New Journal of Chemistry, Год журнала: 2024, Номер 48(36), С. 15782 - 15789
Опубликована: Янв. 1, 2024
We developed a label-free colorimetric photothermal bimodal POCT sensing platform based on cascade catalytic signal amplification for the detection of p53.
Язык: Английский
Chemical Reviews, Год журнала: 2024, Номер 124(5), С. 2553 - 2582
Опубликована: Март 4, 2024
The intricate and complex features of enzymatic reaction networks (ERNs) play a key role in the emergence sustenance life. Constructing such vitro enables stepwise build up complexity introduces opportunity to control activity using physicochemical stimuli. Rational design modulation network motifs enable engineering artificial systems with emergent functionalities. Such functional are useful for variety reasons as creating new-to-nature dynamic materials, producing value-added chemicals, constructing metabolic modules synthetic cells, even enabling molecular computation. In this review, we offer insights into chemical characteristics ERNs while also delving their potential applications associated challenges.
Язык: Английский
Процитировано
26
Science Advances, Год журнала: 2025, Номер 11(2)
Опубликована: Янв. 8, 2025
Chemodynamic therapy (CDT) is a promising and potent therapeutic strategy for the treatment of cancer. We developed DNA origami–based enzymatic cascade nanoreactor (DOECN) containing spatially well-organized Au nanoparticles ferric oxide (Fe 2 O 3 ) nanoclusters targeted delivery inhibition tumor cell growth. The DOECN can synergistically promote generation hydrogen peroxide (H ), consumption glutathione, creation an acidic environment, thereby amplifying Fenton-type reaction producing abundant reactive oxygen species, such as hydroxyl radicals (•OH), augmenting CDT outcome. decorated with targeting groups to achieve efficient cellular uptake efficiently induce apoptosis, ferroptosis, immunogenetic death, thus realizing anticancer effects. Intravenous injection effectively promoted maturation dendritic cells, triggered adaptive T responses, suppressed growth in murine cancer model. provides programmable platform integration multiple components, showing great potential combined therapy.
Язык: Английский
Процитировано
2
ACS electrochemistry., Год журнала: 2025, Номер unknown
Опубликована: Фев. 15, 2025
Язык: Английский
Процитировано
2
Environmental Science & Technology, Год журнала: 2024, Номер 58(27), С. 11869 - 11886
Опубликована: Июнь 28, 2024
Developing efficient technologies to eliminate or degrade contaminants is paramount for environmental protection. Biocatalytic decontamination offers distinct advantages in terms of selectivity and efficiency; however, it still remains challenging when applied complex matrices. The main challenge originates from the instability difficult-to-separate attributes fragile enzymes, which also results issues compromised activity, poor reusability, low cost-effectiveness, etc. One viable solution harness biocatalysis environments known as enzyme immobilization, where a flexible tightly fixed solid carrier. In case reticular crystal utilized support, feasible engineer next-generation biohybrid catalysts functional complicated media. This can be interpreted by three aspects: (1) highly crystalline skeleton shield immobilized against external stressors. (2) porous network ensures high accessibility interior catalytic decontamination. And (3) adjustable unambiguous structure framework favors in-depth understanding interfacial interaction between enzyme, turn guide us designing active biocomposites. Review aims introduce this emerging technology involving pollutant degradation greenhouse gas (carbon dioxide) conversion, with emphasis on immobilization protocols diverse catalysis principles including single catalysis, cascades, photoenzyme-coupled catalysis. Additionally, remaining challenges forward-looking directions field are discussed. We believe that may offer useful biocatalytic contribute green sustainable manner will inspire more researchers at intersection environment science, biochemistry, materials science communities co-solve problems.
Язык: Английский
Процитировано
8
Advanced Functional Materials, Год журнала: 2024, Номер 34(18)
Опубликована: Янв. 24, 2024
Abstract Exploring the potential of microfluidic systems, this study presents a groundbreaking approach harnessing energy in flows within purpose‐built microreactor, enabling precise deposition functional biomaterials. Upon optimizing reactor dimensions and integrating it into system, sequentially flow‐induced DNA hydrogels transformation DNA‐protein hybrid materials with SpyTag/SpyCatcher technology is investigated. However, limited functionalization rates restrict its viability for targeted biocatalytic processes. Therefore, direct phenolic acid decarboxylase investigated, which efficiently deposited but shows performance due to shear‐induced denaturation. This challenge overcome by two‐step immobilization process, resulting bioreactors demonstrating initial high space‐time yields up 7000 g L −1 d , whose process stability proves unsatisfactory. exploiting principle immobilize recombinant E. coli cells as living overexpressing biocatalytically relevant enzymes, are produced that show equally continuous whole‐cell catalysis remain constant over periods 10 days. The insights gained offer optimization strategies advanced innovative systems holding promise applications fundamental science, biosensing, scalable production microreactors biocatalysis bioremediation.
