out.
Owing
superior
properties
TMN-AcA,
endogenous
cell
lines
transplanted
tumor
could
be
visualized
tissue
distribution
revealed.
Most
importantly,
demonstrated
potential
biomarker
multiple-organ
injury
TMN-AcA-based
immunohistochemistry
(IHC)
analysis
newly
developed
serve
vital
tool
biological
study.
