Integrating Machine Learning with MALDI-TOF Mass Spectrometry for Rapid and Accurate Antimicrobial Resistance Detection in Clinical Pathogens

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1140 - 1140

Опубликована: Янв. 28, 2025

Antimicrobial resistance (AMR) is one of the most pressing public health challenges 21st century. This study aims to evaluate efficacy mass spectral data generated by VITEK® MS instruments for predicting antibiotic in Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae using machine learning algorithms. Additionally, potential pre-trained models was assessed through transfer analysis. A dataset comprising 2229 spectra collected, classification algorithms, including Support Vector Machines, Random Forest, Logistic Regression, CatBoost, were applied predict resistance. CatBoost demonstrated a clear advantage over other models, effectively handling complex non-linear relationships within achieving an AUROC 0.91 F1 score 0.78 E. coli. In contrast, yielded suboptimal results. These findings highlight gradient-boosting techniques enhance prediction, particularly with from less conventional platforms like MS. Furthermore, identification specific biomarkers SHAP values indicates promising clinical applications early diagnosis. Future efforts focused on standardizing refining algorithms could expand utility these approaches across diverse environments, supporting global fight against AMR.

Язык: Английский

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Advancements in Antibacterial Therapy: Feature Papers

Microorganisms, Год журнала: 2025, Номер 13(3), С. 557 - 557

Опубликована: Март 1, 2025

Antimicrobial resistance (AMR) is a growing global health crisis that threatens the efficacy of antibiotics and modern medical interventions. The emergence multidrug-resistant (MDR) pathogens, exacerbated by misuse in healthcare agriculture, underscores urgent need for innovative solutions. (1) Background: AMR arises from complex interactions between human, animal, environmental health, further aggravated overuse inadequate regulation antibiotics. Conventional treatments are increasingly ineffective, necessitating alternative strategies. Emerging approaches, including bacteriophage therapy, antimicrobial peptides (AMPs), nanotechnology, microbial extracellular vesicles (EVs), CRISPR-based antimicrobials, provide novel mechanisms complement traditional combating resistant pathogens. (2) Methods: This review critically analyzes advanced antibacterial strategies conjunction with systemic reforms such as stewardship programs, One Health framework, surveillance tools. These methods can enhance detection, guide interventions, promote sustainable practices. Additionally, economic, logistical, regulatory challenges impeding their implementation evaluated. (3) Results: technologies, CRISPR exhibit promising potential targeting mechanisms. However, disparities resource distribution barriers hinder widespread adoption. Public–private partnerships agriculture practices critical to overcoming these obstacles. (4) Conclusions: A holistic integrated approach essential mitigating impact AMR. By aligning therapeutic policies, fostering interdisciplinary collaboration, ensuring equitable distribution, we develop response this 21st-century challenge.

Язык: Английский

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The Burden of Antimicrobial Resistance in Zambia, a Sub-Saharan African Country: A One Health Review of the Current Situation, Risk Factors, and Solutions

Pharmacology &amp Pharmacy, Год журнала: 2024, Номер 15(12), С. 403 - 465

Опубликована: Янв. 1, 2024

Язык: Английский

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Antibacterial, antibiofilm and anti-quorum sensing activities of 1,2,3,5-tetrazine derivatives linked to a benzothiazole moiety

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Abstract A series of known tetrazine derivatives, containing benzothiazole scaffold, were prepared during the coupling reactions selected diazotized 2-aminobenzo[d]thiazole derivatives with p-acetaminophen. The as-prepared compounds characterized based on NMR and MS spectrometry. antibacterial anti-biofilm activities synthesized evaluated by microdilution method, whereas anti-quorum sensing effect was carried out using assay for inhibition violacein formation. As a result, 4a, 4b 4c revealed minimum inhibitory concentrations bactericidal ranging from 8 to 128 µg/mL 32 256 µg/mL, respectively. Compounds 4a (52-86.5%), (57.7-79.4%) (59.9-80.3%) prevented biofilm formation in all four bacteria tested percentages more than 50%. that inhibited 50% production found be 62.71, 28.56 107.45 respectively, thus attesting these possess activity. Noteworthy, our previous investigation attested are non-cytotoxic human mammalian cells Vero. This novel contribution demonstrates antibacterial, antibiofilm tetrazine-based benzothiazoles, which might prospected as scaffolds discovery efficient antibiotics decreased risk microbial drug resistance.

