Frontiers in Cellular and Infection Microbiology,
Год журнала:
2022,
Номер
12
Опубликована: Окт. 12, 2022
Anti-infection
strategies
against
pleural
empyema
include
the
use
of
antibiotics
and
drainage
treatments,
but
bacterial
eradication
rates
remain
low.
A
major
challenge
is
formation
biofilms
in
cavity.
DNase
has
antibiofilm
efficacy
vitro
,
intrapleural
therapy
with
recommended
to
treat
empyema,
relevant
mechanisms
limited.
Our
aim
was
investigate
whether
I
inhibit
early
biofilm
Pseudomonas
aeruginosa
-
or
Staphylococcus
aureus
-induced
models.
We
used
various
assays,
such
as
crystal
violet
staining,
confocal
laser
scanning
microscopy
(CLSM)
analysis,
peptide
nucleic
acid-fluorescence
situ
hybridization
(PNA-FISH),
electron
(SEM)
analysis.
results
suggested
that
significantly
inhibited
a
dose-dependent
manner,
without
affecting
growth
P.
S.
.
CLSM
analysis
confirmed
decreased
biomass
thickness
both
biofilms.
The
PNA-FISH
SEM
analyses
also
revealed
(24h)
two
Thus,
indicated
rabbit
models
showed
its
therapeutic
potential
npj Biofilms and Microbiomes,
Год журнала:
2023,
Номер
9(1)
Опубликована: Сен. 7, 2023
Bacterial
biofilms,
which
consist
of
three-dimensional
extracellular
polymeric
substance
(EPS),
not
only
function
as
signaling
networks,
provide
nutritional
support,
and
facilitate
surface
adhesion,
but
also
serve
a
protective
shield
for
the
residing
bacterial
inhabitants
against
external
stress,
such
antibiotics,
antimicrobials,
host
immune
responses.
Biofilm-associated
infections
account
65-80%
all
human
microbial
that
lead
to
serious
mortality
morbidity.
Tremendous
effort
has
been
spent
address
problem
by
developing
biofilm-dispersing
agents
discharge
colonized
cells
more
vulnerable
planktonic
state.
Here,
we
discuss
recent
progress
enzymatic
eradicating
strategies
medical
with
focus
on
dispersal
mechanisms.
Particularly,
review
three
enzyme
classes
have
extensively
investigated,
namely
glycoside
hydrolases,
proteases,
deoxyribonucleases.
Ecotoxicology and Environmental Safety,
Год журнала:
2023,
Номер
264, С. 115389 - 115389
Опубликована: Авг. 25, 2023
Bacteria
that
cause
infectious
diseases
adopt
biofilms
as
one
of
their
most
prevalent
lifestyles.
Biofilms
enable
bacteria
to
tolerate
environmental
stress
and
evade
antibacterial
agents.
This
bacterial
defense
mechanism
has
rendered
the
use
antibiotics
ineffective
for
treatment
diseases.
However,
many
highly
drug-resistant
microbes
have
rapidly
emerged
owing
such
treatments.
Different
signaling
mechanisms
regulate
biofilm
formation,
including
cyclic
dinucleotide
(c-di-GMP),
small
non-coding
RNAs,
quorum
sensing
(QS).
A
cell
density-dependent
phenomenon,
QS
is
associated
with
c-di-GMP
(a
global
messenger),
which
regulates
gene
expression
related
adhesion,
extracellular
matrix
production,
transition
from
planktonic
stage,
stability,
pathogenicity,
virulence,
acquisition
nutrients.
The
article
aims
provide
information
on
inhibiting
formation
disintegrating
mature/preformed
biofilms.
enables
antimicrobials
target
free-living/exposed
cells
at
lower
concentrations
than
those
needed
treat
within
biofilm.
Therefore,
a
complementary
action
antibiofilm
antimicrobial
agents
can
be
robust
strategic
approach
dealing
Taken
together,
these
molecules
broad
implications
human
health.
Antimicrobial Resistance and Infection Control,
Год журнала:
2023,
Номер
12(1)
Опубликована: Сен. 7, 2023
Biofilms
are
ubiquitous
in
healthcare
settings.
By
nature,
biofilms
less
susceptible
to
antimicrobials
and
associated
with
healthcare-associated
infections
(HAI).
Resistance
of
biofilm
is
multifactorial
the
presence
a
matrix
composed
extracellular
polymeric
substances
eDNA,
being
major
contributing
factor.
The
usual
multispecies
composition
environmental
can
also
impact
on
antimicrobial
efficacy.
In
settings,
two
main
types
present:
hydrated
biofilms,
for
example,
drains
parts
some
medical
devices
equipment,
dry
(DSB)
surfaces
possibly
devices.
act
as
reservoir
pathogens
including
multi-drug
resistant
organisms
their
elimination
requires
different
approaches.
control
(drain)
should
be
informed
by
reduction
or
microbial
bioburden
together
measuring
regrowth
time.
DSB
measured
combination
decrease
bacterial
transfer
post-intervention.
Failure
increases
risk
HAI,
but
not
solely
responsible
disinfection
failure
shortcoming.
limited
number
standardised
efficacy
tests
hindrance
end
users
manufacturers,
whilst
Europe
there
no
approved
standard
protocols.
Education
stakeholders
about
ad
hoc
tests,
often
academic
thus
paramount,
achieve
better
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 19, 2024
Biofilms,
which
are
complexes
of
microorganisms
that
adhere
to
surfaces
and
secrete
protective
extracellular
matrices,
wield
substantial
influence
across
diverse
domains
such
as
medicine,
industry,
environmental
science.
Despite
ongoing
challenges
posed
by
biofilms
in
clinical
research
this
field
remains
dynamic
indeterminate.
This
article
provides
a
contemporary
assessment
their
treatment,
with
focus
on
recent
advances,
chronicle
the
evolving
landscape
biofilm
research.
International Journal of Pharmaceutics,
Год журнала:
2024,
Номер
655, С. 124046 - 124046
Опубликована: Март 29, 2024
Typical
antibiotic
treatments
are
often
ineffectual
against
biofilm-related
infections
since
bacteria
residing
within
biofilms
have
developed
various
mechanisms
to
resist
antibiotics.
To
overcome
these
limitations,
antimicrobial-loaded
liposomal
nanoparticles
a
promising
anti-biofilm
strategy
as
they
demonstrated
improved
delivery
and
eradication
of
in
biofilms.
Antibiotic-loaded
revealed
remarkably
higher
antibacterial
activities
than
free
drugs
experimental
settings.
Moreover,
can
be
used
efficaciously
for
the
combinational
antibiotics
other
antimicrobial
compounds/peptide
which
facilitate,
instance,
significant
breakdown
biofilm
matrix,
increased
bacterial
elimination
from
depletion
metabolic
activity
pathogens.
Drug-loaded
liposomes
mitigated
recurrent
considered
tool
address
challenges
associated
resistance.
Furthermore,
it
has
been
that
surface
charge
polyethylene
glycol
modification
notable
impact
on
their
activity.
Future
investigations
should
tackle
persistent
hurdles
with
development
safe
effective
clinical
application
investigate
novel
treatments,
including
CRISPR-Cas
gene
editing,
natural
compounds,
phages,
nano-mediated
approaches.
Herein,
we
emphasize
significance
inhibition
biofilms,
challenges,
recent
advances,
future
perspectives.
