Streamlining Bacterial Gene Regulation via Nucleic Acid Delivery with Gold Nanoclusters DOI
Qingsong Zhang, Xinyi Leng, Lin Peng

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Abstract Delivery of exogenous nucleic acids (NAs) for gene regulation in bacteria, bypassing the barrier cell wall, is essential advancing fundamental microbiology and genetic engineering, treatment bacterial diseases. However, current methods that rely on electrical or chemical interventions are limited by their complexity, specialized expertise, laboratory‐specific instrumentation. This study explores capability gold nanoclusters (AuNCs) as carriers delivering small‐interfering RNA antisense oligonucleotides into bacteria targeted while shielding them from degradation during transport. By enhancing cytoplasmic membrane permeability, AuNCs enable efficient internalization NAs both Gram‐positive Gram‐negative exerting negligible influence activity. It demonstrated rationally designed can be released within enabling ~70% knockdown mecA Methicillin‐resistant Staphylococcus aureus (MRSA). significantly reduces MRSA's antibiotic resistance enhances oxacillin efficacy. Furthermore, successful silencing ligA Escherichia coli pilQ Pseudomonas aeruginosa highlights broad adaptability approach across diverse species. The AuNCs‐based next‐generation NA delivery system has potential to transform regulation—previously restricted laboratory settings—into a versatile scalable solution real‐world application.

Язык: Английский

Antimicrobial properties of carbon “quantum” dots for food safety applications DOI Creative Commons
J.V. Collins, Liju Yang, Xiuli Dong

и другие.

Journal of Nanoparticle Research, Год журнала: 2025, Номер 27(2)

Опубликована: Янв. 31, 2025

Abstract Carbon dots represent a rapidly advancing and expanding research field, with large number of literature reports on their potential technological applications including those relevant to food safety. In this article, the dot samples prepared by deliberate chemical functionalization preexisting small carbon nanoparticles or thermal carbonization various organic precursors under different processing conditions are highlighted critiqued for similarities differences in sample structure-morphology properties, especially antimicrobial properties safety–related uses. Also discussed representative recent examples use inactivate foodborne pathogens, disrupt biofilms prevent formation, extend shelf life products, which involve antibacterial mechanisms. Some perspectives further development dots–based/derived platform related excellent application opportunities safety provided.

Язык: Английский

Процитировано

1

Green synthesis of highly luminous lemon juice-based carbon dots for antimicrobial assessment and fingerprint detection DOI
Arshad Hussain Wazir, Qudrat Ullah Khan, Faizan Ullah

и другие.

International Journal of Materials Research (formerly Zeitschrift fuer Metallkunde), Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Abstract Carbon dots are regarded as a brand new class of nanostructures in the carbonaceous family that have piqued curiosity researchers wide range bio applications. This work focuses on synthesis and characterization carbon dots, well their latent fingerprint detection antibacterial/antifungal capabilities. Highly luminous were prepared by optimizing simple hydrothermal carbonization settings at 180 °C for 12 h using lemon juice raw precursor. The resulting product was examined Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, diffractometery, ultraviolet–visible spectrophotometer. as-prepared found to be extremely bright when excited under ultraviolet light ( λ = 365 nm). presence oxygen functionalities surface revealed spectrocopy. diffraction pattern confirmed amorphous structure with an average size 7 nm determined Scherrer equation. morphology analysis exhibited aggregated form irregular spherical shapes. chemical examination validated elemental makeup juice-based dots. fingerprints yielded positive results. In addition, obtained displayed antifungal antibacterial activity against tested pathogenic fungal bacterial strains.

Язык: Английский

Процитировано

0

Nanodot‐Inspired Precise Bacterial Gene Suppression in a Smart Hydrogel Bandage for Underwater Wound Healing DOI Creative Commons
Qingsong Zhang, Mingxia Lu,

Richang Ou

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Abstract The complex and dynamic nature of aquatic ecosystems, particularly in marine environments, makes managing wound infections a significant challenge for individuals engaged underwater activities organisms. Although antibiotics have played critical role safeguarding humans health, their risk drug resistance environmental impact present substantial obstacles to long‐term sustainability. Using fin rot disease turbot ( Scophthalmus maximus ) caused by infection Vibrio anguillarum V. as model, new strategy is presented that employs carbon dot (CD)‐based antisense oligonucleotide (ASO) delivery system, combined with an adhesive hydrogel, achieve targeted gene silencing healing. CDs cause enhanced cytoplasmic membrane permeability, efficiently deliver ASOs into without requiring additional equipment or chemical facilitators. specific design the ASO sequence enables empA , achieving efficiency high 71.2%. An hydrogel applied boost local concentration ASO/CDs at sites seawater, effectively sealing infected area preventing turbot. This study pioneer bacterial regulation using CD‐based integrated bandage, offering practical solutions diseases.

Язык: Английский

Процитировано

0

Streamlining Bacterial Gene Regulation via Nucleic Acid Delivery with Gold Nanoclusters DOI
Qingsong Zhang, Xinyi Leng, Lin Peng

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Abstract Delivery of exogenous nucleic acids (NAs) for gene regulation in bacteria, bypassing the barrier cell wall, is essential advancing fundamental microbiology and genetic engineering, treatment bacterial diseases. However, current methods that rely on electrical or chemical interventions are limited by their complexity, specialized expertise, laboratory‐specific instrumentation. This study explores capability gold nanoclusters (AuNCs) as carriers delivering small‐interfering RNA antisense oligonucleotides into bacteria targeted while shielding them from degradation during transport. By enhancing cytoplasmic membrane permeability, AuNCs enable efficient internalization NAs both Gram‐positive Gram‐negative exerting negligible influence activity. It demonstrated rationally designed can be released within enabling ~70% knockdown mecA Methicillin‐resistant Staphylococcus aureus (MRSA). significantly reduces MRSA's antibiotic resistance enhances oxacillin efficacy. Furthermore, successful silencing ligA Escherichia coli pilQ Pseudomonas aeruginosa highlights broad adaptability approach across diverse species. The AuNCs‐based next‐generation NA delivery system has potential to transform regulation—previously restricted laboratory settings—into a versatile scalable solution real‐world application.

Язык: Английский

Процитировано

0