Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160186 - 160186
Опубликована: Фев. 1, 2025
Язык: Английский
Advanced Materials, Год журнала: 2024, Номер 36(35)
Опубликована: Июнь 25, 2024
The compact three-dimensional (3D) structure of extracellular polymeric substances (EPS) within biofilms significantly hinders the penetration antimicrobial agents, making biofilm eradication challenging and resulting in persistent biofilm-associated infections. To address this challenge, a solution is proposed: probiotic bio-heterojunction (P-bioHJ) combining Lactobacillus rhamnosus with MXene (Ti
Язык: Английский
Процитировано
25
Pathogens, Год журнала: 2024, Номер 13(5), С. 393 - 393
Опубликована: Май 8, 2024
Medical devices such as venous catheters (VCs) and urinary (UCs) are widely used in the hospital setting. However, implantation of these is often accompanied by complications. About 60 to 70% nosocomial infections (NIs) linked biofilms. The main complication ability microorganisms adhere surfaces form biofilms which protect them help persist host. Indeed, crossing skin barrier, insertion VC inevitably allows flora or accidental environmental contaminants access underlying tissues cause fatal complications like bloodstream (BSIs). In fact, 80,000 central catheters-BSIs (CVC-BSIs)-mainly occur intensive care units (ICUs) with a death rate 12 25%. Similarly, catheter-associated tract (CA-UTIs) most commonlyhospital-acquired (HAIs) worldwide.These represent up 40% NIs.In this review, we present summary biofilm formation steps. We provide an overview two important clinical settings medical devices, namely catheter-asociated (CA-BSIs) (CA-UTIs), highlight also multidrug resistant bacteria implicated infections. Furthermore, draw attention toseveral useful prevention strategies, advanced antimicrobial antifouling approaches developed reduce bacterial colonization on catheter incidence catheter-related
Язык: Английский
Процитировано
22
Life, Год журнала: 2025, Номер 15(1), С. 49 - 49
Опубликована: Янв. 2, 2025
Biofilms, composed of structured communities bacteria embedded in a self-produced extracellular matrix, pose significant challenge due to their heightened resistance antibiotics and immune responses. This review highlights the mechanisms underpinning antibiotic within bacterial biofilms, elucidating adaptive strategies employed by microorganisms withstand conventional antimicrobial agents. encompasses role altered gene expression, formation persister cells, contributing recalcitrance biofilms eradication. A comprehensive understanding these provides for exploring innovative therapeutic interventions. study explores promising avenues future research, emphasizing necessity uncovering specific genetic phenotypic adaptations occurring biofilms. The identification vulnerabilities biofilm architecture elucidation key biofilm-specific targets emerge as crucial focal points development targeted strategies. In addressing limitations traditional antibiotics, this discusses approaches. Nanomaterials with inherent properties, quorum-sensing inhibitors disrupting communication, bacteriophages viral agents are highlighted potential alternatives. exploration combination therapies, involving agents, biofilm-disrupting enzymes, immunomodulators, is emphasized enhance efficacy existing treatments overcome resilience.
Язык: Английский
Процитировано
2
ACS Infectious Diseases, Год журнала: 2024, Номер 10(5), С. 1483 - 1519
Опубликована: Май 1, 2024
The development of effective antibacterial solutions has become paramount in maintaining global health this era increasing bacterial threats and rampant antibiotic resistance. Traditional antibiotics have played a significant role combating infections throughout history. However, the emergence novel resistant strains necessitates constant innovation research. We analyzed data on antibacterials from CAS Content Collection, largest human-curated collection published scientific knowledge, which proven valuable for quantitative analysis knowledge. Our focuses mining Collection recent publications (since 2012). This article aims to explore intricate landscape research while reviewing advancement traditional emerging strategies. By delving into resistance mechanisms, paper highlights need find alternate strategies address growing concern.
