Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 27, 2024
Mucosal-associated
invariant
T
(MAIT)
cells
exhibit
significant
potential
in
the
assessment
of
tumor
development
and
immunotherapy.
However,
there
is
currently
no
convenient
efficient
method
to
analyze
quantitative
changes
MAIT
during
cancer
treatment,
which
has
not
been
extensively
studied.
Here,
we
report
an
electrochemical
biosensor
designed
efficiently
monitor
peripheral
blood
by
simultaneously
recognizing
Vα7.2
CD161
on
cells.
Natural
red
cell
membrane,
tetrahedral
DNA
nanostructure,
modified
nanometal
framework
are
selected
as
antifouling
coating,
antibody
scaffold,
probe,
respectively.
Owing
synergistic
effects
these
materials,
achieves
robust
ability
while
maintaining
excellent
detection
performance
using
rapid
differential
pulse
voltammetry.
We
show
a
decrease
number
associated
with
aging
mucosa-associated
tumors.
Our
research
prospects
assessing
degree
malignancy
tumors,
distinguishing
immunotherapy
responses
patients,
reducing
costs,
promoting
transformation
sensing
technology
into
clinical
settings.
Abstract
Cancer
cells
typically
display
redox
imbalance
compared
with
normal
due
to
increased
metabolic
rate,
accumulated
mitochondrial
dysfunction,
elevated
cell
signaling,
and
accelerated
peroxisomal
activities.
This
may
regulate
gene
expression,
alter
protein
stability,
modulate
existing
cellular
programs,
resulting
in
inefficient
treatment
modalities.
Therapeutic
strategies
targeting
intra‐
or
extracellular
states
of
cancer
at
varying
state
progression
trigger
programmed
death
if
exceeded
a
certain
threshold,
enabling
therapeutic
selectivity
overcoming
resistance
radiotherapy
chemotherapy.
Nanotechnology
provides
new
opportunities
for
modulating
their
excellent
designability
high
reactivity.
Various
nanomaterials
are
widely
researched
enhance
highly
reactive
substances
(free
radicals)
production,
disrupt
the
endogenous
antioxidant
defense
systems,
both.
Here,
physiological
features
described
challenges
illustrated.
Then,
that
classified
elaborated
upon
based
on
ability
target
regulations.
Finally,
future
perspectives
this
field
proposed.
It
is
hoped
review
guidance
design
nanomaterials‐based
approaches
involving
therapy,
especially
cancers
resistant
chemotherapy,
etc.
Proceedings of the National Academy of Sciences,
Год журнала:
2025,
Номер
122(12)
Опубликована: Март 20, 2025
Simultaneous
profiling
of
multiple
molecules
trafficking
at
a
single
organelle
and
the
surrounding
cytosol
within
living
cell
is
crucial
for
elucidating
their
functions,
necessitating
advanced
techniques
that
provide
high
spatial–temporal
resolution
molecule
specificity.
In
this
study,
we
present
an
electrochemical
nanodevice
based
on
θ-nanopipette
designed
to
coanalyze
calcium
ions
(Ca
2+
)
reactive
oxygen
species
(ROS)
mitochondrion
its
cytosol,
thereby
enhancing
our
understanding
signaling
pathways
cellular
autophagy.
Two
independent
nanosensors
integrated
channels
spatially
isolate
target
from
simultaneously
measure
release
Ca
ROS
with
resolution.
Dynamic
tracking
reveals
direct
lysosomal
rather
than
which
triggers
ROS-induced
mitochondria.
Furthermore,
highly
temporal
concurrent
observations
revealed
second
burst
in
both
not
consistent
change
ROS.
These
dynamic
data
elucidate
potential
role
beneficial
feedback
loop
between
pathway
subsequent
generation
mitochondrial
ML-SA-induced
More
importantly,
innovative
platform
facilitates
detailed
molecular
interactions
mitochondria
adjacent
cytosolic
environment,
hardly
realized
using
current
superresolution
optical
microscopy.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 28, 2025
Abstract
Bone
regeneration
is
synergistically
regulated
by
growth
factors,
which
are
expressed
in
a
coordinated
cascade
of
events.