Язык: Английский

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The Leaves and Seeds of Vigna subterranean (Bambara Nut) Inhibit the Growth of Selected Bacterial and Fungal Organisms

Asian Plant Research Journal, Год журнала: 2025, Номер 13(1), С. 37 - 45

Опубликована: Янв. 16, 2025

With increasing resistance of bacterial organisms to conventional antibiotics, it is crucial that alternative therapies are produced combat this menace. One plant has gathered the attention researchers in recent time as a potential medicinal Bambara nut (Vigna subterranean). Hence, study was aimed at investigating antimicrobial properties Vigna subterranea, commonly known nut, synthetic antibiotics amid rising levels. The employed an vitro experimental design, utilizing agar well diffusion method for qualitative assessment and broth microdilution determine minimum inhibitory concentration (MIC) extracts from seeds leaves plant. findings reveal both leaf seed possess significant activities against various pathogens, including Gram-positive Gram-negative bacteria fungi. comparison results showed extract higher antibacterial effect than extract. However, antifungal activity, whereas activity seen based on zones inhibition, MIC result better. Chemical analysis identified phytochemicals such flavonoids, tannins, alkaloids, which contribute observed activity. This shows subterranea potential, with potency extracts. means components could be developed natural alternatives AMR. In conclusion, these underscore importance further research into plant-based antimicrobials, may provide sustainable solutions growing challenge drug clinical settings.

Язык: Английский

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Antibiotic Resistance Gene Pollution in Poultry Farming Environments and Approaches for Mitigation: a system review

Poultry Science, Год журнала: 2025, Номер unknown, С. 104858 - 104858

Опубликована: Янв. 1, 2025

Язык: Английский

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Green synthesis of silver nanoparticles using Viola odorata and Onosma hispidum: Evaluation of their antimicrobial, antioxidant, and hemolytic properties against multidrug resistant microbes

Electronic Journal of Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Frontiers in superbug management: innovating approaches to combat antimicrobial resistance

Archives of Microbiology, Год журнала: 2025, Номер 207(3)

Опубликована: Фев. 14, 2025

Язык: Английский

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Point Prevalence Survey of Antibiotic Use in Level 1 hospitals in Zambia: Future Prospects for Antimicrobial Stewardship Programs

Infection and Drug Resistance, Год журнала: 2025, Номер Volume 18, С. 887 - 902

Опубликована: Фев. 1, 2025

The inappropriate prescribing and use of antibiotics have contributed to the emergence spread antimicrobial resistance (AMR). In Zambia, there is a paucity information on patterns among hospitalized patients in level 1 hospitals. This study investigated antibiotic five hospitals Lusaka, Zambia. cross-sectional utilized World Health Organization (WHO) Point Prevalence Survey (PPS) methodology in-patients admitted before 08:00 a.m. survey day August 2024. Data were analysed using IBM SPSS version 23.0. prevalence inpatients was 59.0%, with ceftriaxone being most prescribed. Antibiotics prescribed mainly for paediatrics male inpatients. found that 53.0% from Access group while 38.2% Watch Access, Watch, Reserve (AWaRe) classification. Adherence national treatment guidelines 36.0%, empirically without evidence culture sensitivity tests. high low adherence findings this demonstrate need establish strengthen stewardship programs laboratory capacity aid clinicians diagnosing, treating, managing across

Язык: Английский

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In Silico Identification of Potential Clovibactin-like Antibiotics Binding to Unique Cell Wall Precursors in Diverse Gram-Positive Bacterial Strains

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1724 - 1724

Опубликована: Фев. 18, 2025

The rise in multidrug-resistant bacteria highlights the critical need for novel antibiotics. This study explores clovibactin-like compounds as potential therapeutic agents targeting lipid II, a crucial component bacterial cell wall synthesis, using silico techniques. A total of 2624 clovibactin analogs were sourced from PubChem database and screened ProTox 3.0 software based on their ADME-Tox properties, prioritizing candidates with favorable pharmacokinetic profiles minimal toxicity. Molecular docking protocols then employed to assess binding interactions selected II. Our analysis identified Compound 22 particularly promising candidate, exhibiting strong affinity, stable complex formation, high selectivity target. Binding energy analysis, conducted via molecular dynamics simulations, revealed highly negative value -25.50 kcal/mol 22, surpassing that underscoring its efficacy. In addition, was prioritized due exceptional affinity II suggesting lower likelihood adverse effects. These characteristics position candidate further pharmacological development. While our computational results are encouraging, experimental validation is essential confirm efficacy safety these compounds. not only advances understanding but also contributes ongoing efforts combat antimicrobial resistance through innovative antibiotic

Язык: Английский

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