Structural and Biophysical Dynamics of Fungal Plasma Membrane Proteins and Implications for Echinocandin Action inCandida glabrata DOI Open Access
Jennifer Jiang, Mikhail V. Keniya,

Anusha Puri

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 30, 2024

Abstract Fungal plasma membrane proteins represent key therapeutic targets for antifungal agents, yet their structure and spatial distribution in the native context remain poorly characterized. Herein, we employ an integrative multimodal approach to elucidate structural functional organization of protein complexes Candida glabrata , focusing on prominent essential proteins, polysaccharide synthase β-(1,3)-glucan (GS) proton pump Pma1. Cryo-electron tomography (cryo-ET) live cell imaging reveal that GS Pma1 are heterogeneously distributed into distinct microdomains. Treatment with caspofungin, echinocandin GS, alters disrupts Based these findings, propose a model action considers how drug interactions environment lead inhibition GS. Our work underscores importance interrogating dynamic characteristics fungal situ understand function facilitate precisely targeted development novel therapies.

Язык: Английский

An Overview of the Recent Advances in Antimicrobial Resistance DOI Creative Commons
Manuela Oliveira, Wilson Antunes,

S. R. F. MOTA

и другие.

Microorganisms, Год журнала: 2024, Номер 12(9), С. 1920 - 1920

Опубликована: Сен. 21, 2024

Antimicrobial resistance (AMR), frequently considered a major global public health threat, requires comprehensive understanding of its emergence, mechanisms, advances, and implications. AMR’s epidemiological landscape is characterized by widespread prevalence constantly evolving patterns, with multidrug-resistant organisms (MDROs) creating new challenges every day. The most common mechanisms underlying AMR (i.e., genetic mutations, horizontal gene transfer, selective pressure) contribute to the emergence dissemination resistant strains. Therefore, mitigation strategies (e.g., antibiotic stewardship programs—ASPs—and infection prevention control strategies—IPCs) emphasize importance responsible antimicrobial use surveillance. A One Health approach interconnectedness human, animal, environmental health) highlights necessity for interdisciplinary collaboration holistic in combating AMR. Advancements novel therapeutics alternative agents vaccines) offer promising avenues addressing challenges. Policy interventions at international national levels also promote ASPs aiming regulate use. Despite all observed progress, remains pressing concern, demanding sustained efforts address emerging threats sustainability. Future research must prioritize innovative approaches complex socioecological dynamics This manuscript resource researchers, policymakers, healthcare professionals seeking navigate develop effective mitigation.

Язык: Английский

Процитировано

33

What is the role of microbial biotechnology and genetic engineering in medicine? DOI Creative Commons
Fernando Santos‐Beneit

MicrobiologyOpen, Год журнала: 2024, Номер 13(2)

Опубликована: Март 31, 2024

Abstract Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and enzymes. Genetic engineering techniques, functional genomics, synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene‐based technologies medical applications.

Язык: Английский

Процитировано

14

Frontiers in superbug management: innovating approaches to combat antimicrobial resistance DOI
Priyanka Chambial, Neelam Thakur, Prudhvi Lal Bhukya

и другие.

Archives of Microbiology, Год журнала: 2025, Номер 207(3)

Опубликована: Фев. 14, 2025

Язык: Английский

Процитировано

0

Illuminating antifungal mode of action and resistance with fluorescent probes DOI Creative Commons
Moriah Jospe‐Kaufman, Micha Fridman

Current Opinion in Chemical Biology, Год журнала: 2025, Номер 85, С. 102570 - 102570

Опубликована: Фев. 17, 2025

Язык: Английский

Процитировано

0

INVASIVE FUNGAL INFECTION CAUSED BY MAGNUSIOMYCES CAPITATUS IN A PEDIATRIC PATIENT WITH BURKITT LYMPHOMA: CASE REPORT AND REVIEW OF LITERATURE DOI
José Antonio Soler-Simón,

Diannet Quintero-García,

Álvaro Pou-Blázquez

и другие.

