Programming the elongation of mammalian cell aggregates with synthetic gene circuits DOI Creative Commons
Josquin Courte, Christian Chung, Naisargee Jain

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Abstract A key goal of synthetic morphogenesis is the identification and implementation methods to control morphogenesis. One line research use genetic circuits guiding self-organization cell ensembles. This approach has led several recent successes, including cellular rearrangements in 3D via cell-cell adhesion by user-designed artificial circuits. However, employed reach such achievements can still be optimized along three lines: happens hand, structures are spherical, effectors limited adhesion. Here we show identification, a computational framework, for volumetric axial elongation proliferation, tissue fluidity, signaling. We then seek implement this design mammalian aggregates vitro. start identifying growth fluidity vitro . combine these new modules construct complete that behaviors interest space time, resulting measurable deformation an axis depends on engineered signaling modules. Finally, contextualize silico implementations within unified morphospace suggest further elaboration initial family towards more robust programmed elongation. These results integrated vitro/in pipeline demonstrate promising method designing, screening, implementing morphogenesis, opening way programming various user-defined shapes.

Язык: Английский

Three‐dimensional stem cell models of mammalian gastrulation DOI Creative Commons
David A. Turner, Alfonso Martínez Arias

BioEssays, Год журнала: 2024, Номер 46(12)

Опубликована: Авг. 28, 2024

Abstract Gastrulation is a key milestone in the development of an organism. It period cell proliferation and coordinated cellular rearrangement, that creates outline body plan. Our current understanding mammalian gastrulation has been improved by embryo culture, but there are still many open questions difficult to address because intrauterine embryos low number specimens. In case humans, additional difficulties associated with technical ethical challenges. Over last few years, pluripotent stem models being developed have potential become useful tools understand gastrulation. Here we review these special emphasis on gastruloids provide survey methods produce them robustly, their uses, relationship embryos, prospects as well limitations.

Язык: Английский

Процитировано

7
Mucociliary cell type compositions - bridging the gap between genes and emergent tissue functions DOI Creative Commons
Peter Walentek

Cells and Development, Год журнала: 2025, Номер unknown, С. 204019 - 204019

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Towards an integrated view and understanding of embryonic signalling during murine gastrulation DOI Creative Commons

Rhanna R Haantjes,

Jeske Strik,

Jan Visser

и другие.

Cells and Development, Год журнала: 2025, Номер unknown, С. 204028 - 204028

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
The logic of monsters: development and morphological diversity in stem-cell-based embryo models DOI Creative Commons
Dominica Cao, Sumit Garai, James DiFrisco

и другие.

Interface Focus, Год журнала: 2024, Номер 14(5)

Опубликована: Окт. 25, 2024

Organoids and stem-cell-based embryo models (SEMs) are imperfect organ or representations that explore a much larger space of possible forms, morphospace, compared to their

Язык: Английский

Процитировано

2
Morphogenetic constrains in the development of gastruloids: implications for mouse gastrulation DOI Creative Commons
Ulla‐Maj Fiuza, Sara Bonavia, Pau Pascual-Mas

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Mammalian embryonic size is tightly controlled with checkpoints and compensatory mechanisms correcting defects. Here, we take advantage of gastruloids, a stem cell embryoid system not subject to most controls, study the role in emergent properties mammalian embryogenesis. We report that gastruloids exhibit robust morphology transcriptional profiles within range. However, affects dynamics, and, outside range morphogenesis, precision anterior-posterior (AP) axial elongation. Gastruloid elongation exhibits active cellular contractility, requires planar polarity (PCP), adhesion cell-cell contact remodelling. Smaller initiate earlier, correlated an earlier Brachyury polarisation. expression increases tissue fluidity. Axis formation regulated by balance multifoci coalescence timing initiation programme. Sizes beyond can modify relative composition. Very small aggregates have increased neural fate bias, accompanied loss paraxial mesoderm mediated differences Nodal signalling activity.

Язык: Английский

Процитировано

2
Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos DOI Creative Commons

Qi‐Long Ying,

Jennifer Nichols

BioEssays, Год журнала: 2024, Номер 46(12)

Опубликована: Окт. 14, 2024

Abstract Pluripotent stem cell lines derived from preimplantation mouse embryos have opened opportunities for the study of early mammalian development and generation genetically uncompromised material differentiation into specific types. Murine embryonic cells are highly versatile can be engineered introduced host embryos, transferred to recipient females, gestated investigate gene function at multiple levels as well developmental mechanisms, including lineage segregation competition. In this review, we summarize biomedical motivation driving incremental modification culture regimes analyses that advanced research its current state. Ongoing investigation divergent mechanisms processes adopted by other species, such agriculturally beneficial mammals birds, will continue enrich knowledge inform strategies future in vitro models.

Язык: Английский

Процитировано

1
Programming the elongation of mammalian cell aggregates with synthetic gene circuits DOI Creative Commons
Josquin Courte, Christian Chung, Naisargee Jain

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Abstract A key goal of synthetic morphogenesis is the identification and implementation methods to control morphogenesis. One line research use genetic circuits guiding self-organization cell ensembles. This approach has led several recent successes, including cellular rearrangements in 3D via cell-cell adhesion by user-designed artificial circuits. However, employed reach such achievements can still be optimized along three lines: happens hand, structures are spherical, effectors limited adhesion. Here we show identification, a computational framework, for volumetric axial elongation proliferation, tissue fluidity, signaling. We then seek implement this design mammalian aggregates vitro. start identifying growth fluidity vitro . combine these new modules construct complete that behaviors interest space time, resulting measurable deformation an axis depends on engineered signaling modules. Finally, contextualize silico implementations within unified morphospace suggest further elaboration initial family towards more robust programmed elongation. These results integrated vitro/in pipeline demonstrate promising method designing, screening, implementing morphogenesis, opening way programming various user-defined shapes.

Язык: Английский

Процитировано

0