Язык: Английский
Процитировано
7
Molecular Systems Design & Engineering, Год журнала: 2024, Номер 9(7), С. 679 - 704
Опубликована: Янв. 1, 2024
Schematic depicting enzyme kinetic enhancement when displayed on a nanoparticle surface. We provide state of the art review this phenomenon describing what is known about how it arises along with examples grouped by nanomaterials.
Язык: Английский
Процитировано
7
Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(45)
Опубликована: Авг. 26, 2024
Abstract Oligo‐adenine (polyA) is primarily known for its critical role in mRNA stability, translational status, and gene regulation. Beyond biological functions, extensive research has unveiled the diverse applications of polyA. In response to environmental stimuli, single polyA strands undergo distinctive structural transitions into secondary configurations, which are reversible upon introduction appropriate counter‐triggers. this review, we systematically summarize recent advances noncanonical structures derived from polyA, including A‐motif duplex, A‐cyanuric acid triplex, A‐coralyne‐A T ⋅ A‐T triplex. The characteristics mechanisms underlying these conformations under specific external stimuli addressed, followed by examples their stimuli‐responsive DNA hydrogels, supramolecular fibre assembly, molecular electronics switches, biosensing bioengineering, payloads encapsulation release, others. A detailed comparison polyA‐derived provided, highlighting features. Furthermore, integrating stimuli‐responsiveness conformational characteristics, advanced material development, such as pH‐cascaded hydrogels fibres exhibiting dynamic adapting cues, introduced. An outlook future developments also discussed. These derived, stimuli‐responsive, enrich arsenal “toolbox”, offering frameworks applications.
Язык: Английский
Процитировано
4
Journal of the American Chemical Society, Год журнала: 2024, Номер 146(43), С. 29621 - 29629
Опубликована: Окт. 17, 2024
Biochemical acyl transfer cascades, such as those initiated by the adenylation of carboxylic acids, are central to various biological processes, including protein synthesis and fatty acid metabolism. Designing cascade reactions in aqueous media remains challenging due need control multiple, sequential a single pot manage stability reactive intermediates. Herein, we developed abiotic cascades using aminoacyl phosphate esters, synthetic counterparts adenylates, drive chemical self-assembly pot. We demonstrated that structural elements amino side chains (aromatic versus aliphatic) significantly influence reactivity half-lives ranging from hours days. This behavior, turn, affects number couplings can achieve network propensity activated intermediate structures. The constructed bifunctional peptide substrates featuring chain nucleophiles. Specifically, aromatic acids facilitate formation transient thioesters, which preorganized into spherical aggregates further couple chimeric assemblies composed esters thioesters. In contrast, aliphatic lack ability form structures, predominantly undergo hydrolysis, bypassing transformations after thioester formation. Additionally, mixtures containing multiple substrates, achieved selective product following distinct pathway favors subsequent through changes self-assembly. By coupling with molecules varying time scales, reaction clocks lifetimes dynamics, facilitating precise temporal regulation.
Язык: Английский
Процитировано
4
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(9), С. 5595 - 5608
Опубликована: Авг. 15, 2024
Analysis of biomarkers in living cells is crucial for deciphering the dynamics as well precise diagnosis diseases. DNA biosensors employ sequences probes to offer insights into cells, and drive progress disease drug development. In this review, we present recent advances detecting cells. The basic structural components signal output method are presented. strategies crossing cell membrane also described, including coincubation, nanocarriers, nanoelectroporation techniques. Based on biomarker categorization, detail applications small molecules, RNAs, proteins, integrated targets Furthermore, future development directions summarized encourage further research growing field.
Язык: Английский
Процитировано
3
Microchimica Acta, Год журнала: 2025, Номер 192(2)
Опубликована: Янв. 13, 2025
Язык: Английский
Процитировано
0