New AIE Emitters from the Unexpected Boron Tribromide/Boron Trichloride‐mediated Cyclization Reaction and Application for Fluorescence Imaging of Lipid Droplets

Aggregate, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

ABSTRACT The aberrant behavior of lipid droplets (LDs) is often indicative cellular dysfunction, which may contribute to the development a range diseases, particularly metabolic dysfunction‐associated steatotic liver disease (MASLD) and atherosclerosis (AS). Consequently, there an urgent need develop fluorescence probes targeting LDs monitor progression disease. In this study, unanticipated one‐pot boron tribromide (BBr₃)/boron trichloride (BCl₃)‐promoted cyclization reaction was discovered, yielding bromo‐/chloro‐substituted triphenylamine (TPA) derivative ( TPA‐Br / TPA‐Cl ). successfully transformed into new TPA‐containing donor‐acceptor (D–A) molecules show typical aggregation induced emission (AIE) property. Among these AIE emitters, TPA‐N shows most promising specificity, lowest toxicity best photo‐stability. Ex vivo studies further demonstrate that can be used image fatty AS plaque quickly effectively.

Язык: Английский

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Macrocyclic Peptide-Based Dual-Sensor Platform for Linkage-Specific Visualization of Ubiquitin Chain Assembling in Live Cells

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Intracellular monitoring of protein ubiquitination and differentiating polyubiquitin chain topology are crucial for understanding life processes drug discovery, which is challenged by the high complexity process a lack molecular tools. Herein, synthetic dual-sensor platform specific K48-linked ubiquitin oligomers was tailored in situ visualization assembling live biosystems. This achieved using macrocyclic peptides as recognition motifs tetraphenylethylene derivative an activatable reporter. The efficient cell penetration, tight binding, protection delivered "freeze-and-image" approach, allowing fluorescent readout linkage type elongation without perturbing physiological environment. Motivated these unique features, mapping K48-ubiquitination dynamics during degradation facilely achieved. Rapid, sensitive, intracellular assessment mechanism action potency dependence proteolysis-targeting chimeras (PROTACs) demonstrated, presenting sensors promising tools PROTAC development.

Язык: Английский

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Tetraphenylethene‐Based cis/trans Isomers for Targeted Fluorescence Sensing and Biomedical Applications

Chemistry - A European Journal, Год журнала: 2023, Номер 29(30)

Опубликована: Март 21, 2023

Molecular probes which can be modulated, functionalized and used to visualize the processes are highly desirable for understanding manipulating biological systems. Geometric cis trans isomers of tetraphenylethene (TPE) emerge as attractive candidates fulfill these tasks thanks unique aggregation-induced emission properties, tailorable structures, responsiveness external stimuli. This minireview focuses on TPE derivatives that with molecular recognition units fluorescence detection, bioimaging cancer therapy. The effects geometry property, target binding ability activity summarized. feasibility in vitro vivo switch configuration thus bio-activity is discussed. Finally, future development challenges discussed view TPE-based stereoisomers targeted sensing imaging-guided modulation processes.

Язык: Английский

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A self-assembled nanophotosensitizer targets lysosomes and induces lysosomal membrane permeabilization to enhance photodynamic therapy

Chemical Science, Год журнала: 2023, Номер 14(19), С. 5106 - 5115

Опубликована: Янв. 1, 2023

Self-assembly of amphiphilic photosensitizer BDQ into BDQ-NP enables stable incorporation lysosome membranes to cause continuous lysosomal membrane permeabilization for highly effective photodynamic therapy.

Язык: Английский

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Synthetic Peptides with Genetic‐Codon‐Tailored Affinity for Assembling Tetraspanin CD81 at Cell Interfaces and Inhibiting Cancer Metastasis

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(20)

Опубликована: Фев. 27, 2024

Probing biomolecular interactions at cellular interfaces is crucial for understanding and interfering with life processes. Although affinity binders site specificity membrane proteins are unparalleled molecular tools, a high demand remains novel multi-functional ligands. In this study, synthetic peptide (APQQ) tight specific binding to the untargeted extracellular loop of CD81 evolved from genetically encoded pool. With tailored affinity, APQQ flexibly accesses, site-specifically binds, forms complex CD81, enabling in-situ tracking dynamics activity protein in living cells, which has rarely been explored because lack Furthermore, triggers relocalization diffuse densely clustered cell junctions modulates interplay interfaces. Motivated by these, efficient suppression cancer migration, inhibition breast metastasis were achieved vivo.

