DNA Origami – Lipid Membrane Interactions Defined at Single-Molecular Resolution DOI Creative Commons
Elena Georgiou, Javier Cabello-García, Yongzheng Xing

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 14, 2023

ABSTRACT Rigid DNA nanostructures that bind to floppy bilayer membranes are of fundamental interest as they replicate biological cytoskeletons for synthetic biology, biosensing, and research. Here, we establish principles underpinning the controlled interaction structures lipid bilayers. As membrane anchors mediate interaction, more than 20 versions a core nanostructure built each carrying up five individual cholesterol different steric accessibility within 3D geometry. The structures’ binding vesicles tunable curvature is determined with ensemble methods by single-molecule localization microscopy. This screen yields quantitative unexpected insight on which anchor points cause efficient binding. Strikingly, defined single molecular discriminate effectively between nanoscale curvatures may be exploited discern diagnostically relevant based size. Furthermore, reveal anchor-mediated co-controlled non-lipidated regions localized stemming from heterogenous composition, modifies existing biophysical models. Our study extends nanotechnology control interactions thereby facilitate design nanodevices vesicle-based diagnostics, protocells.

Язык: Английский

Lipid vesicle-based molecular robots DOI Creative Commons
Zugui Peng,

Shoji Iwabuchi,

Kayano Izumi

и другие.

Lab on a Chip, Год журнала: 2024, Номер 24(5), С. 996 - 1029

Опубликована: Янв. 1, 2024

A molecular robot, which is a system comprised of one or more machines and computers, can execute sophisticated tasks in many fields that span from nanomedicine to green nanotechnology. The core parts robots are fairly consistent always include (i) body encapsulate machines, (ii) sensors capture signals, (iii) computers make decisions, (iv) actuators perform tasks. This review aims provide an overview approaches considerations develop robots. We first introduce the basic technologies required for constructing robots, describe recent progress towards achieving higher functionality, subsequently discuss current challenges outlook. also highlight applications sensing biomarkers, signal communications with living cells, conversion energy. Although still their infancy, they will unquestionably initiate massive change biomedical environmental technology not too distant future.

Язык: Английский

Процитировано

10
Compliant DNA Origami Nanoactuators as Size‐Selective Nanopores DOI Creative Commons
Ze Yu,

Anna V. Baptist,

S. Reinhardt

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Июль 16, 2024

Biological nanopores crucially control the import and export of biomolecules across lipid membranes in cells. They have found widespread use biophysics biotechnology, where their typically narrow, fixed diameters enable selective transport ions small molecules, as well DNA peptides for sequencing applications. Yet, due to channel sizes, they preclude passage large macromolecules, e.g., therapeutics. Here, unique combined properties origami nanotechnology, machine-inspired design, synthetic biology are harnessed, present a structurally reconfigurable MechanoPore (MP) that features lumen is tuneable size through molecular triggers. Controllable switching MPs between 3 stable states confirmed by 3D-DNA-PAINT super-resolution imaging dye-influx assays, after reconstitution membrane liposomes via an inverted-emulsion cDICE technique. Confocal transmembrane shows size-selective behavior with adjustable thresholds. Importantly, conformational changes fully reversible, attesting robust mechanical overcomes pressure from surrounding molecules. These advance nanopore technology, offering functional nanostructures can be tuned on-demand - thereby impacting fields diverse drug delivery, biomolecule sorting, sensing, bottom-up biology.

Язык: Английский

Процитировано

4
Ions in Motion: From Biological Channels to Engineered Transport Systems DOI Creative Commons
Bei Wu,

Yifan Yan,

Yuting Zhu

и другие.

Giant, Год журнала: 2025, Номер unknown, С. 100352 - 100352

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
DNAzyme-Assisted the Detection of rps27l mRNA in Protein Nanopores DOI
Kang Luo, Xu Wang,

Ling Zheng

и другие.

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 343711 - 343711

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transport DOI Creative Commons
Xiaoming Liu, Fengyu Liu, Hemani Chhabra

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 22, 2024

Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, synthetic cells. Lumen-tunable nanopore like the natural ion channels systematically increasing lumen size have become long-standing desires developing nanopores. Here, we design a triangular with large tunable lumen. It allows in-situ transition from expanded state contracted without changing its stable shape, vice versa, which specific bindings as stimuli mechanically pinch release three corners frame. Transmission electron microscopy images dynamics simulations illustrate architectures high shape retention. Single-channel current recordings fluorescence influx studies demonstrate low-noise repeatable readouts controllable cross-membrane macromolecular transport. We envision that proposed could offer powerful tools sensing, drug delivery, creation Dynamic for various biomedical applications. authors report on can switch between states provide signals sensing.

