New Journal of Chemistry,
Год журнала:
2024,
Номер
48(36), С. 15782 - 15789
Опубликована: Янв. 1, 2024
We
developed
a
label-free
colorimetric
photothermal
bimodal
POCT
sensing
platform
based
on
cascade
catalytic
signal
amplification
for
the
detection
of
p53.
Chemical Reviews,
Год журнала:
2024,
Номер
124(5), С. 2553 - 2582
Опубликована: Март 4, 2024
The
intricate
and
complex
features
of
enzymatic
reaction
networks
(ERNs)
play
a
key
role
in
the
emergence
sustenance
life.
Constructing
such
vitro
enables
stepwise
build
up
complexity
introduces
opportunity
to
control
activity
using
physicochemical
stimuli.
Rational
design
modulation
network
motifs
enable
engineering
artificial
systems
with
emergent
functionalities.
Such
functional
are
useful
for
variety
reasons
as
creating
new-to-nature
dynamic
materials,
producing
value-added
chemicals,
constructing
metabolic
modules
synthetic
cells,
even
enabling
molecular
computation.
In
this
review,
we
offer
insights
into
chemical
characteristics
ERNs
while
also
delving
their
potential
applications
associated
challenges.
Environmental Science & Technology,
Год журнала:
2024,
Номер
58(27), С. 11869 - 11886
Опубликована: Июнь 28, 2024
Developing
efficient
technologies
to
eliminate
or
degrade
contaminants
is
paramount
for
environmental
protection.
Biocatalytic
decontamination
offers
distinct
advantages
in
terms
of
selectivity
and
efficiency;
however,
it
still
remains
challenging
when
applied
complex
matrices.
The
main
challenge
originates
from
the
instability
difficult-to-separate
attributes
fragile
enzymes,
which
also
results
issues
compromised
activity,
poor
reusability,
low
cost-effectiveness,
etc.
One
viable
solution
harness
biocatalysis
environments
known
as
enzyme
immobilization,
where
a
flexible
tightly
fixed
solid
carrier.
In
case
reticular
crystal
utilized
support,
feasible
engineer
next-generation
biohybrid
catalysts
functional
complicated
media.
This
can
be
interpreted
by
three
aspects:
(1)
highly
crystalline
skeleton
shield
immobilized
against
external
stressors.
(2)
porous
network
ensures
high
accessibility
interior
catalytic
decontamination.
And
(3)
adjustable
unambiguous
structure
framework
favors
in-depth
understanding
interfacial
interaction
between
enzyme,
turn
guide
us
designing
active
biocomposites.
Review
aims
introduce
this
emerging
technology
involving
pollutant
degradation
greenhouse
gas
(carbon
dioxide)
conversion,
with
emphasis
on
immobilization
protocols
diverse
catalysis
principles
including
single
catalysis,
cascades,
photoenzyme-coupled
catalysis.
Additionally,
remaining
challenges
forward-looking
directions
field
are
discussed.
We
believe
that
may
offer
useful
biocatalytic
contribute
green
sustainable
manner
will
inspire
more
researchers
at
intersection
environment
science,
biochemistry,
materials
science
communities
co-solve
problems.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(18)
Опубликована: Янв. 24, 2024
Abstract
Exploring
the
potential
of
microfluidic
systems,
this
study
presents
a
groundbreaking
approach
harnessing
energy
in
flows
within
purpose‐built
microreactor,
enabling
precise
deposition
functional
biomaterials.
Upon
optimizing
reactor
dimensions
and
integrating
it
into
system,
sequentially
flow‐induced
DNA
hydrogels
transformation
DNA‐protein
hybrid
materials
with
SpyTag/SpyCatcher
technology
is
investigated.
However,
limited
functionalization
rates
restrict
its
viability
for
targeted
biocatalytic
processes.
Therefore,
direct
phenolic
acid
decarboxylase
investigated,
which
efficiently
deposited
but
shows
performance
due
to
shear‐induced
denaturation.
This
challenge
overcome
by
two‐step
immobilization
process,
resulting
bioreactors
demonstrating
initial
high
space‐time
yields
up
7000
g
L
−1
d
,
whose
process
stability
proves
unsatisfactory.
exploiting
principle
immobilize
recombinant
E.
coli
cells
as
living
overexpressing
biocatalytically
relevant
enzymes,
are
produced
that
show
equally
continuous
whole‐cell
catalysis
remain
constant
over
periods
10
days.
The
insights
gained
offer
optimization
strategies
advanced
innovative
systems
holding
promise
applications
fundamental
science,
biosensing,
scalable
production
microreactors
biocatalysis
bioremediation.