Язык: Английский
Процитировано
14
Pharmaceutics, Год журнала: 2024, Номер 16(3), С. 396 - 396
Опубликована: Март 14, 2024
The opportunistic bacteria growing in biofilms play a decisive role the pathogenesis of chronic infectious diseases. Biofilm-dwelling behave differently than planktonic and are likely to increase resistance tolerance antimicrobial therapeutics. Antimicrobial adjuvants have emerged as promising strategy combat (AMR) restore efficacy existing antibiotics. A combination antibiotics potential adjuvants, (e.g., extracellular polymeric substance (EPS)-degrading enzymes quorum sensing inhibitors (QSI) can improve effects potentially reduce bacterial resistance). In addition, encapsulation antimicrobials within nanoparticulate systems their stability delivery into biofilms. Lipid nanocarriers (LNCs) been established having with adjuvants. Among them, liquid crystal nanoparticles (LCNPs), liposomes, solid lipid (SLNs), nanostructured carriers (NLCs) due superior properties compared traditional formulations, including greater biocompatibility, higher drug loading capacity, protection from chemical or enzymatic degradation, controlled release, targeted delivery, ease preparation, scale-up feasibility. This article reviews recent advances developing various LNCs co-deliver some well-studied combined different classes. treatments is against biofilms, synergistic therapeutics that deserve further investigation also highlighted. review identifies for therapies development. It discusses how LNC-enabled co-delivery advance current clinical treatments, leading innovative products, enabling reuse antibiotics, providing opportunities saving millions lives infections.
Язык: Английский
Процитировано
13
Molecules, Год журнала: 2024, Номер 29(3), С. 558 - 558
Опубликована: Янв. 23, 2024
The development of antibiotic-resistant microorganisms is a major global health concern. Recently, there has been an increasing interest in antimicrobial peptides as therapeutic option. This study aimed to evaluate the triple-action (broad-spectrum antibacterial, anti-biofilm, and anti-quorum sensing activities) melittin, membrane-active peptide present bee venom. minimum inhibitory concentration bactericidal melittin were determined using microdilution method agar plate counting. Growth curve analysis revealed that showed concentration-dependent antibacterial activity. Scanning electron microscope treatment altered morphology. Confocal laser scanning increased membrane permeability intracellular ROS generation bacteria, all which contribute bacterial cell death. In addition, crystal violet (CV) assay was used test anti-biofilm CV demonstrated inhibited biofilm formation eradicated mature biofilms. Biofilm mediated by quorum (QS) plays role this regard, so molecular docking dynamics confirmed interacts with LasR receptors through hydrogen bonds, further evaluates anti-QS activity production virulence factors (pyocyanin, elastase, rhamnolipid), exopolysaccharides secretion, motility, may be key inhibiting mechanism. findings highlight promising broad-spectrum agent, potential QS inhibitor, providing new perspective theoretical basis for alternative antibiotics.
Язык: Английский
Процитировано
9
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 265, С. 130994 - 130994
Опубликована: Март 21, 2024
Язык: Английский
Процитировано
8
Food Control, Год журнала: 2025, Номер unknown, С. 111134 - 111134
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Life Sciences, Год журнала: 2024, Номер 353, С. 122920 - 122920
Опубликована: Июль 22, 2024
Язык: Английский
Процитировано
6
Опубликована: Июль 23, 2024
The extracellular matrix of most bacterial biofilms contains polysaccharides, proteins, and nucleic acids. These biopolymers have been shown to mediate fundamental biofilm-related phenotypes including surface attachment, intercellular adhesion, biocide resistance. Enzymes that degrade polymeric biofilm components, glycoside hydrolases, proteases, nucleases, are useful tools for studying the structure function components also being investigated as potential antibiofilm agents clinical use. Dispersin B is a well-studied, broad spectrum hydrolase produced by Aggregatibacter actinomycetemcomitans. degrades poly-N-acetylglucosamine, polysaccharide mediates formation, stress tolerance, resistance in numerous Gram-negative Gram-positive pathogens. has inhibit pellicle formation; detach pre-formed biofilms; disaggregate flocs; sensitize detachment enzymes, detergents, metal chelators; killing antiseptics, antibiotics, bacteriophages, macrophages, predatory bacteria. This review summarizes results nearly 100 vitro vivo studies carried out on dispersin since it’s discovery 20 years ago. include investigations into biological enzyme, mechanism action, its activities against species. Also discussed applications B.
Язык: Английский
Процитировано
5