An
ideal
guided
bone
membrane
(GBRM)
shall
present
barrier
and
antibacterial
functions,
promote
osteogenesis
through
time‐controlled
release
factors.
In
this
study,
therapy‐based
Janus
fibrous
fabricated
coaxial
electrospinning
layer‐by‐layer
self‐assembly
technology
(LBL).
Specifically,
the
oriented
PCL/PLGA
fibers
loaded
with
zinc
oxide
nanoparticles
(ZnO
NPs)
designed
as
outer
layer,
randomly
arranged
core‐shell
Gelatin/PLLA
nanofibers
employed
inner
layer
to
rapidly
aFGF
sustainedly
BMP‐2.
Results
demonstrated
that
achieved
multiple
functions
satisfy
essential
requirement
regeneration,
exhibited
remarkable
ability,
function,
osteoinductive
ability.
Interestingly,
significant
enhancement
oxidative
phosphorylation
(OXPHOS)
major
energy
supply
pathway
decisive
factor
drive
osteogenic
differentiation
BMSCs
induced
membranes.
This
study
provides
novel
strategy
fabricate
multifunctional
membranes/scaffolds,
displaying
great
potential
applications
tissue
engineering.
Besides,
understanding
synergistic
mechanism
factors
on
cellular
metabolism
process
can
provide
deeper
insights
into
factors‐mediated
optimizing
healing
outcomes.
The Journal of Gene Medicine,
Год журнала:
2025,
Номер
27(2)
Опубликована: Фев. 1, 2025
ABSTRACT
Background
Gliomas
currently
have
a
poor
prognosis
and
limited
therapy
options.
Betulinic
acid
(BA)
has
demonstrated
antitumor
activity
in
various
cancers.
This
study
is
aimed
at
clarifying
the
underlying
mechanisms
by
which
BA
inhibits
gliomas.
Methods
We
assessed
how
affected
migration,
apoptosis,
invasion,
proliferation,
viability
of
U251
glioma
cells.
The
genes
that
were
differentially
expressed
after
treatment
identified
via
RNA
sequencing.
Utilizing
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes,
research
was
done
to
determine
pathways.
Molecular
docking
applied
explore
interaction
with
key
pathway
molecules.
Experimental
assays
conducted
confirm
impact
on
these
pathways
targets.
Results
In
cells,
reduced
viability;
inhibited
colony
formation,
invasion;
triggered
apoptosis.
Through
sequencing,
923
up‐
1469
downregulated
found,
notable
enrichment
TNF,
PI3K‐Akt,
ferroptosis
can
stably
bind
TNF
PI3K‐Akt
molecules,
especially
AKT1
(binding
energy
=
−10.2
kcal/mol).
administration
decreased
levels
phosphorylated
PI3K
AKT.
Moreover,
BA‐induced
HO‐1
NRF2
increased.
Ferrostatin‐1
zinc
protoporphyrin
pretreatment
intracellular
iron
lipid
peroxidation
decrease
cell
caused
BA.
Conclusions
controls
PI3K/Akt
NRF2/HO‐1
pathways,
results
ferroptosis.
Understanding
BA's
multipathway
mechanism
may
inform
its
therapeutic
potential
treatment.
Journal of Medicinal Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 6, 2025
Elevated
levels
of
reactive
oxygen
species
(ROS)
in
cancer
cells,
arising
from
their
altered
metabolism,
represent
a
distinctive
therapeutic
target.
Here,
we
introduce
the
first
example
bisboronic
esters
as
potent
agents
for
rapidly
disrupting
redox
homeostasis,
selectively
inducing
oxidative
stress
to
eliminate
cells.
Structure-activity
relationship
studies
revealed
that
both
electronic
and
steric
factors
significantly
influence
cytotoxicity,
with
most
effective
compounds
achieving
IC50
values
low
0.63
μM.
Confocal
imaging
confirmed
rapid
intracellular
depletion
ROS,
leading
severe
metabolic
imbalance
efficient
cell
death.
This
study
highlights
potential
boronate-based
therapeutics
redox-targeted
therapy,
which
may
provide
promising
foundation
development
innovative
anticancer
strategies.
