M⁶A reader protein YTHDF3 regulates cardiomyocyte death and atrophy by modulating the alternative splicing program

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Background The functional impact of m 6 A modifications on RNA is governed by reader proteins that read the marks and process accordingly. Recent studies have highlighted importance in heart. However, function a protein Ythdf3 heart remains completely unknown. Objective We aim to uncover hearts. Methods& Results Here, we show indispensable for heart, its knockdown leads cardiac cachexia evident cardiomyocyte atrophy, death, less dense myofibrils visualized electron microscopy. also found downregulation response doxorubicin, overexpression rescues doxorubicin-induced atrophy death. Contrary primarily cytoplasmic localization, localizes nucleus. Furthermore, using co-immunoprecipitation coupled with LC-MS/MS, interacts splicing like DDX5 HNRNPU regulates alternative Mechanistically, CaMKIIδ, an increase CaMKIIδA CaMKIIδC isoforms while decrease CaMKIIδ9 isoform. Conclusion Collectively, adult due alteration program.

Language: Английский

m6A modification profiles of the CHO cells with differential recombinant protein expression using MeRIP-seq/RNA-seq

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143429 - 143429

Published: April 1, 2025

Language: Английский

Novel and effective screening system for recombinant protein production in CHO cells

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 6, 2024

Language: Английский