Activatable Molecular Probes With Clinical Promise for NIR‐II Fluorescent Imaging DOI Open Access
Zikang Chen, Yongjie Zhou, Li Li

и другие.

Small, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Abstract The second near‐infrared window (NIR‐II) fluorescence imaging has been widely adopted in basic scientific research and preclinical applications due to its exceptional spatiotemporal resolution deep tissue penetration. Among the various fluorescent agents, organic small‐molecule fluorophores are considered most promising candidates for clinical translation, owing their well‐defined chemical structures, tunable optical properties, excellent biocompatibility. However, many currently available NIR‐II exhibit an “always‐on” signal, which leads background noise compromises diagnostic accuracy during disease detection. Developing activatable probes (AOSFPs) accurately reporting pathological changes is key advancing toward application. This review summarizes rational design strategies AOSFPs based on four core structures (cyanine, hemicyanine, xanthene, BODIPY). These hold substantial potential translation. Furthermore, recent advances bioimaging comprehensively reviewed, offering clear guidance direction further development. Finally, prospective efforts advance outlined.

Язык: Английский

Near-Infrared-II Fluorescent Probes for Analytical Applications: From In Vitro Detection to In Vivo Imaging Monitoring DOI

Sha Liu,

Wenjun Dong, H.-J. Gao

и другие.

Accounts of Chemical Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

ConspectusBiomarkers play a vital role in the regulation of life processes, especially predicting occurrence and development diseases. For early diagnosis precise treatment diseases, it has become necessary significant to detect biomarkers with sensitivity, accuracy, simplicity, convenience, even visualization. Fluorescent-probe-based techniques have been recognized as one most powerful tools for sensitive detection real time imaging biological samples. However, traditional optical probes, mainly including visible probes (400–700 nm) near-infrared I (NIR-I, 700–900 suffer from low poor resolution, strong absorption scattering, high background fluorescence, which hinder effective monitoring biomarkers.Fortunately, past decade witnessed remarkable evolution application fields II (NIR-II, 900–1700 driven by its exceptional characteristics advancement technologies. Leveraging superior penetration capabilities, negligible autofluorescence, extended fluorescence emission wavelengths, NIR-II fluorescent significantly enhance signal-to-noise ratio (SNR) vitro (IVD) temporal resolution vivo imaging. Our team committed design strategy, controlled synthesis, luminous mechanisms, biomedical applications probes. In this Account, we present representative works recent years our group field analytical applications, ranging different various also will further provide general overview First, are fully summarized, tumor marker detection, virus bacteria analysis, cell testing, small-molecule sensing. Second, adequately discussed, ROS gas monitoring, pH sensing, receptor some serious Finally, outline advantages discuss detail challenges well their future development. There is reasonable prospect that technology based on exhibit great potential research clinical disease diagnosis. We hope Account can expand reach into an broader spectrum fields, enhancing impact scientific discovery medical practice.

Язык: Английский

Процитировано

1
Two-plex in vivo molecular imaging in the second near-infrared window for immunotherapeutic response DOI Creative Commons

Yupeng Sun,

Rui Li, Yike Cai

и другие.

Theranostics, Год журнала: 2025, Номер 15(10), С. 4481 - 4494

Опубликована: Март 19, 2025

Tumor-infiltrating CD8+ T cells and programmed death-1 (PD1) levels are critical indicators for tumor immunophenotyping therapeutic decision-making. Noninvasive optical imaging in the second near infrared window (NIR-II) is particularly well-suited investigating biological processes within tumors live mammals, thanks to its deep-tissue penetration superior spatiotemporal resolution. However, vivo NIR-II has primarily been restricted a single probe at time. Methods: Herein, we developed two-plex molecular method utilizing non-overlapping fluorescence emission of indocyanine green (ICG) NIR-IIa (1000-1200 nm) PbS/CdS core-shell quantum dots (QDs) NIR-IIb (1500-1700 nm). By integrating PD1 aptamer-labeled ICG (ICG-Apt-PD1, targeting PD1) CD8 QDs (QDs@Apt-CD8, cells), our enabled simultaneous noninvasive monitoring number tumors. Results: QDs@Apt-CD8 demonstrated excellent ability infiltrating cells, owing strong luminescence high selectivity specificity. This allowed dynamic We observed heterogeneous bio-distributions across different types revealed immunophenotypes. Moreover, findings indicated that low predicted better anti-tumor effect. Conclusions: Such would complement ex biopsy-based diagnostic techniques, it could contribute developing an immune-scoring algorithm offer more precise prediction immunotherapeutic response.