Molecular Systems Design & Engineering,
Год журнала:
2024,
Номер
9(7), С. 679 - 704
Опубликована: Янв. 1, 2024
Schematic
depicting
enzyme
kinetic
enhancement
when
displayed
on
a
nanoparticle
surface.
We
provide
state
of
the
art
review
this
phenomenon
describing
what
is
known
about
how
it
arises
along
with
examples
grouped
by
nanomaterials.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(45)
Опубликована: Авг. 26, 2024
Abstract
Oligo‐adenine
(polyA)
is
primarily
known
for
its
critical
role
in
mRNA
stability,
translational
status,
and
gene
regulation.
Beyond
biological
functions,
extensive
research
has
unveiled
the
diverse
applications
of
polyA.
In
response
to
environmental
stimuli,
single
polyA
strands
undergo
distinctive
structural
transitions
into
secondary
configurations,
which
are
reversible
upon
introduction
appropriate
counter‐triggers.
this
review,
we
systematically
summarize
recent
advances
noncanonical
structures
derived
from
polyA,
including
A‐motif
duplex,
A‐cyanuric
acid
triplex,
A‐coralyne‐A
T
⋅
A‐T
triplex.
The
characteristics
mechanisms
underlying
these
conformations
under
specific
external
stimuli
addressed,
followed
by
examples
their
stimuli‐responsive
DNA
hydrogels,
supramolecular
fibre
assembly,
molecular
electronics
switches,
biosensing
bioengineering,
payloads
encapsulation
release,
others.
A
detailed
comparison
polyA‐derived
provided,
highlighting
features.
Furthermore,
integrating
stimuli‐responsiveness
conformational
characteristics,
advanced
material
development,
such
as
pH‐cascaded
hydrogels
fibres
exhibiting
dynamic
adapting
cues,
introduced.
An
outlook
future
developments
also
discussed.
These
derived,
stimuli‐responsive,
enrich
arsenal
“toolbox”,
offering
frameworks
applications.
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(43), С. 29621 - 29629
Опубликована: Окт. 17, 2024
Biochemical
acyl
transfer
cascades,
such
as
those
initiated
by
the
adenylation
of
carboxylic
acids,
are
central
to
various
biological
processes,
including
protein
synthesis
and
fatty
acid
metabolism.
Designing
cascade
reactions
in
aqueous
media
remains
challenging
due
need
control
multiple,
sequential
a
single
pot
manage
stability
reactive
intermediates.
Herein,
we
developed
abiotic
cascades
using
aminoacyl
phosphate
esters,
synthetic
counterparts
adenylates,
drive
chemical
self-assembly
pot.
We
demonstrated
that
structural
elements
amino
side
chains
(aromatic
versus
aliphatic)
significantly
influence
reactivity
half-lives
ranging
from
hours
days.
This
behavior,
turn,
affects
number
couplings
can
achieve
network
propensity
activated
intermediate
structures.
The
constructed
bifunctional
peptide
substrates
featuring
chain
nucleophiles.
Specifically,
aromatic
acids
facilitate
formation
transient
thioesters,
which
preorganized
into
spherical
aggregates
further
couple
chimeric
assemblies
composed
esters
thioesters.
In
contrast,
aliphatic
lack
ability
form
structures,
predominantly
undergo
hydrolysis,
bypassing
transformations
after
thioester
formation.
Additionally,
mixtures
containing
multiple
substrates,
achieved
selective
product
following
distinct
pathway
favors
subsequent
through
changes
self-assembly.
By
coupling
with
molecules
varying
time
scales,
reaction
clocks
lifetimes
dynamics,
facilitating
precise
temporal
regulation.
ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(9), С. 5595 - 5608
Опубликована: Авг. 15, 2024
Analysis
of
biomarkers
in
living
cells
is
crucial
for
deciphering
the
dynamics
as
well
precise
diagnosis
diseases.
DNA
biosensors
employ
sequences
probes
to
offer
insights
into
cells,
and
drive
progress
disease
drug
development.
In
this
review,
we
present
recent
advances
detecting
cells.
The
basic
structural
components
signal
output
method
are
presented.
strategies
crossing
cell
membrane
also
described,
including
coincubation,
nanocarriers,
nanoelectroporation
techniques.
Based
on
biomarker
categorization,
detail
applications
small
molecules,
RNAs,
proteins,
integrated
targets
Furthermore,
future
development
directions
summarized
encourage
further
research
growing
field.