Journal de Mycologie Médicale, Год журнала: 2025, Номер 35(2), С. 101547 - 101547

Опубликована: Март 29, 2025

Язык: Английский

Процитировано

0

Candida albicans: the current status regarding vaginal infections DOI Creative Commons
Margarida Faustino, Carlos Ferreira, Ana Margarida Pereira

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)

Опубликована: Апрель 10, 2025

Abstract Vaginal infections caused by Candida albicans are a significant global health concern due to their recurrence and negative impact on quality of life. This review examines the pathogenesis C. infections, emphasizing critical virulence factors such as biofilm formation, adherence, phenotypic switching. Risk include immune system suppression, antibiotic use, hormonal changes, all which can lead fungal overgrowth infection. Current prevention and/or treatment strategies primarily rely antifungal therapies, personal hygiene practices, probiotics. However, challenges like resistance, recurrence, limited efficacy highlight need for innovative approaches. Therefore, emerging methods novel agents, vaccines, nanotechnology-based delivery systems offer promising advancements improve infection control. Additionally, plays key role in preventing with both innate adaptive immunity acting restrict colonization growth. Commercially available products, creams, vaginal probiotics, solutions, practical options but often lack long-term efficacy. Persistent challenges, including patient noncompliance, restricted access hinder comprehensive efforts. Thus, future research should focus promoting interdisciplinary approaches, integrating personalized medicine, enhancing healthcare accessibility. intends present current state art within abovementioned issues enhance understanding multifactorial nature advanced strategies, essential reduce burden candidiasis worldwide life outcomes. Key points • involves biofilms, Vaccines, nanotechnology, new drugs improved treatment. Addressing resistance compliance is success. Graphical

Язык: Английский

Процитировано

0

Fungal cell wall biogenesis: structural complexity, regulation and inhibition DOI Creative Commons
Neil A. R. Gow

Fungal Genetics and Biology, Год журнала: 2025, Номер 179, С. 103991 - 103991

Опубликована: Май 5, 2025

Язык: Английский

Процитировано

0

FungAMR: A comprehensive portrait of antimicrobial resistance mutations in fungi DOI Creative Commons
Camille Bédard,

Alicia Pageau,

Anna Fijarczyk

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 7, 2024

Abstract Antimicrobial resistance (AMR) is a global threat. To optimize the use of our antifungal arsenal, we need rapid detection and monitoring tools that rely on high-quality AMR mutation data. Here, performed thorough manual curation published mutations in fungal pathogens to produce FungAMR reference dataset. A total 462 papers were curated, leading 54,666 entries all classified with degree evidence supports their role resistance. covers 92 species, 202 genes 184 drugs. We combined variant effect predictors showed these could be used help predict potential impact AMR. Additionally, comparative analysis among species revealed high level convergence molecular basis resistance, revealing some potentially universal mutations. The also significant number lead cross-resistance within antifungals class, as well between classes for certain mutated genes. acquisition clinic field an urging concern. Finally, provide computational tool, ChroQueTas, leverages screen genomes These resources are anticipated have great utility researchers fight against

Язык: Английский

Процитировано

2

Structural and Biophysical Dynamics of Fungal Plasma Membrane Proteins and Implications for Echinocandin Action inCandida glabrata DOI Open Access
Jennifer Jiang, Mikhail V. Keniya,

Anusha Puri

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 30, 2024

Abstract Fungal plasma membrane proteins represent key therapeutic targets for antifungal agents, yet their structure and spatial distribution in the native context remain poorly characterized. Herein, we employ an integrative multimodal approach to elucidate structural functional organization of protein complexes Candida glabrata , focusing on prominent essential proteins, polysaccharide synthase β-(1,3)-glucan (GS) proton pump Pma1. Cryo-electron tomography (cryo-ET) live cell imaging reveal that GS Pma1 are heterogeneously distributed into distinct microdomains. Treatment with caspofungin, echinocandin GS, alters disrupts Based these findings, propose a model action considers how drug interactions environment lead inhibition GS. Our work underscores importance interrogating dynamic characteristics fungal situ understand function facilitate precisely targeted development novel therapies.

Язык: Английский

Процитировано

1