Angewandte Chemie,
Год журнала:
2023,
Номер
136(1)
Опубликована: Ноя. 17, 2023
Abstract
Fluorescent
probes
are
essential
for
single‐molecule
imaging.
However,
their
application
in
biological
systems
is
often
limited
by
the
short
photobleaching
lifetime.
To
overcome
this,
we
developed
a
novel
thiolation
strategy
squaraine
dyes.
By
introducing
of
central
cyclobutene
(thio‐squaraine),
observed
≈5‐fold
increase
Our
data
analysis
attributes
this
improvement
to
improved
photostability
resulting
from
thiolation.
Interestingly,
bulk
measurements
show
rapid
oxidation
thio‐squaraine
its
oxo‐analogue
under
irradiation,
giving
perception
inferior
photostability.
This
discrepancy
between
and
environments
can
be
ascribed
factors
latter,
including
larger
intermolecular
distances
restricted
mobility,
which
reduce
interactions
fluorophore
reactive
oxygen
species
produced
other
fluorophores,
ultimately
impacting
photoconversion
rate.
We
demonstrate
remarkable
performance
various
imaging
buffers,
such
as
glucose
oxidase
with
catalase
(GLOX)
GLOX+trolox.
successfully
employed
these
photostable
tracking
CD56
membrane
protein
monitoring
mitochondria
movements
live
neurons.
revealed
distinct
motion
states
corresponding
fractions.
investigation
expected
propel
development
probes,
particularly
scenarios
where
suboptimal
performance.
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(25), С. 17456 - 17473
Опубликована: Июнь 11, 2024
Photomodulable
fluorescent
probes
are
drawing
increasing
attention
due
to
their
applications
in
advanced
bioimaging.
Whereas
photoconvertible
can
be
advantageously
used
tracking,
photoswitchable
constitute
key
tools
for
single-molecule
localization
microscopy
perform
super-resolution
imaging.
Herein,
we
shed
light
on
a
red
and
far-red
BODIPY,
namely,
BDP-576
BDP-650,
which
possess
both
properties
of
conversion
switching.
Our
study
demonstrates
that
these
pyrrolyl-BODIPYs
convert
into
typical
green-
red-emitting
BODIPYs
perfectly
adapted
microscopy.
We
also
showed
this
undergo
Directed
Photooxidation
Induced
Conversion,
photoconversion
mechanism
recently
introduced,
where
the
pyrrole
moiety
plays
central
role.
These
unique
features
were
develop
targeted
toward
different
organelles
or
subcellular
units
(plasma
membrane,
mitochondria,
nucleus,
actin,
Golgi
apparatus,
etc.)
using
chemical
targeting
moieties
Halo
tag.
notably
BDP-650
could
track
intracellular
vesicles
over
more
than
20
min
two-color
imagings
with
laser
scanning
confocal
microscopy,
demonstrating
its
robustness.
The
switching
photoconverters
studied
at
level
then
successfully
live
epithelial
cells
neurons.
Both
membrane-
mitochondria-
decipher
membrane
3D
architecture
mitochondrial
dynamics
nanoscale.
This
builds
bridge
between
photoswitching
undergoing
directed
photooxidation
shows
versatility
efficacy
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 2, 2025
Bioorthogonal
chemistry-mediated
self-assembly
holds
great
promise
for
dynamic
molecular
imaging
in
living
organisms.
However,
existing
approaches
are
limited
to
nanoaggregates
with
'always-on'
signals,
suffering
from
high
signal-to-background
ratio
(SBR)
and
compromised
detection
sensitivity.
Herein
we
report
a
nitrile-aminothiol
(NAT)
bioorthogonal
fluorogenic
probe
(CyNAP-SS-FK)
ultrasensitive
diagnosis
of
orthotopic
hepatocellular
carcinoma.
This
comprises
nitrile-substituted
hemicyanine
scaffold
cysteine
tail
dually
locked
biomarker-responsive
moieties.
Upon
dual
cleavage
by
tumor-specific
cathepsin
B
biothiols,
the
1,2-aminothiol
residue
is
exposed
spontaneously
reacts
nitrile
group
situ
intramolecular
macrocyclization,
enabling
near-infrared
fluorescence
(NIRF)
turn-on
as
well
self-assembly.
In
male
mice,
such
'cleavage-click-assembly'
regimen
allows
real-time
small
cancerous
lesions
(~2
mm
diameter)
improved
SBR
(~5)
extended
window
(~36
h),
outperforming
conventional
clinical
assays.
study
not
only
presents
NAT
click
reaction-based
probes
but
also
highlights
generic
dual-locked
design
these
probes.
Existing
can
suffer
low
Here,
authors
sensing
action
lesions.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
63(1)
Опубликована: Ноя. 17, 2023
Fluorescent
probes
are
essential
for
single-molecule
imaging.
However,
their
application
in
biological
systems
is
often
limited
by
the
short
photobleaching
lifetime.
