M⁶A reader protein YTHDF3 regulates cardiomyocyte death and atrophy by modulating the alternative splicing program

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Abstract Background The functional impact of m 6 A modifications on RNA is governed by reader proteins that read the marks and process accordingly. Recent studies have highlighted importance in heart. However, function a protein Ythdf3 heart remains completely unknown. Objective We aim to uncover hearts. Methods& Results Here, we show indispensable for heart, its knockdown leads cardiac cachexia evident cardiomyocyte atrophy, death, less dense myofibrils visualized electron microscopy. also found downregulation response doxorubicin, overexpression rescues doxorubicin-induced atrophy death. Contrary primarily cytoplasmic localization, localizes nucleus. Furthermore, using co-immunoprecipitation coupled with LC-MS/MS, interacts splicing like DDX5 HNRNPU regulates alternative Mechanistically, CaMKIIδ, an increase CaMKIIδA CaMKIIδC isoforms while decrease CaMKIIδ9 isoform. Conclusion Collectively, adult due alteration program.

Язык: Английский

m6A modification profiles of the CHO cells with differential recombinant protein expression using MeRIP-seq/RNA-seq

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143429 - 143429

Опубликована: Апрель 1, 2025

Язык: Английский

Novel and effective screening system for recombinant protein production in CHO cells

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Сен. 6, 2024

Язык: Английский