Язык: Английский

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Targeting Sub-Cellular Organelles for Boosting Precision Photodynamic Therapy

Chemical Communications, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Among various cancer treatment methods, photodynamic therapy has received significant attention due to its non-invasiveness and high efficiency in inhibiting tumour growth. Recently, specific organelle targeting photosensitizers have increasing interest their precise accumulation ability trigger organelle-mediated cell death signalling pathways, which greatly reduces the drug dosage, minimizes toxicity, avoids multidrug resistance, prevents recurrence. In this review, recent advances representative used targeted on organelles, specifically including endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, lysosomes, been comprehensively reviewed with a focus structure pathways. Furthermore, perspective future research potential challenges precision presented at end.

Язык: Английский

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Peptide-derived coordination frameworks for biomimetic and selective separation

Analytical and Bioanalytical Chemistry, Год журнала: 2023, Номер 415(18), С. 4079 - 4092

Опубликована: Май 26, 2023

Язык: Английский

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Compact Micropatterned Chip Empowers Undisturbed and Programmable Drug Addition in High‐Throughput Cell Screening

Advanced Materials, Год журнала: 2023, Номер 36(11)

Опубликована: Окт. 5, 2023

Abstract Simultaneously adding multiple drugs and other chemical reagents to individual droplets at specific time points presents a significant challenge, particularly when dealing with tiny in high‐throughput screening applications. In this study, micropatterned polymer chip is developed as miniaturized platform for light‐induced programmable drug addition cell‐based screening. This incorporates porous superhydrophobic film atom transfer radical polymerization reactivity, facilitating the efficient grafting of azobenzene methacrylate, photoconformationally changeable group, onto hydrophilic regions matrix targeted locations precise densities. By employing light irradiation, cyclodextrin‐azobenzene host–guest complexes formed on can switch from an “associated” “dissociated” state, granting photochemical control over supramolecular coding system its surface patterning ability. Significantly, exceptional spatial temporal offered by these transitions empowers utilize digital processing systems simultaneous regulation release cyclodextrin‐bearing across numerous containing suspended or adhered cells. approach minimizes mechanical disruption while achieving timing addition, dosage, integration varieties released screening, all meet requirements.

Язык: Английский

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Bioinspired Chemosensor with Configuration-Dependent Affinity for Localized Tracking of Labile Zn²⁺ during Neuronal Differentiation

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

Homeostasis and transportation of labile Zn2+ across neuronal membranes are great importance for biological functions, in which molecular tools urgently demanded to understand the mechanism actions depth. Here, we report a zinc-specific fluorescence-switchable chemosensor (trans T-P) targetable tracing live nerve cells. The design was inspired by coordination structure catalytic centers metalloenzymes. strong response were demonstrated be stereochemistry-sensitive, only trans conformer has ultrahigh affinity, selectivity, sensitivity. Benefited from its amphiphilic phosphate-bearing structure, T-P is readily cell membrane permeable achieves preferential accumulation near-membrane region, enabling regionally specific localization imaging at intracellular side plasma membranes. Provided with these unique properties, achieved an situ visualization fluctuations during differentiation. targeted sorting tracking highlight prominence monitoring spatiotemporal dynamics understanding functions development.

Язык: Английский

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Molecularly Targeted Fluorescent Sensors for Visualizing and Tracking Cellular Senescence

Biosensors, Год журнала: 2023, Номер 13(9), С. 838 - 838

Опубликована: Авг. 23, 2023

Specific identification and monitoring of senescent cells are essential for the in-depth understanding regulation senescence-related life processes diseases. Fluorescent sensors providing real-time in situ information with spatiotemporal resolution unparalleled tools have contributed greatly to this field. This review focuses on recent progress fluorescent molecularly targeted imaging tracking cellular senescence. The molecular design, sensing mechanisms, biological activities discussed. categorized by types markers targeting ligands. Accordingly, their recognition performance towards senescence biomarkers summarized. Finally, perspective challenges field discussed, which expected assist future design next-generation

Язык: Английский

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