To
overcome
this,
we
developed
a
novel
thiolation
strategy
squaraine
dyes.
By
introducing
of
central
cyclobutene
(thio-squaraine),
observed
≈5-fold
increase
Our
data
analysis
attributes
this
improvement
to
improved
photostability
resulting
from
thiolation.
Interestingly,
bulk
measurements
show
rapid
oxidation
thio-squaraine
its
oxo-analogue
under
irradiation,
giving
perception
inferior
photostability.
This
discrepancy
between
and
environments
can
be
ascribed
factors
latter,
including
larger
intermolecular
distances
restricted
mobility,
which
reduce
interactions
fluorophore
reactive
oxygen
species
produced
other
fluorophores,
ultimately
impacting
photoconversion
rate.
We
demonstrate
remarkable
performance
various
imaging
buffers,
such
as
glucose
oxidase
with
catalase
(GLOX)
GLOX+trolox.
successfully
employed
these
photostable
tracking
CD56
membrane
protein
monitoring
mitochondria
movements
live
neurons.
revealed
distinct
motion
states
corresponding
fractions.
investigation
expected
propel
development
probes,
particularly
scenarios
where
suboptimal
performance.
Single-molecule
tracking
offers
nanometer
resolution
for
studying
individual
molecule
dynamics
but
is
often
limited
by
sparse
labeling
to
avoid
signal
overlap.
We
present
Red-Light-Activated
Tracking
(RE-LAST)
strategy
address
this
challenge
utilizing
a
photoactivatable
probe,
SiR670.
SiR670
combines
traditional
silicon
rhodamine
with
photocage
called
SO,
quenching
fluorescence
via
photoinduced
electron
transfer
(PET).
Red
light
triggers
excitation,
generating
singlet
oxygen
that
oxidizes
the
SO
cage,
halting
PET
and
restoring
fluorescence.
RE-LAST
used
red
both
activation
imaging,
eliminating
harmful
UV
exposure.
This
method
enables
high-throughput
single-molecule
tracking,
achieving
approximately
9
times
more
tracks
than
conventional
methods
allowing
detailed
classification
of
CD56
membrane
protein
motion.
Furthermore,
in
situ
imaging
single
live
cells
revealed
effects
triplet
quencher
scavenging
system
(OSS)
on
dynamics.
While
quenchers
like
Trolox
had
minimal
impact
movement
patterns,
OSS
significantly
accelerated
increased
proportion
mobile
proteins.
approach
provides
comprehensive
investigating
living
cells,
contributing
further
developments
cellular
molecular
biology.
ACS Macro Letters,
Год журнала:
2025,
Номер
unknown, С. 757 - 764
Опубликована: Май 20, 2025
Single
gas
donors
have
proved
to
vast
therapeutic
potential;
however,
the
investigation
of
multigas
releasing
molecules
is
limited.
Besides,
porphyrins
and
their
derivatives
are
essential
energy
electron
transfer
agents
for
activating
gaseous
signaling
molecule
(GSM)
donors,
despite,
catalysis
latter
greatly
limited
in
oxygen-rich
environments.
We,
therefore,
report
photosensitive
small-molecule
hybrids
composed
oxo-/thio-flavonols
as
singlet
oxygen
(1O2)
scavengers
catalytic
stability
N-nitrosoamine
moieties
competent
co-release
carbon
monoxide
(CO)
nitric
oxide
(NO)
amounts
that
depend
on
structural
designs.
The
self-assembled
micellar
co-encapsulation
CO/NO
PdTPTBP
afforded
intracellular
GSMs
delivery
during
red-light
irradiation.
bactericidal
effect
irradiated
micelles
against
planktonic
Gram-positive
Gram-negative
bacteria
displayed
a
significant
killing
rate
S.
aureus
via
membrane
hyperpolarization
rupture
achieved
an
excellent
combination
index
0.44.
This
represents
high
potential
biomedical
applications.
Chemistry - A European Journal,
Год журнала:
2023,
Номер
29(20)
Опубликована: Янв. 31, 2023
Dual-emissive
photoconvertible
fluorophores
(DPCFs)
are
powerful
tools
to
unambiguously
track
labeled
cells
in
bioimaging.
We
recently
introduced
a
new
rational
mechanism
called
directed
photooxidation-induced
conversion
(DPIC)
enabling
efficient
DPCFs
be
obtained
by
conjugating
coumarin
aromatic
singlet-oxygen
reactive
moieties
(ASORMs).
Pyrrole
was
found
suitable
ASORM
as
it
provided
high
hypsochromic
shift
along
with
fast
and
conversion.
By
synthesizing
various
pyrrole-based
styryl
dyes,
we
showed
that
the
photoconversion
properties,
including
quantum
yield
of
chemical
can
tuned
modification
pyrrole.
These
modifications
led
an
improved
dual
emissive
converter,
SCP-Boc,
which
displayed
brightness
enhanced
63
%.
SCP-Boc
successfully
used
sequentially
photoconvert
laser
scanning
confocal
microscopy.