Язык: Английский

Процитировано

0
Ionogels with Excitation Wavelength Dependent Emission and Dynamic Light‐Tracking for Potential Extreme Rescues DOI
Qianqian Zhang,

Song Ma,

Xipeng Yang

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Abstract Photoluminescent materials with excitation wavelength dependent (Ex‐De) emission are promising for the next generation of photo‐response materials. To advance Ex‐De performance beyond conventional systems, new design paradigms needed. Here ionogels developed by introducing ionic liquids as both fluorophores and solvents. The formed copolymerization cross‐linking acrylic acid (AA) 2,2,3,4,4,4‐hexafluorobutyl acrylate (HFBA) in imidazolium liquid. 3D ionogel network tethers cations to polymer backbones, imparting multi‐color characteristics triggered imidazolium‐conjugated aggregates clusterization, while also offering stabilization various conditions. exhibit behaviors fluorescence from blue at 400 nm yellow 575 when irradiated different wavelengths 300 500 nm. remains after 30 days under high relative humidity (90% RH) or immersion organic Vibrant luminescence is observed elevated temperature (100 °C) cryogenic (−20 °C), twisting, bending stretching 500% strain. stability harsh environments provides opportunities applications dynamic camouflage, multiple information encryption, potential extreme rescues. This work presents a concept next‐generation

Язык: Английский

Процитировано

0
Carbazole Engineering in D–A–D Molecules to Improve the NIR-II Performance for Tumor Micrometastasis Imaging DOI
Le Fang,

Rui Ai,

María Eugenia Sandoval‐Salinas

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Июнь 2, 2025

It is important to image tumor micrometastases in fluorescence-guided surgery (FGS), which requires highly emissive materials with a high signal-to-background ratio. As one type of bright NIR-II dye, donor-acceptor-donor (D-A-D) molecules have been widely applied for vivo imaging. However, the brightness D-A-D when encapsulated nanoparticles bioimaging often unsatisfactory due aggregation-caused quenching or decrease absorption. In this study, we introduced carbazole groups molecules, resulted reduction fluorescence aggregated state larger intermolecular distance. Through proper engineering groups, emission spectra could be red-shifted peak beyond 1000 nm, while maintaining strong absorption and quantum yield. The brightest TBTC-4 were successfully micrometastasis imaging mice, metastases as small 0.5 mm diameter, demonstrates their promising clinical application FGS.

Язык: Английский

Процитировано

0
Lysosome-specific near-infrared fluorescent probe with large stokes shift for H2S imaging in U87 cells and brain glioma mice DOI

Songjiao Li,

Ying Liu,

Ting Yang

и другие.

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 426, С. 137109 - 137109

Опубликована: Дек. 10, 2024

Язык: Английский

Процитировано

1
Activatable Molecular Probes With Clinical Promise for NIR‐II Fluorescent Imaging DOI Open Access
Zikang Chen, Yongjie Zhou, Li Li

и другие.

Small, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Abstract The second near‐infrared window (NIR‐II) fluorescence imaging has been widely adopted in basic scientific research and preclinical applications due to its exceptional spatiotemporal resolution deep tissue penetration. Among the various fluorescent agents, organic small‐molecule fluorophores are considered most promising candidates for clinical translation, owing their well‐defined chemical structures, tunable optical properties, excellent biocompatibility. However, many currently available NIR‐II exhibit an “always‐on” signal, which leads background noise compromises diagnostic accuracy during disease detection. Developing activatable probes (AOSFPs) accurately reporting pathological changes is key advancing toward application. This review summarizes rational design strategies AOSFPs based on four core structures (cyanine, hemicyanine, xanthene, BODIPY). These hold substantial potential translation. Furthermore, recent advances bioimaging comprehensively reviewed, offering clear guidance direction further development. Finally, prospective efforts advance outlined.

Язык: Английский

Процитировано

1