Язык: Английский

Процитировано

2
Advances in miniaturized nanosensing platforms for analysis of pathogenic bacteria and viruses DOI
Abdallah M. Zeid, Islam M. Mostafa,

Baohua Lou

и другие.

Lab on a Chip, Год журнала: 2023, Номер 23(19), С. 4160 - 4172

Опубликована: Янв. 1, 2023

Integration of smart miniaturized nanosensors with artificial intelligence results in precise detection pathogenic bacteria or viruses biological samples.

Язык: Английский

Процитировано

5
Artificial Molecular Systems for Complex Functions Based on DNA Nanotechnology and Cell‐Sized Lipid Vesicles DOI Creative Commons
Yusuke Sato

ChemSystemsChem, Год журнала: 2024, Номер 6(4)

Опубликована: Май 11, 2024

Abstract Cells are highly functional and complex molecular systems. Artificially creating such systems remains a challenge, which has been extensively studied in various research fields, including synthetic biology robotics. DNA nanotechnology is powerful tool for bottom‐up engineering constructing nanostructures or chemical reaction networks can be utilized as components artificial Encapsulation of these into giant unilamellar vesicle (GUV) composed lipid bilayer, the base structure cellular membrane, results cell‐sized that partially mimics some functions. This review discusses studies contributing to construction GUV‐based based on nanotechnology. Molecular transport signal transduction through membranes essential uptake molecules from environment respond stimuli. Membrane shaping relates functions, motility signaling. A network required autonomously regulate system‘s describes functions realized using networks. Given designability programmability nanotechnology, it may possible functionality could comparable even surpass natural

Язык: Английский

Процитировано

1
A dynamically gated triangular DNA nanopore for molecular sensing and cross-membrane transport DOI Creative Commons
Xiaoming Liu, Fengyu Liu, Hemani Chhabra

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Фев. 1, 2024

Abstract Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, synthetic cells. Dynamically gating cargo like the natural ion channels systematically increasing lumen size have become long-standing desires developing nanopores. Here, we design a triangular nanopore with large dynamically-gated lumen. It can switch between expanded contracted states without changing its stable shape, whereby specific bindings as stimuli mechanically pinch release three corners frame. Transmission electron microscopy images dynamics simulations illustrated up 539 nm2, architectures, high shape retention. Single-channel current recordings fluorescence influx studies demonstrated low-noise repeatable readouts controllable cross-membrane macromolecular transport. We envision that proposed could offer powerful tools sensing, drug delivery, creation

Язык: Английский

Процитировано

0
DNAナノチャネルの高効率膜挿入手法の開発 DOI Open Access
Hiromu Akai,

Hiroki Koiwa,

Yukihiro Izawa

и другие.

Seibutsu Butsuri, Год журнала: 2024, Номер 64(1), С. 28 - 31

Опубликована: Янв. 1, 2024

DNAナノチャネルは,緻密な配列設計や化学修飾により,チャネルタンパク質の機能模倣に成功している一方で,その膜挿入効率は低いため,応用展開は制限されている.本稿では,DNAナノチャネルを物理的に膜挿入することで,膜挿入効率を従来の3倍に向上させた「DNAナノチャネルプローブ技術」について紹介する.

Процитировано

0
Compliant DNA Origami Nanoactuators as Size-Selective Nanopores DOI Open Access
Ze Yu,

Anna V. Baptist,

S. Reinhardt

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 15, 2024

ABSTRACT Biological nanopores crucially control the import and export of biomolecules across lipid membranes in cells. They have found widespread use biophysics biotechnology, where their typically narrow, fixed diameters enable selective transport ions small molecules as well DNA peptides for sequencing applications. Yet, due to channel sizes, they preclude passage large macromolecules, e.g., therapeutics. Here, we harness unique combined properties origami nanotechnology, machine-inspired design, synthetic biology, present a structurally reconfigurable MechanoPore (MP) that features lumen is tuneable size through molecular triggers. Controllable switching MPs between three stable states confirmed by 3D-DNA-PAINT super-resolution imaging dye-influx assays, after reconstitution membrane liposomes via an inverted-emulsion cDICE technique. Confocal transmembrane shows size-selective behaviour with adjustable thresholds. Importantly, conformational changes are fully reversible, attesting robust mechanical overcomes pressure from surrounding molecules. These advance nanopore technology, offering functional nanostructures can be tuned on-demand – thereby impacting fields diverse drug-delivery, biomolecule sorting sensing, bottom-up biology.

Язык: Английский

Процитировано

0