Activatable Molecular Probes With Clinical Promise for NIR‐II Fluorescent Imaging DOI Open Access
Zikang Chen, Yongjie Zhou, Li Li

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract The second near‐infrared window (NIR‐II) fluorescence imaging has been widely adopted in basic scientific research and preclinical applications due to its exceptional spatiotemporal resolution deep tissue penetration. Among the various fluorescent agents, organic small‐molecule fluorophores are considered most promising candidates for clinical translation, owing their well‐defined chemical structures, tunable optical properties, excellent biocompatibility. However, many currently available NIR‐II exhibit an “always‐on” signal, which leads background noise compromises diagnostic accuracy during disease detection. Developing activatable probes (AOSFPs) accurately reporting pathological changes is key advancing toward application. This review summarizes rational design strategies AOSFPs based on four core structures (cyanine, hemicyanine, xanthene, BODIPY). These hold substantial potential translation. Furthermore, recent advances bioimaging comprehensively reviewed, offering clear guidance direction further development. Finally, prospective efforts advance outlined.

Language: Английский

Near-Infrared-II Fluorescent Probes for Analytical Applications: From In Vitro Detection to In Vivo Imaging Monitoring DOI

Sha Liu,

Wenjun Dong, H.-J. Gao

et al.

Accounts of Chemical Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

ConspectusBiomarkers play a vital role in the regulation of life processes, especially predicting occurrence and development diseases. For early diagnosis precise treatment diseases, it has become necessary significant to detect biomarkers with sensitivity, accuracy, simplicity, convenience, even visualization. Fluorescent-probe-based techniques have been recognized as one most powerful tools for sensitive detection real time imaging biological samples. However, traditional optical probes, mainly including visible probes (400–700 nm) near-infrared I (NIR-I, 700–900 suffer from low poor resolution, strong absorption scattering, high background fluorescence, which hinder effective monitoring biomarkers.Fortunately, past decade witnessed remarkable evolution application fields II (NIR-II, 900–1700 driven by its exceptional characteristics advancement technologies. Leveraging superior penetration capabilities, negligible autofluorescence, extended fluorescence emission wavelengths, NIR-II fluorescent significantly enhance signal-to-noise ratio (SNR) vitro (IVD) temporal resolution vivo imaging. Our team committed design strategy, controlled synthesis, luminous mechanisms, biomedical applications probes. In this Account, we present representative works recent years our group field analytical applications, ranging different various also will further provide general overview First, are fully summarized, tumor marker detection, virus bacteria analysis, cell testing, small-molecule sensing. Second, adequately discussed, ROS gas monitoring, pH sensing, receptor some serious Finally, outline advantages discuss detail challenges well their future development. There is reasonable prospect that technology based on exhibit great potential research clinical disease diagnosis. We hope Account can expand reach into an broader spectrum fields, enhancing impact scientific discovery medical practice.

Language: Английский

Citations

1
Two-plex in vivo molecular imaging in the second near-infrared window for immunotherapeutic response DOI Creative Commons

Yupeng Sun,

Rui Li, Yike Cai

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(10), P. 4481 - 4494

Published: March 19, 2025

Tumor-infiltrating CD8+ T cells and programmed death-1 (PD1) levels are critical indicators for tumor immunophenotyping therapeutic decision-making. Noninvasive optical imaging in the second near infrared window (NIR-II) is particularly well-suited investigating biological processes within tumors live mammals, thanks to its deep-tissue penetration superior spatiotemporal resolution. However, vivo NIR-II has primarily been restricted a single probe at time. Methods: Herein, we developed two-plex molecular method utilizing non-overlapping fluorescence emission of indocyanine green (ICG) NIR-IIa (1000-1200 nm) PbS/CdS core-shell quantum dots (QDs) NIR-IIb (1500-1700 nm). By integrating PD1 aptamer-labeled ICG (ICG-Apt-PD1, targeting PD1) CD8 QDs (QDs@Apt-CD8, cells), our enabled simultaneous noninvasive monitoring number tumors. Results: QDs@Apt-CD8 demonstrated excellent ability infiltrating cells, owing strong luminescence high selectivity specificity. This allowed dynamic We observed heterogeneous bio-distributions across different types revealed immunophenotypes. Moreover, findings indicated that low predicted better anti-tumor effect. Conclusions: Such would complement ex biopsy-based diagnostic techniques, it could contribute developing an immune-scoring algorithm offer more precise prediction immunotherapeutic response.

Language: Английский

Citations

0
Lysosome-specific near-infrared fluorescent probe with large stokes shift for H2S imaging in U87 cells and brain glioma mice DOI

Songjiao Li,

Ying Liu,

Ting Yang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 426, P. 137109 - 137109

Published: Dec. 10, 2024

Language: Английский

Citations

1
Activatable Molecular Probes With Clinical Promise for NIR‐II Fluorescent Imaging DOI Open Access
Zikang Chen, Yongjie Zhou, Li Li

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract The second near‐infrared window (NIR‐II) fluorescence imaging has been widely adopted in basic scientific research and preclinical applications due to its exceptional spatiotemporal resolution deep tissue penetration. Among the various fluorescent agents, organic small‐molecule fluorophores are considered most promising candidates for clinical translation, owing their well‐defined chemical structures, tunable optical properties, excellent biocompatibility. However, many currently available NIR‐II exhibit an “always‐on” signal, which leads background noise compromises diagnostic accuracy during disease detection. Developing activatable probes (AOSFPs) accurately reporting pathological changes is key advancing toward application. This review summarizes rational design strategies AOSFPs based on four core structures (cyanine, hemicyanine, xanthene, BODIPY). These hold substantial potential translation. Furthermore, recent advances bioimaging comprehensively reviewed, offering clear guidance direction further development. Finally, prospective efforts advance outlined.

Language: Английский

Citations

1